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Glucosyltransferases, encoded by\n                    <jats:italic>gtfB\/C\/D<\/jats:italic>\n                    genes in\n                    <jats:italic>S. mutans<\/jats:italic>\n                    , are responsible for producing biofilm exopolysaccharides (EPS) and are considered to be critical virulence factors. Previous studies have highlighted the roles of various regulatory factors of\n                    <jats:italic>gtf<\/jats:italic>\n                    genes in\n                    <jats:italic>S. mutans<\/jats:italic>\n                    . Here, we investigated the role of the global transcriptional regulator CcpA encoded by\n                    <jats:italic>ccpA<\/jats:italic>\n                    in regulating the EPS synthesis and biofilm formation of\n                    <jats:italic>S. mutans<\/jats:italic>\n                    . A\n                    <jats:italic>ccpA<\/jats:italic>\n                    in\u2010frame deletion strain was observed to develop shiny, round colonies and longer cell length. In addition, the deletion of\n                    <jats:italic>ccpA<\/jats:italic>\n                    resulted in impaired growth, diminished synthesis of EPS, and reduced biofilm formation. Transcriptome analysis revealed that differentially expressed genes in the\n                    <jats:italic>ccpA<\/jats:italic>\n                    deletion strain were significantly enriched in pathways of carbohydrate transport and metabolism, in which the expressions of\n                    <jats:italic>gtfB<\/jats:italic>\n                    and\n                    <jats:italic>gtfC<\/jats:italic>\n                    were downregulated markedly. Electrophoretic mobility shift assays confirmed that CcpA directly binds to the promoter sequences of\n                    <jats:italic>gtfB<\/jats:italic>\n                    and\n                    <jats:italic>gtfC<\/jats:italic>\n                    , with a higher affinity for\n                    <jats:italic>gtfC<\/jats:italic>\n                    . Moreover, the expression level of\n                    <jats:italic>ccpA<\/jats:italic>\n                    in part explained differences in the ability to synthesize sufficient EPS and form stable biofilm in clinically isolated strains. These findings highlight that CcpA plays a crucial role in the EPS production and biofilm formation of\n                    <jats:italic>S. mutans<\/jats:italic>\n                    through directly binding to the promoter regions of\n                    <jats:italic>gtfB<\/jats:italic>\n                    and\n                    <jats:italic>gtfC<\/jats:italic>\n                    . This study provides novel insights into the pathogenic mechanisms of\n                    <jats:italic>S. mutans<\/jats:italic>\n                    and potential strategies for the prevention and treatment of dental caries.\n                  <\/jats:p>","DOI":"10.1111\/omi.70027","type":"journal-article","created":{"date-parts":[[2026,3,21]],"date-time":"2026-03-21T05:00:06Z","timestamp":1774069206000},"page":"165-178","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<i>Streptococcus mutans<\/i>\n                    CcpA Promotes Biofilm Exopolysaccharide Production and Virulence Gene Expression"],"prefix":"10.1111","volume":"41","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-9497-1021","authenticated-orcid":false,"given":"Yujie","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Endodontics Shanghai Ninth People's Hospital Shanghai Jiao Tong University School of Medicine  Shanghai People's Republic of China"},{"name":"College of Stomatology Shanghai Jiao Tong University  Shanghai People's Republic of China"},{"name":"National Clinical Research Center for Oral Diseases National Center for Stomatology  Shanghai People's Republic of China"},{"name":"Shanghai Key Laboratory of Stomatology  Shanghai People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhanyi","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Endodontics Shanghai Ninth People's Hospital Shanghai Jiao Tong University School of Medicine  Shanghai People's Republic of China"},{"name":"College of Stomatology Shanghai Jiao Tong University  Shanghai People's Republic of China"},{"name":"National Clinical Research Center for Oral Diseases National Center for Stomatology  Shanghai People's Republic of China"},{"name":"Shanghai Key Laboratory of Stomatology  Shanghai People's Republic of 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