{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T00:18:36Z","timestamp":1790468316708,"version":"4.1.0"},"reference-count":33,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2018,1,30]],"date-time":"2018-01-30T00:00:00Z","timestamp":1517270400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Fujian Youth Talents General Training Program","award":["2014-ZQN-JC-19"],"award-info":[{"award-number":["2014-ZQN-JC-19"]}]},{"name":"Medical Innovation Project of Fujian Province","award":["2014-CX-23"],"award-info":[{"award-number":["2014-CX-23"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Orthopaedic Surgery"],"published-print":{"date-parts":[[2018,2]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>To explore the diagnostic efficiency of DNA\u2010based and RNA\u2010based quantitative polymerase chain reaction (qPCR) analyses for periprosthetic joint infection (PJI).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>To determine the detection limit of DNA\u2010based and RNA\u2010based qPCR<jats:italic>in vitro<\/jats:italic>,<jats:styled-content style=\"fixed-case\"><jats:italic>Staphylococcus aureus<\/jats:italic><\/jats:styled-content>and<jats:styled-content style=\"fixed-case\"><jats:italic>Escherichia coli<\/jats:italic><\/jats:styled-content>strains were added to sterile synovial fluid obtained from a patient with knee osteoarthritis. Serial dilutions of samples were analyzed by DNA\u2010based and RNA\u2010based qPCR. Clinically, patients who were suspected of having PJI and eventually underwent revision arthroplasty in our hospital from July 2014 to December 2016 were screened. Preoperative puncture or intraoperative collection was performed on patients who met the inclusion and exclusion criteria to obtain synovial fluid. DNA\u2010based and RNA\u2010based PCR analyses and culture were performed on each synovial fluid sample. The patients\u2019 demographic characteristics, medical history, and laboratory test results were recorded. The diagnostic efficiency of both PCR assays was compared with culture methods.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The<jats:italic>in\u2009vitro<\/jats:italic>analysis demonstrated that DNA\u2010based qPCR assay was highly sensitive, with the detection limit being 1200 colony forming units (CFU)\/mL of<jats:styled-content style=\"fixed-case\"><jats:italic>S. aureus<\/jats:italic><\/jats:styled-content>and 3200\u2009CFU\/mL of<jats:styled-content style=\"fixed-case\"><jats:italic>E. coli<\/jats:italic><\/jats:styled-content>. Meanwhile, The RNA\u2010based qPCR assay could detect 2300\u2009CFU\/mL of<jats:styled-content style=\"fixed-case\"><jats:italic>S. aureus<\/jats:italic><\/jats:styled-content>and 11\u2009000\u2009CFU\/mL of<jats:styled-content style=\"fixed-case\"><jats:italic>E. coli<\/jats:italic><\/jats:styled-content>. Clinically, the sensitivity, specificity, and accuracy were 65.7%, 100%, and 81.6%, respectively, for the culture method; 81.5%, 84.8%, and 83.1%, respectively, for DNA\u2010based qPCR; and 73.6%, 100%, and 85.9%, respectively, for RNA\u2010based qPCR.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>DNA\u2010based qPCR could detect suspected PJI with high sensitivity after antibiotic therapy. RNA\u2010based qPCR could reduce the false positive rates of DNA\u2010based assays. qPCR\u2010based methods could improve the efficiency of PJI diagnosis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/os.12359","type":"journal-article","created":{"date-parts":[[2018,1,31]],"date-time":"2018-01-31T04:36:39Z","timestamp":1517373399000},"page":"40-46","source":"Crossref","is-referenced-by-count":20,"title":["Detecting the Presence of Bacterial DNA and RNA by Polymerase Chain Reaction to Diagnose Suspected Periprosthetic Joint Infection after Antibiotic Therapy"],"prefix":"10.1111","volume":"10","author":[{"given":"Xin\u2010yu","family":"Fang","sequence":"first","affiliation":[{"name":"Department of Orthopaedic Surgery The First Affiliated Hospital of Fujian Medical University Fuzhou China"},{"name":"Department of Orthopaedic Surgery The Third Affiliated Hospital of Fujian Medical University Fuzhou 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