{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T21:24:45Z","timestamp":1790717085914,"version":"4.1.0"},"reference-count":45,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2022,8,10]],"date-time":"2022-08-10T00:00:00Z","timestamp":1660089600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81871124"],"award-info":[{"award-number":["81871124"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Orthopaedic Surgery"],"published-print":{"date-parts":[[2022,9]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>To investigate the effect of intermittent parathyroid hormone (PTH) on gut microbiota (GM) in ovariectomized (OVX) osteoporotic rats.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Thirty female Sprague\u2013Dawley rats were divided into three groups: sham\u2010operation (SHAM) group, OVX group and PTH treatment group. After 3\u00a0months of treatment, the femurs, serum and feces were acquired for micro\u2010CT, biochemical analysis and 16S rRNA sequencing, respectively. For 16S rRNA sequencing, after raw reads filtrated and chimera sequences removed, the clean reads were obtained. According to these clean reads, the operational taxonomic units (OTUs) were clustered. Venn diagram analysis was conducted to explore common and unique GM among the three groups. The \u03b1\u2010diversity analysis including Shannon and Simpson indexes were used to evaluate the richness and diversity of the GM. The \u03b2\u2010diversity analysis was performed to estimate the structure of GM. The metabolic function was predicted by Tax4Fun analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>With micro\u2010CT and biochemical analysis, significant improvements were found in the PTH group compared with the OVX group. In Venn diagram analysis, more unique OTUs were found in the SHAM and PTH groups than the OVX group. According to the rank abundance curve, the SHAM and PTH groups had similar richness and evenness, which were higher than the OVX group. Simpson and Shannon indexes were higher in the SHAM and PTH groups compared with the OVX group, indicating that the SHAM and PTH groups had higher microbiota complexity than the OVX group. In \u03b2\u2010diversity analysis, apparent separation was found in the OVX group from the PTH and SHAM groups, which suggested that osteoporosis is the critical factor influencing the GM composition and PTH treatment and can restore the structure of GM. Compared with the OVX group, treatment with PTH increased the abundances of GM which were reported to increase bone mass, such as<jats:italic>Lactobacillus_reuteri<\/jats:italic>,<jats:italic>Muribaculaceae<\/jats:italic>,<jats:italic>Ruminococcaceae<\/jats:italic>, and<jats:italic>Clostridia<\/jats:italic>, and inhibited the relative abundance of<jats:italic>Rikenellaceae<\/jats:italic>, which was reported to be potentially related to osteoporosis. GM function analysis showed that PTH could promote butyrate synthesis. In Tax4Fun analysis, the function of butanoate metabolism is more vital in the PTH group than the OVX and SHAM groups, suggesting PTH treatment could regulate microbial metabolic function, including butanoate metabolism.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Intermittent PTH can interact with GM through increasing the abundance of probiotics and reducing the abundance of the pathogenic bacteria to enhance the bone mass.<\/jats:p><\/jats:sec>","DOI":"10.1111\/os.13419","type":"journal-article","created":{"date-parts":[[2022,8,10]],"date-time":"2022-08-10T09:29:33Z","timestamp":1660123773000},"page":"2330-2338","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Intermittent Parathyroid Hormone Alters Gut Microbiota in Ovariectomized Osteoporotic Rats"],"prefix":"10.1111","volume":"14","author":[{"given":"Jiaming","family":"Zhou","sequence":"first","affiliation":[{"name":"Department of Orthopaedic Surgery Tianjin Medical University General Hospital Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery Tianjin Medical University General Hospital Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery Tianjin Medical University General Hospital Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xing","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery Tianjin Medical University General Hospital Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pengguo","family":"Gou","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery The Affiliated People's Hospital of Shanxi Medical University Taiyuan 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