{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:19:32Z","timestamp":1787015972276,"version":"build-2736575974"},"reference-count":28,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2018,11,25]],"date-time":"2018-11-25T00:00:00Z","timestamp":1543104000000},"content-version":"vor","delay-in-days":24,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004731","name":"Natural Science Foundation of Zhejiang Province","doi-asserted-by":"publisher","award":["LZ15H060002"],"award-info":[{"award-number":["LZ15H060002"]}],"id":[{"id":"10.13039\/501100004731","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Orthopaedic Surgery"],"published-print":{"date-parts":[[2018,11]]},"abstract":"<jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>Turnover of cartilage endplate extracellular matrix (ECM) may play an important role in disc degeneration and low back pain (LBP). However, the expression pattern of pro\u2010inflammatory factors, matrix metalloproteinases (MMP), and tissue inhibitors of metalloproteinases (TIMP) in the cartilage endplates (CEP) of intervertebral discs (IVD) is not understood. We aimed to examine the transcriptional levels of MMP, TIMP, and interleukins (IL), and the correlations between them.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Thirty degenerated cartilage endplate samples from patients with LBP who underwent lumbar fusion surgery were included in the degenerated group. Ten patients without LBP history who underwent lumbar surgery because of vertebral burst fractures were included in the control group. The degenerative severity of the samples was evaluated by MRI, and hematoxylin\u2013eosin and safranin O\u2010fast green (SO\u2010FG) staining. Real\u2010time polymerase chain reaction (RT\u2010PCR) was used to detect the mRNA levels of MMP\u20101, MMP\u20103, MMP\u20109, MMP\u201013, TIMP\u20101, TIMP\u20102, TIMP\u20103, IL\u20101\u03b1, IL\u20101\u03b2, and IL\u20106. The correlations between the levels of these genes were tested using Spearman\u2019s rho test.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Hematoxylin\u2013eosin and SO\u2010FG staining confirmed a decrease in cell number and proteoglycans in the degenerated cartilage endplate. MRI showed significant signal changes in degenerated cartilage endplates. Patients in the degenerated group showed a higher rate of endplate Modic changes when compared with the control group. MMP\u20103, MMP\u20109, TIMP\u20103, IL\u20101\u03b1, and IL\u20101\u03b2 were elevated with statistical significance, while MMP\u20101, MMP\u201013, TIMP\u20101, TIMP\u20102, and IL\u20106 were changed without statistical significance or remained unchanged. Expression of MMP\u20103 was positively correlated with IL\u20101\u03b1 (Spearman coefficient, 0.486;\n                      <jats:italic>P\u2009&lt;<\/jats:italic>\n                      0.05); expression of TIMP\u20103 was positively correlated with MMP\u20109, IL\u20101\u03b1, and IL\u20101\u03b2 (Spearman coefficient, 0.577, 0.407, and 0.571, respectively;\n                      <jats:italic>P\u2009&lt;<\/jats:italic>\n                      0.05).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>MMP\u20103, MMP\u20109, TIMP\u20103, IL\u20101\u03b1, and IL\u20101\u03b2 may play a role in the process of cartilage endplate degeneration. MMP\u20103 may be regulated by IL\u20101\u03b1, and TIMP\u20103 might be associated with MMP\u20109 and regulated by IL\u20101\u03b1 and IL\u20101\u03b2.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/os.12409","type":"journal-article","created":{"date-parts":[[2018,11,26]],"date-time":"2018-11-26T02:30:26Z","timestamp":1543199426000},"page":"306-311","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":38,"title":["Expression of Matrix Metalloproteinases, Tissue Inhibitors of Metalloproteinases, and Interleukins in Vertebral Cartilage Endplate"],"prefix":"10.1111","volume":"10","author":[{"given":"Jian\u2010feng","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Orthopaedics, Sir Run Run Shaw Hospital Medical College of Zhejiang University  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gang\u2010liang","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics, Sir Run Run Shaw Hospital Medical College of Zhejiang University  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhi\u2010jie","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics, Sir Run Run Shaw Hospital Medical College of Zhejiang University  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiang\u2010qian","family":"Fang","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics, Sir Run Run Shaw Hospital Medical College of Zhejiang University  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuai","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics, Sir Run Run Shaw Hospital Medical College of Zhejiang University  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shun\u2010wu","family":"Fan","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics, Sir Run Run Shaw Hospital Medical College of Zhejiang University  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,11,25]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM200102013440508"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.2106\/JBJS.I.01568"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pain.2007.01.010"},{"key":"e_1_2_6_5_1","first-page":"211","article-title":"Volvo award winner in basic science. 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An experimental study using hydrogen washout technique","volume":"1981","author":"Ogata K","year":"1980","journal-title":"Spine (Phila Pa 1976)"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1097\/BRS.0000000000000372"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/ar3764"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1097\/BRS.0000000000000362"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12192-015-0600-5"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1185\/030079907X223486"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj1950159"},{"key":"e_1_2_6_12_1","article-title":"Selection of suitable reference genes for normalization of quantitative real\u2010time polymerase chain reaction in human cartilage endplate of the lumbar spine","volume":"9","author":"Zhou ZJ","year":"2014","journal-title":"PLoS One"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.31.21491"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.2741\/1817"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1615\/CritRevEukarGeneExpr.v6.i4.40"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm763"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780380205"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-4838(99)00279-4"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(95)48032-3"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI114215"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M312123200"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj2940761"},{"key":"e_1_2_6_23_1","first-page":"870","article-title":"Immunohistological analysis of cytokine expression in human osteoarthritic and healthy cartilage","volume":"26","author":"Moos V","year":"1999","journal-title":"J Rheumatol"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.2741\/Martel"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780400604"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.12.7727"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12272-016-0855-0"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0945-053X(99)00021-9"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0049-0172(96)80036-9"}],"container-title":["Orthopaedic Surgery"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fos.12409","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/os.12409","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,15]],"date-time":"2023-09-15T10:35:01Z","timestamp":1694774101000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/os.12409"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,11]]},"references-count":28,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2018,11]]}},"alternative-id":["10.1111\/os.12409"],"URL":"https:\/\/doi.org\/10.1111\/os.12409","archive":["Portico"],"relation":{},"ISSN":["1757-7853","1757-7861"],"issn-type":[{"value":"1757-7853","type":"print"},{"value":"1757-7861","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,11]]},"assertion":[{"value":"2017-05-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-10-30","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-11-25","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}