{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T09:11:58Z","timestamp":1778663518215,"version":"3.51.4"},"reference-count":54,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2013,11,20]],"date-time":"2013-11-20T00:00:00Z","timestamp":1384905600000},"content-version":"vor","delay-in-days":19,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Orthopaedic Surgery"],"published-print":{"date-parts":[[2013,11]]},"abstract":"Objective<\/jats:title>To investigate the influence of bone marrow stromal stem cell (BMSCs<\/jats:styled-content>) transplantation on healing of fractures combined with central nerve injuries in rats.<\/jats:p><\/jats:sec>Methods<\/jats:title>\u2002Forty\u2010eight healthy adult SD<\/jats:styled-content> male rats were randomly divided into the following three groups (16 rats in each group): group A<\/jats:styled-content>, simple (left) tibial fracture; group B<\/jats:styled-content>, tibial fracture combined with T<\/jats:styled-content>10<\/jats:sub> spinal cord transection (SCT<\/jats:styled-content>); group C<\/jats:styled-content>, tibial fracture combined with T<\/jats:styled-content>10<\/jats:sub> SCT<\/jats:styled-content> and BMSCs<\/jats:styled-content> transplantation. The tibial fractures were stabilized with modular intramedullary nails and all operated hind limbs were further immobilized in plaster casts to prevent unequal load bearing. BMSCs<\/jats:styled-content> were labeled with bromodeoxyuridine and implanted into the fractures of C<\/jats:styled-content> group rats 2 days after creation of the model. The animals in B<\/jats:styled-content> and C<\/jats:styled-content> groups were evaluated by postoperative Tarlov<\/jats:styled-content> scores. The fractured tibiae were evaluated separately radiographically (X<\/jats:styled-content>\u2010ray and CT<\/jats:styled-content>) and immunohistochemically 1, 2, 3 and 4 weeks after injury to assess fracture healing. In addition, the wet weights of the left tibias were measured.<\/jats:p><\/jats:sec>Results<\/jats:title>All Tarlov<\/jats:styled-content> score of the B<\/jats:styled-content> and C<\/jats:styled-content> group animals reached the requirements of the experiment. One, 2 and 3 weeks after surgery, the tibial callus widths in B<\/jats:styled-content> and C<\/jats:styled-content> group animals were significantly greater than those of group A<\/jats:styled-content> rats (P<\/jats:italic>\u2009<\u20090.05). At 4 weeks the tibial callus width in group C<\/jats:styled-content> animals had decreased, but still differed significantly from that in group A<\/jats:styled-content> rats (P<\/jats:italic>\u2009<\u20090.05). One, 2, 3 and 4 weeks after surgery, the wet weights of B<\/jats:styled-content> and C<\/jats:styled-content> group tibias were significantly greater than those of group A<\/jats:styled-content> (P<\/jats:italic>\u2009<\u20090.05). Hematoxylin\u2013eosin\u2010stained sections showed bony union and increased bone trabecula in B<\/jats:styled-content> and C<\/jats:styled-content> groups and areas with particles positive for alkaline phosphatase staining were more abundant in groups B<\/jats:styled-content> and C<\/jats:styled-content>, especially in group C<\/jats:styled-content>.<\/jats:p><\/jats:sec>Conclusion<\/jats:title>Neural regulation plays an important role in fracture healing. Treatment with BMSCs<\/jats:styled-content> has a positive effect on defective callus in rats that have been subjected to SCT<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/os.12071","type":"journal-article","created":{"date-parts":[[2013,11,20]],"date-time":"2013-11-20T09:05:22Z","timestamp":1384938322000},"page":"280-288","source":"Crossref","is-referenced-by-count":5,"title":["Marrow Stromal Stem Cell Autologous Transplantation in Denervated Fracture Healing: an Experimental Study in Rats"],"prefix":"10.1111","volume":"5","author":[{"given":"Shuan\u2010Hu","family":"Lei","sequence":"first","affiliation":[{"name":"Department of Orthopaedics Second Hospital of Lanzhou University Lanzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Digestion Maternal and Child Health Care Hospital of Gansu Province Lanzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hai\u2010Yuan","family":"Yue","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics Second Hospital of Lanzhou University Lanzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Da\u2010Cheng","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics Second Hospital of Lanzhou University Lanzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cheng\u2010Jun","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics Second Hospital of Lanzhou University Lanzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wen\u2010Jia","family":"Du","sequence":"additional","affiliation":[{"name":"Orthopaedics Key Laboratory of Gansu Province Lanzhou University Lanzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liang\u2010Zeng","family":"Huang","sequence":"additional","affiliation":[{"name":"Orthopaedics Key Laboratory of Gansu Province Lanzhou University Lanzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics Second Hospital of Lanzhou University Lanzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yue\u2010Xiu","family":"Dang","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics Second Hospital of Lanzhou University Lanzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing\u2010Sheng","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics Second Hospital of Lanzhou University Lanzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun\u2010Long","family":"Hao","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics Second Hospital of Lanzhou University Lanzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu\u2010Liang","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics Second Hospital of Lanzhou University Lanzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,11,20]]},"reference":[{"key":"e_1_2_5_2_1","first-page":"177","article-title":"Regional distribution of mast cells and peptide containing nerves in