{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,6]],"date-time":"2025-11-06T16:02:04Z","timestamp":1762444924999,"version":"3.40.5"},"reference-count":24,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2019,6,26]],"date-time":"2019-06-26T00:00:00Z","timestamp":1561507200000},"content-version":"vor","delay-in-days":25,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Fujian Commission of Finance Foundation Project","award":["2018B022","BPB-LJH2015-2"],"award-info":[{"award-number":["2018B022","BPB-LJH2015-2"]}]},{"name":"Key Clinical Specialty Discipline Construction Program of Fujian P.R.C"},{"name":"Proper Technology Promotion Project of Fujian Health and Family Planning in Rural and Urban Community","award":["2017-017"],"award-info":[{"award-number":["2017-017"]}]},{"name":"Startup Fund for scientific research\uff0cFujian Medical University","award":["2017XQ1075"],"award-info":[{"award-number":["2017XQ1075"]}]},{"name":"Youth and Middle-aged Backbone Culture Project of Fujian Health and Family Planning Commission","award":["2017-ZQN-48"],"award-info":[{"award-number":["2017-ZQN-48"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Orthopaedic Surgery"],"published-print":{"date-parts":[[2019,6]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>The present study aimed to identify the relationship of \u03b1\u20102\u2010macroglobulin and microvascular vessel pathology with steroid\u2010induced femoral head necrosis in the Southeast Chinese population.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>This study enrolled 40 patients diagnosed with steroid\u2010induced necrosis of the femoral head. Patients had various stages of femoral head necrosis. The differential expression of serum proteins and mRNA from patients with steroid\u2010induced necrosis of the femoral head (SINFH) and healthy volunteers was analyzed by western blot and quantitative polymerase chain reaction (QT\u2010PCR). The pathological change in osteocyte necrosis was indicated by hematoxylin and eosin stain and immunohistochemistry.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Hematoxylin and eosin stain showed histopathology changes in the necrotic area of patients with steroid\u2010induced INFH: bone trabeculae were fewer and thinner, became broken, fragmented and structurally disordered; intraosseous adipose cells became enlarged; the arrangement of the osteoblasts became irregular; and vacant bone lacunae increased. QT\u2010PCR showed significantly lower levels of \u03b1\u20102\u2010macroglobulin in the serum of patients with SINFH than in controls (<jats:italic>P<\/jats:italic> &lt; 0.05). Immunohistochemical staining and western blotting demonstrated that the expression of \u03b1\u20102\u2010macroglobulin was significantly decreased in the necrotic area of SINFH patients (<jats:italic>P<\/jats:italic> &lt; 0.05).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The \u03b1\u20102\u2010macroglobulin may be associated with the pathology of SINFH. The multiple pathological reactions occur in SINFH and \u03b1\u20102\u2010macroglobulin may serve as a potential biomarker for the diagnosis of SINFH or a promising therapeutic target.<\/jats:p><\/jats:sec>","DOI":"10.1111\/os.12492","type":"journal-article","created":{"date-parts":[[2019,6,27]],"date-time":"2019-06-27T04:36:36Z","timestamp":1561610196000},"page":"481-486","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Relationship of \u03b12\u2010Macroglobulin with Steroid\u2010Induced Femoral Head Necrosis: A Chinese Population\u2010Based Association Study in Southeast China"],"prefix":"10.1111","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9892-8557","authenticated-orcid":false,"given":"Shan\u2010hong","family":"Fang","sequence":"first","affiliation":[{"name":"Department of Orthopaedics First Affiliated Hospital of Fujian Medical University  Fuzhou City China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yong\u2010feng","family":"Li","sequence":"additional","affiliation":[{"name":"Fujian Medical University  Fuzhou City China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jia\u2010run","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics The Hospital of Changle District  Fuzhou City China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peng","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics First Affiliated Hospital of Fujian Medical University  Fuzhou City 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