{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T13:41:57Z","timestamp":1791294117601,"version":"4.1.0"},"reference-count":20,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2015,6,1]],"date-time":"2015-06-01T00:00:00Z","timestamp":1433116800000},"content-version":"vor","delay-in-days":31,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Orthopaedic Surgery"],"published-print":{"date-parts":[[2015,5]]},"abstract":"Objective<\/jats:title>To explore the toxicity and biological activity of cobalt nanoparticles on the osteoclasts. Analyze the relationship between cobalt nanoparticles and osteolysis.<\/jats:p><\/jats:sec>Methods<\/jats:title>Monocyte\u2010macrophages (RAW<\/jats:styled-content> 264.7) was cultured in vitro, osteoclast\u2010like cells were induced by lipopolysaccharides (LPS<\/jats:styled-content>). After RAW<\/jats:styled-content> 264.7 was induced for 24\u2009h, Methyl Thiazolium Tetrazolium (MTT<\/jats:styled-content>) biological toxicity test of osteoclast\u2010like cell was preceded using Cobalt nanoparticles (set 4 concentrations: 10, 20, 50, 100\u2009\u03bcM) and cobalt chloride (set 4 concentrations: 10, 20, 50, 100\u2009\u03bcM) at 2, 4, 8, 24 and 48\u2009h respectively. The relative expression of mRNA<\/jats:styled-content> of CA II<\/jats:styled-content> and C<\/jats:styled-content>at K<\/jats:styled-content> after RAW<\/jats:styled-content> 264.7 induction was determined by Q<\/jats:styled-content>\u2010PCR<\/jats:styled-content>.<\/jats:p><\/jats:sec>Results<\/jats:title>mRNA<\/jats:styled-content> relative expression of CA II<\/jats:styled-content>, C<\/jats:styled-content>at K<\/jats:styled-content> were reduced at multiple concentrations both cobalt nanoparticles and cobalt chloride, and was time and concentration dependent, cobalt nanoparticles are more significant than cobalt chloride group. But when the cobalt nanoparticles concentration is in 10\u201350\u2009\u03bcM, the mRNA<\/jats:styled-content> relative expression of CA II<\/jats:styled-content>, C<\/jats:styled-content>at K<\/jats:styled-content> increased.<\/jats:p><\/jats:sec>Conclusion<\/jats:title>Cobalt nanoparticles have biological toxicity. At multiple concentrations, the differentiation and proliferation of osteoclasts was inhibited, but when the concentration of cobalt nanoparticles is in 10\u201350\u2009\u03bcM, it has been strengthened.<\/jats:p><\/jats:sec>","DOI":"10.1111\/os.12180","type":"journal-article","created":{"date-parts":[[2015,6,1]],"date-time":"2015-06-01T06:44:26Z","timestamp":1433141066000},"page":"168-173","source":"Crossref","is-referenced-by-count":22,"title":["Toxicity and Bioactivity of Cobalt Nanoparticles on the Monocytes"],"prefix":"10.1111","volume":"7","author":[{"given":"Ya\u2010ke","family":"Liu","sequence":"first","affiliation":[{"name":"Institute of Orthopaedic Surgery Affiliated Hospital of NanTong University Nantong Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Ye","sequence":"additional","affiliation":[{"name":"Institute of Orthopaedic Surgery Affiliated Hospital of NanTong University Nantong Jiangsu 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