{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:17:28Z","timestamp":1787015848740,"version":"build-2736575974"},"reference-count":43,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,1,8]],"date-time":"2020-01-08T00:00:00Z","timestamp":1578441600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004763","name":"Natural Science Foundation of Inner Mongolia","doi-asserted-by":"publisher","award":["201703122"],"award-info":[{"award-number":["201703122"]}],"id":[{"id":"10.13039\/501100004763","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Orthopaedic Surgery"],"published-print":{"date-parts":[[2020,2]]},"abstract":"<jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To investigate whether miR\u2010141 and the sex determination region of Y chromosome box 11 (SOX11) play roles in steroid\u2010induced avascular necrosis of the femoral head (SANFH), and to explore whether miR\u2010141 could target SOX11 to influence the proliferation of bone marrow mesenchymal stem cells (BMSC).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Bone marrow mesenchymal stem cells (BMSC) were isolated and cultured from 4\u2010week\u2010old Sprague Dawley rats. A flow cytometry assay was performed to identify BMSC. BMSC were divided into two groups: a control group and a dexamethasone (DEX) group. BMSC were transfected by miR\u2010141 mimic, miR\u2010141 inhibitor, and SOX11. Real\u2010time polymerase chain reaction (PCR) assay was performed to investigate the mRNA expression of miR\u2010141 and SOX11. The results were used to determine the effect of transfection and to verify the expression in each group and the association between miR\u2010141 and SOX11. Luciferase reporter assay revealed the targeted binding site between miR\u2010141 and the 3\u2032\u2010untranslated region of SOX11 mRNA. MTT assays were performed to investigate the proliferation of BMSC in the miR\u2010141 mimic, miR\u2010141 inhibitor, and SOX11 groups.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Result<\/jats:title>\n                    <jats:p>\n                      The results of the flow cytometry assay suggested that cells were positive for CD29 and CD90 while negative for CD45. This meant that the isolated and cultured cells were not hematopoietic stem cells. In addition, cell transfection was successful based on the expression of miR\u2010141 and SOX11. According to the results of real\u2010time PCR assay, the mRNA expression of miR\u2010141 in SANFH was upregulated (4.117\u2009\u00b1\u20090.042\n                      <jats:italic>vs<\/jats:italic>\n                      1\u2009\u00b1\u20090.027,\n                      <jats:italic>P<\/jats:italic>\n                      &lt;\u20090.001), while SOX11 was downregulated (0.611\u2009\u00b1\u20090.055\n                      <jats:italic>vs<\/jats:italic>\n                      1\u2009\u00b1\u20090.027,\n                      <jats:italic>P<\/jats:italic>\n                      &lt;\u20090.001) compared with the control group. Based on the results of the luciferase experiment, MiR\u2010141 could directly target the expression of SOX11. Inhibition of miR\u2010141 could upregulate the expression of SOX11 (2.623\u2009\u00b1\u20090.220\n                      <jats:italic>vs<\/jats:italic>\n                      1\u2009\u00b1\u20090.095,\n                      <jats:italic>P<\/jats:italic>\n                      &lt;\u20090.001) according to the results of a real\u2010time PCR assay. MiR\u2010141 inhibited the proliferation of BMSC (0.618\u2009\u00b1\u20090.092\n                      <jats:italic>vs<\/jats:italic>\n                      1.004\u2009\u00b1\u20090.082,\n                      <jats:italic>P<\/jats:italic>\n                      &lt;\u20090.001), while suppression of miR\u2010141 increased the proliferation of BMSC (0.960\u2009\u00b1\u20090.095\n                      <jats:italic>vs<\/jats:italic>\n                      0.742\u2009\u00b1\u20090.091,\n                      <jats:italic>P<\/jats:italic>\n                      &lt;\u20090.001). Furthermore, according to the results of the MTT assay, SOX11 promoted the proliferation of BMSC (1.064\u2009\u00b1\u20090.093\n                      <jats:italic>vs<\/jats:italic>\n                      0.747\u2009\u00b1\u20090.090,\n                      <jats:italic>P<\/jats:italic>\n                      &lt;\u20090.001).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>MiR\u2010141 inhibited the proliferation of BMSC in SANFH by targeting SOX11. Inhibition of miR\u2010141 upregulated the expression of SOX11 and promoted the proliferation of BMSC. MiR\u2010141 and SOX11 could be new targets for investigating the mechanism of SANFH.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/os.12603","type":"journal-article","created":{"date-parts":[[2020,1,8]],"date-time":"2020-01-08T21:17:00Z","timestamp":1578518220000},"page":"277-285","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Influence of MicroRNA\u2010141 on Inhibition of the Proliferation of Bone Marrow Mesenchymal Stem Cells in Steroid\u2010Induced Osteonecrosis\n                    <i>via<\/i>\n                    SOX11"],"prefix":"10.1111","volume":"12","author":[{"given":"Chen\u2010yang","family":"Meng","sequence":"first","affiliation":[{"name":"Orthopedics Department Second Affiliated Hospital of Inner Mongolia Medical University  Hohhot China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fei","family":"Xue","sequence":"additional","affiliation":[{"name":"Orthopedics Department Second Affiliated Hospital of Inner Mongolia Medical University  Hohhot China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhen\u2010qun","family":"Zhao","sequence":"additional","affiliation":[{"name":"Orthopedics Department Second Affiliated Hospital of Inner Mongolia Medical University  Hohhot China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ting","family":"Hao","sequence":"additional","affiliation":[{"name":"Orthopedics