{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:20:00Z","timestamp":1787016000311,"version":"build-2736575974"},"reference-count":42,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2019,10,29]],"date-time":"2019-10-29T00:00:00Z","timestamp":1572307200000},"content-version":"vor","delay-in-days":28,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81573754; 81803902; 81973884"],"award-info":[{"award-number":["81573754; 81803902; 81973884"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Orthopaedic Surgery"],"published-print":{"date-parts":[[2019,10]]},"abstract":"<jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>For the purpose of providing evidence for the treatment of osteoporosis and osteopenia, this study retrospectively identified succinylation\u2010modified sites and proteins in postmenopausal women, and bioinformatics analysis were performed.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>From January 2016 to June 2018, a total of 30 postmenopausal women aged from 55 to 70\u2009years old were assigned to three groups: 10 cases with osteoporosis; 10 cases with osteopenia; and 10 cases with normal bone mass. Subsequently, the serum samples were collected from all cases for succinyl\u2010proteome. Measures comprised label\u2010free quantitative analysis, succinylation enrichment techniques, the liquid chromatograph\u2013mass spectrometer\/mass spectrometer (LC\u2010MS\/MS) methods, and bioinformatics.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>A total of 113 succinylation sites on 35 proteins were identified based on quantitative information. The variation of the different multiple folds were more than 1.2 times as a significant increase for up\u2010regulated and less than 1\/1.2 times as a significant decrease for down\u2010regulated. Among the quantified succinylation sites, 66 were up\u2010regulated and 11 down\u2010regulated in the Osteopenia\/Normal comparison group, 24 were up\u2010regulated and 44 down\u2010regulated in the Osteoporosis\/Osteopenia comparison group, 45 were up\u2010regulated and 32 down\u2010regulated in the Osteoporosis\/Normal comparison group. Among the quantified succinylation proteins, 24 were up\u2010regulated and 7 down\u2010regulated in the Osteopenia\/Normal comparison group, 15 were up\u2010regulated and 20 down\u2010regulated in the Osteoporosis\/Osteopenia comparison group, 20 were up\u2010regulated and 17 down\u2010regulated in the Osteoporosis\/Normal comparison group. The percentage of proteins differed in immune response, signaling pathway, proteolysis, lymphocyte, leukocyte, and cell activation. Four differentially expressed proteins (apolipoprotein A\u2010I, apolipoprotein A\u2010II, hemoglobin subunit alpha, and haptoglobin) contained quantitative information; they were mediated with receptors, factors, mechanisms, that related to bone metabolism. Hemoglobin subunit alpha was screened for diagnosis of osteopenia.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The succinyl\u2010proteome experimental data indicated that apolipoprotein A\u2010I, apolipoprotein A\u2010II, hemoglobin subunit alpha, and haptoglobin were valuable for diagnosis and treatment in postmenopausal women with osteoporosis and osteopenia.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/os.12519","type":"journal-article","created":{"date-parts":[[2019,10,29]],"date-time":"2019-10-29T20:57:43Z","timestamp":1572382663000},"page":"784-793","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Discovery and Identification of Serum Succinyl\u2010Proteome for Postmenopausal Women with Osteoporosis and Osteopenia"],"prefix":"10.1111","volume":"11","author":[{"given":"Li\u2010li","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Pathology The Second Affiliated Hospital Zhejiang University School of Medicine  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chun\u2010wen","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Diagnostics of Traditional Chinese Medicine, College of Basic Medical Science Zhejiang Chinese Medical University  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7109-3174","authenticated-orcid":false,"given":"Kang","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Osteology The Second Affiliated Hospital of Zhejiang Chinese Medical University  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhong","family":"Liu","sequence":"additional","affiliation":[{"name":"The Second Clinical Medical College Zhejiang Chinese Medical University  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo\u2010cheng","family":"Liang","sequence":"additional","affiliation":[{"name":"Department of Osteology The Second Affiliated Hospital of Zhejiang Chinese Medical University  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7759-1342","authenticated-orcid":false,"given":"Yi\u2010ran","family":"Yang","sequence":"additional","affiliation":[{"name":"The Second Clinical Medical College Zhejiang Chinese Medical University  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao\u2010lin","family":"Shi","sequence":"additional","affiliation":[{"name":"Department of Osteology The Second Affiliated Hospital of Zhejiang Chinese Medical University  Hangzhou 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