normal and adjuvant arthritic rat synovium","volume":"18","author":"Hukkanen M","year":"1991","journal-title":"J Rheumatol"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/SCS.0b013e31825daab2"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.3109\/17453674.2012.702389"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1206061109"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2982.2011.01728.x"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bone.2012.09.007"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/jor.20807"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/1750-1172-7-S1-S3"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbspin.2012.03.003"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bone.2009.11.029"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.07-098954"},{"key":"e_1_2_5_13_1","first-page":"1524","article-title":"Activation of osteoclasts by RAAS and strategy of target therapy on bone metabolic diseases","volume":"70","author":"Shimizu H","year":"2012","journal-title":"Nihon Rinsho"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0020386"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-8749.2008.03102.x"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcfm.2004.08.001"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1097\/ALN.0b013e3181bf1d93"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1748-1716.1992.tb09382.x"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00223-002-1033-6"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1359\/JBMR.041014"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/jor.1100090504"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0301-472X(00)00482-3"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1097\/00005373-199110000-00026"},{"key":"e_1_2_5_24_1","first-page":"211","article-title":"Healing of experimental fractures in the denervated limbs of the rat","volume":"155","author":"Aro H","year":"1981","journal-title":"Clin Orthop Relat Res"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/304129a0"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/0196-9781(88)90023-X"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00180235"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1097\/00003086-199701000-00043"},{"key":"e_1_2_5_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/S8756-3282(97)00142-7"},{"key":"e_1_2_5_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/jor.1100080311"},{"key":"e_1_2_5_31_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tea.2008.0393"},{"key":"e_1_2_5_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bmt.1703650"},{"key":"e_1_2_5_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(04)16104-7"},{"key":"e_1_2_5_34_1","doi-asserted-by":"publisher","DOI":"10.1634\/stemcells.21-4-437"},{"key":"e_1_2_5_35_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.11.4857"},{"key":"e_1_2_5_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0963-6897(96)00279-5"},{"key":"e_1_2_5_37_1","doi-asserted-by":"publisher","DOI":"10.1634\/stemcells.2007-1028"},{"key":"e_1_2_5_38_1","doi-asserted-by":"publisher","DOI":"10.1089\/cell.2012.0004"},{"key":"e_1_2_5_39_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.22299"},{"key":"e_1_2_5_40_1","doi-asserted-by":"publisher","DOI":"10.3727\/096368911X605510"},{"key":"e_1_2_5_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcyt.2012.09.003"},{"key":"e_1_2_5_42_1","first-page":"381","article-title":"Osteogenesis in transplants of bone marrow cells","volume":"16","author":"Friedenstein AJ","year":"1966","journal-title":"J Embryol Exp Morphol"},{"key":"e_1_2_5_43_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00223-002-2002-9"},{"key":"e_1_2_5_44_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-60327-169-1_12"},{"key":"e_1_2_5_45_1","first-page":"449","article-title":"Immunomagnetic isolation of osteoprogenitors from human bone marrow stroma","volume":"79","author":"Encina NR","year":"1999","journal-title":"Lab Invest"},{"key":"e_1_2_5_46_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02828151"},{"key":"e_1_2_5_47_1","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-140-9-200405040-00013"},{"key":"e_1_2_5_48_1","doi-asserted-by":"publisher","DOI":"10.1159\/000226944"},{"key":"e_1_2_5_49_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.1997.8527"},{"key":"e_1_2_5_50_1","first-page":"459","article-title":"Bromodeoxyuridine increases multipotency of human bone marrow\u2010derived stem cells","volume":"22","author":"Qu TY","year":"2004","journal-title":"Restor Neurol Neurosci"},{"key":"e_1_2_5_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2012.02.020"},{"key":"e_1_2_5_52_1","doi-asserted-by":"publisher","DOI":"10.3109\/17453679608994676"},{"key":"e_1_2_5_53_1","doi-asserted-by":"publisher","DOI":"10.2174\/1566523211313030005"},{"key":"e_1_2_5_54_1","first-page":"569","article-title":"Promotion effect of nuclear factor kappa B p65 on early fracture healing of rat radius by elevating prostaglandins E2 production and regulating inhibitor of DNA binding 2 protein expression","volume":"25","author":"Niu Z","year":"2011","journal-title":"Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi"},{"key":"e_1_2_5_55_1","doi-asserted-by":"publisher","DOI":"10.1097\/BOT.0b013e318274da8b"}],"container-title":["Orthopaedic Surgery"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fos.12071","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/os.12071","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,2]],"date-time":"2023-10-02T05:53:23Z","timestamp":1696226003000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/os.12071"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,11]]},"references-count":54,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2013,11]]}},"alternative-id":["10.1111\/os.12071"],"URL":"https:\/\/doi.org\/10.1111\/os.12071","archive":["Portico"],"relation":{},"ISSN":["1757-7853","1757-7861"],"issn-type":[{"value":"1757-7853","type":"print"},{"value":"1757-7861","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,11]]}}}