{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T22:38:06Z","timestamp":1768343886885,"version":"3.49.0"},"reference-count":34,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2020,2,16]],"date-time":"2020-02-16T00:00:00Z","timestamp":1581811200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81760391\u300181560349\u300181460331"],"award-info":[{"award-number":["81760391\u300181560349\u300181460331"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Orthopaedic Surgery"],"published-print":{"date-parts":[[2020,4]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>To study the role of primary cilia formation disorder and osteoblasts autophagy in the pathogenesis of steroid\u2010induced avascular necrosis of the femoral head (SANFH).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Osteoblasts were isolated from rabbit bones and treated with 1\u2009\u03bcM Methylprednisolone for 0, 12, 24, 48, and 72\u2009h. The Beclin1, MAP1LC3, Atg\u20105, Atg\u201012, IFT20 and OFD1 mRNAs and proteins were detected by PCR and Western blotting, and their correlation was statistically analyzed. The lengths of osteoblast cilia were measured under a laser confocal microscope, and the autophagy flux was tracked by transfecting the osteoblasts with GFP\u2010RFP\u2010LC3 lentivirus.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Methylprednisolone significantly upregulated Beclin1, MAP1LC3, Atg\u20105, Atg\u201012 and OFD1 mRNAs and proteins in a time\u2010dependent manner, and decreased that of IFT20 (<jats:italic>P<\/jats:italic> &lt;\u20090.05). In addition, the autophagy flux in the osteoblasts also increased and the ciliary length decreased in a time\u2010dependent manner after Methylprednisolone treatment. The length of the cilia were 5.46 \u00b1\u20090.11\u2009um at 0\u2009h, 4.08 \u00b1\u20090.09\u2009um at 12\u2009h, 3.07 \u00b1\u20090.07\u2009um at 24 h, 2.31 \u00b1\u20090.10\u2009um at 48 h, and finally 1.15 \u00b1\u20090.04\u2009um at 72\u2009h. Methylprednisolone treatment also affects primary cilium numbers in cultures, for 0 to 72\u2009h. The autophagy regulatory genes, Beclin1, MAP1LC3, Atg\u20105 and Atg\u201012, were found to be negatively correlated with IFT20, with an average correlation coefficient of \u22120.81. A negative correlation was also found between OFD1 and IFT20, with an average correlation coefficient of \u22120.53.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Methylprednisolone inhibits primary cilia formation and promotes autophagy, which could be the pathological basis of SANFH. The exact regulatory mechanism needs to be further studied <jats:italic>in vivo<\/jats:italic>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/os.12630","type":"journal-article","created":{"date-parts":[[2020,2,17]],"date-time":"2020-02-17T06:45:30Z","timestamp":1581921930000},"page":"645-652","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Mechanism of Methylprednisolone\u2010Induced Primary Cilia Formation Disorder and Autophagy in Osteoblasts"],"prefix":"10.1111","volume":"12","author":[{"given":"Zhen\u2010qun","family":"Zhao","sequence":"first","affiliation":[{"name":"Orthopedics Department Second Affiliated Hospital of Harbin Medical University  Harbin China"},{"name":"Pediatric Orthopedics Department Second Affiliated Hospital of Inner Mongolia Medical University  Inner Mongolia 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