{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:17:35Z","timestamp":1787015855550,"version":"build-2736575974"},"reference-count":39,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2023,1,31]],"date-time":"2023-01-31T00:00:00Z","timestamp":1675123200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100021171","name":"Guangdong Basic and Applied Basic Research Foundation","doi-asserted-by":"publisher","award":["2021A1515110885"],"award-info":[{"award-number":["2021A1515110885"]}],"id":[{"id":"10.13039\/501100021171","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82072453"],"award-info":[{"award-number":["82072453"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Orthopaedic Surgery"],"published-print":{"date-parts":[[2023,3]]},"abstract":"<jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>Wear particles induce inflammation and the further osteolysis around the prosthesis, has been proven to be the main cause of aseptic hip joint loosening. In this research, we aimed to clarify whether human umbilical cord mesenchymal stem cells (HUCMSCs) could inhibit the titanium particles\u2010induced osteolysis and shed light upon its mechanism.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      The expression of chemokine (C\u2010C motif) ligand 2 (CCL2), chemokine (C\u2010C motif) ligand 3 (CCL3) and chemokine (C\u2010C motif) ligand 5 (CCL5) were examinjed in clinical specimens of aseptic hip prosthesis loosening patients. Local injection of lentivirus that knocked down CCL2 or CCL3 in a cranial osteolysis mice model were used to exam the effect of CCL2 and CCL3 on titanium particles\u2010induced osteolysis\n                      <jats:italic>in vivo<\/jats:italic>\n                      . Transwell assay was used to examine the effect of CCL2 and CCL3 on titanium particles\u2010induced activation of macrophage\n                      <jats:italic>in vitro<\/jats:italic>\n                      . Furthermore, the therapeutic effect of HUCMSCs, and exosomes from HUCMSCs were also examed\n                      <jats:italic>in vivo and vitro<\/jats:italic>\n                      . Immunohistochemical and real\u2010time PCR were used to examine the expression of relative pathways. Analysis of variance (ANOVA) and Student\u2013Newman\u2013Keuls\n                      <jats:italic>post hoc t<\/jats:italic>\n                      test were used to analyze the results and determine the statistical significance of the differences.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Results showed that titanium particles caused the osteolysis at the mice cranial\n                      <jats:italic>in vivo<\/jats:italic>\n                      and a large number of macrophages that migrated, while local injection of HUCMSCs and exosomes did inhibit the cranial osteolysis and migration. An exosome inhibitor GW4869 significantly increased the osteolysis area in the mice cranium osteolysis model, and increased the number of migrated macrophages. Immunohistochemical results suggested that the expression of CCL2, CCL3 and CD68 in the cranial in Titanium particles mice increased significantly, but was significantly reduced by HUCMSCs or exosomes. HUCMSC and exosomes down\u2010regulate the expression of CCL3\n                      <jats:italic>in vitro<\/jats:italic>\n                      and\n                      <jats:italic>in vivo<\/jats:italic>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>HUCMSCs and HUCMSC\u2010derived exosomes could suppress the titanium particles\u2010induced osteolysis in mice through inhibiting chemokine (C\u2010C motif) ligand 2, chemokine (C\u2010C motif) ligand 3.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/os.13608","type":"journal-article","created":{"date-parts":[[2023,1,31]],"date-time":"2023-01-31T22:21:24Z","timestamp":1675203684000},"page":"888-898","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["<scp>HUCMSC<\/scp>\n                    \u2010derived Exosomes Suppress the Titanium Particles\u2010induced Osteolysis in Mice through Inhibiting\n                    <scp>CCL2<\/scp>\n                    and\n                    <scp>CCL3<\/scp>"],"prefix":"10.1111","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3728-3537","authenticated-orcid":false,"given":"Shixun","family":"Li","sequence":"first","affiliation":[{"name":"Department of Orthopaedic Surgery Sun Yat\u2010Sen Memorial Hospital, Sun Yat\u2010Sen University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chuangran","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery Sun Yat\u2010Sen Memorial Hospital, Sun Yat\u2010Sen University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sipeng","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery Sun Yat\u2010Sen Memorial Hospital, Sun Yat\u2010Sen University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenkang","family":"Wen","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery Sun Yat\u2010Sen Memorial Hospital, Sun Yat\u2010Sen University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenqiang","family":"Luo","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery Sun Yat\u2010Sen Memorial Hospital, Sun Yat\u2010Sen University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changchuan","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery Sun Yat\u2010Sen Memorial Hospital, Sun Yat\u2010Sen University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyan","family":"Wang","sequence":"additional","affiliation":[{"name":"Guangzhou Saliai Stem Cell Science and Technology Co., LTD  Guangzhou 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