Department Second Affiliated Hospital of Inner Mongolia Medical University  Hohhot China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shi\u2010bing","family":"Guo","sequence":"additional","affiliation":[{"name":"Orthopedics Department Inner Mongolia Institute of Orthopedics  Hohhot China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7913-2011","authenticated-orcid":false,"given":"Wei","family":"Feng","sequence":"additional","affiliation":[{"name":"Orthopedics Department Second Affiliated Hospital of Inner Mongolia Medical University  Hohhot China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,1,8]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/rheumatology\/ker277"},{"key":"e_1_2_6_3_1","first-page":"153","article-title":"Aseptic bone necrosis in patients on glucocorticoid replacement therapy","volume":"39","author":"Vreden SG","year":"1991","journal-title":"Neth J Med"},{"key":"e_1_2_6_4_1","first-page":"1030","article-title":"Effects of antler powder on treatment of corticosteroid\u2010induced avascular necrosis of the femoral head in rats","volume":"346","author":"Baktursun P","year":"2011","journal-title":"J Clin Rehabil Tissue Eng Res"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0030-5898(20)30431-4"},{"key":"e_1_2_6_6_1","first-page":"490","article-title":"Osteonecrosis of the femoral head: lipotoxicity exacerbation in MSC and modifications of the bone marrow fluid","volume":"158","author":"Gillet C","year":"2017","journal-title":"Endocrinology"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10735-014-9571-6"},{"key":"e_1_2_6_8_1","first-page":"2028","article-title":"Dexamethasone\u2010induced production of reactive oxygen species promotes apoptosis via endoplasmic reticulum stress and autophagy in MC3T3\u2010E1 cells","volume":"41","author":"Liu W","year":"2018","journal-title":"Int J Mol Med"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0042-117721"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2005.01.117"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.16.8.4128"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1097\/00007890-196803000-00009"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.3109\/s10165-008-0023-6"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/jor.1100090504"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(96)00240-2"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2015.3472"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.29161"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.29166"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1177\/1535370214566565"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00198-015-3308-6"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/jor.20078"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(93)90529-Y"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng1590"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.2806"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrg1379"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1530\/JME-14-0314"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/cdd.2015.99"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.3892\/ijmm.2015.2273"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2014.298"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2015.01.007"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.3892\/ijo.2014.2698"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcla.22293"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms18061208"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tea.2011.0432"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.steroids.2011.10.017"},{"key":"e_1_2_6_36_1","first-page":"1240","article-title":"Model establishment, MRI and pathological features of early steroid\u2010induced avascular necrosis of femoral head in rabbit","volume":"29","author":"Zhang L","year":"2015","journal-title":"Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.4238\/gmr.15017529"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.3892\/etm.2016.3991"},{"key":"e_1_2_6_39_1","first-page":"645","article-title":"A regulatory miRNA\u2010mRNA network is associated with tissue repair induced by mesenchymal stromal cells in acute kidney injury","volume":"7","author":"Almeida DC","year":"2016","journal-title":"Front Immunol"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.133314"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.3892\/or.2016.5292"},{"key":"e_1_2_6_42_1","first-page":"4345","article-title":"miR\u2010141 inhibits proliferation and migration of colorectal cancer SW480 cells","volume":"37","author":"Long ZH","year":"2017","journal-title":"Anticancer Res"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13046-017-0645-7"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tea.2010.0085"}],"container-title":["Orthopaedic Surgery"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fos.12603","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/os.12603","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/os.12603","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/os.12603","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,2]],"date-time":"2023-09-02T19:34:05Z","timestamp":1693683245000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/os.12603"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,1,8]]},"references-count":43,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2020,2]]}},"alternative-id":["10.1111\/os.12603"],"URL":"https:\/\/doi.org\/10.1111\/os.12603","archive":["Portico"],"relation":{},"ISSN":["1757-7853","1757-7861"],"issn-type":[{"value":"1757-7853","type":"print"},{"value":"1757-7861","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,1,8]]},"assertion":[{"value":"2019-09-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-12-05","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-01-08","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}