{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T14:40:48Z","timestamp":1784904048459,"version":"3.55.0"},"reference-count":53,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2024,4,24]],"date-time":"2024-04-24T00:00:00Z","timestamp":1713916800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81960403"],"award-info":[{"award-number":["81960403"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82060405"],"award-info":[{"award-number":["82060405"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82360436"],"award-info":[{"award-number":["82360436"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004775","name":"Natural Science Foundation of Gansu Province","doi-asserted-by":"publisher","award":["22JR5RA943"],"award-info":[{"award-number":["22JR5RA943"]}],"id":[{"id":"10.13039\/501100004775","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004775","name":"Natural Science Foundation of Gansu Province","doi-asserted-by":"publisher","award":["22JR5RA956"],"award-info":[{"award-number":["22JR5RA956"]}],"id":[{"id":"10.13039\/501100004775","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004775","name":"Natural Science Foundation of Gansu Province","doi-asserted-by":"publisher","award":["23JRRA1500"],"award-info":[{"award-number":["23JRRA1500"]}],"id":[{"id":"10.13039\/501100004775","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004775","name":"Natural Science Foundation of Gansu Province","doi-asserted-by":"publisher","award":["22JR5RA963"],"award-info":[{"award-number":["22JR5RA963"]}],"id":[{"id":"10.13039\/501100004775","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004775","name":"Natural Science Foundation of Gansu Province","doi-asserted-by":"publisher","award":["22JR11RA117"],"award-info":[{"award-number":["22JR11RA117"]}],"id":[{"id":"10.13039\/501100004775","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Orthopaedic Surgery"],"published-print":{"date-parts":[[2024,6]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>Bone marrow mesenchymal stem cells (BMSCs) show significant potential for osteogenic differentiation. However, the underlying mechanisms of osteogenic capability in osteoporosis\u2010derived BMSCs (OP\u2010BMSCs) remain unclear. This study aims to explore the impact of YTHDF3 (YTH N6\u2010methyladenosine RNA binding protein 3) on the osteogenic traits of OP\u2010BMSCs and identify potential therapeutic targets to boost their bone formation ability.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We examined microarray datasets (GSE35956 and GSE35958) from the Gene Expression Omnibus (GEO) to identify potential m<jats:sup>6<\/jats:sup>A regulators in osteoporosis (OP). Employing differential, protein interaction, and machine learning analyses, we pinpointed critical hub genes linked to OP. We further probed the relationship between these genes and OP using single\u2010cell analysis, immune infiltration assessment, and Mendelian randomization. Our in vivo and in vitro experiments validated the expression and functionality of the key hub gene.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Differential analysis revealed seven key hub genes related to OP, with YTHDF3 as a central player, supported by protein interaction analysis and machine learning methodologies. Subsequent single\u2010cell, immune infiltration, and Mendelian randomization studies consistently validated YTHDF3's significant link to osteoporosis. YTHDF3 levels are significantly reduced in femoral head tissue from postmenopausal osteoporosis (PMOP) patients and femoral bone tissue from PMOP mice. Additionally, silencing YTHDF3 in OP\u2010BMSCs substantially impedes their proliferation and differentiation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>YTHDF3 may be implicated in the pathogenesis of OP by regulating the proliferation and osteogenic differentiation of OP\u2010BMSCs.<\/jats:p><\/jats:sec>","DOI":"10.1111\/os.14064","type":"journal-article","created":{"date-parts":[[2024,4,25]],"date-time":"2024-04-25T02:11:24Z","timestamp":1714011084000},"page":"1418-1433","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Unraveling Key <scp>m<sup>6<\/sup>A<\/scp> Modification Regulators Signatures in Postmenopausal Osteoporosis through Bioinformatics and Experimental Verification"],"prefix":"10.1111","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5844-4657","authenticated-orcid":false,"given":"Zhi\u2010wei","family":"Feng","sequence":"first","affiliation":[{"name":"Department of Orthopaedics Lanzhou University Second Hospital  Lanzhou China"},{"name":"Department of Orthopaedics Nanchong Central Hospital, The Second Clinical Institute of North Sichuan Medical College  Nanchong China"},{"name":"Gansu Province Intelligent Orthopedics Industry Technology Center  Lanzhou China"},{"name":"Gansu Province Orthopaedic Clinical Medicine Research Center  Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"He\u2010fang","family":"Xiao","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics Lanzhou University Second Hospital  Lanzhou China"},{"name":"Gansu Province Intelligent Orthopedics Industry Technology Center  Lanzhou China"},{"name":"Gansu Province Orthopaedic Clinical Medicine Research Center  Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xing\u2010wen","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics Lanzhou University Second Hospital  Lanzhou China"},{"name":"Gansu Province Intelligent Orthopedics Industry Technology Center  Lanzhou China"},{"name":"Gansu Province Orthopaedic Clinical Medicine Research Center  Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong\u2010kang","family":"Niu","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics Lanzhou University Second Hospital  Lanzhou China"},{"name":"Gansu Province Intelligent Orthopedics Industry Technology Center  Lanzhou China"},{"name":"Gansu Province Orthopaedic Clinical Medicine Research Center  Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Da\u2010cheng","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics Lanzhou University Second Hospital  Lanzhou China"},{"name":"Gansu Province Intelligent Orthopedics Industry Technology Center  Lanzhou China"},{"name":"Gansu Province Orthopaedic Clinical Medicine Research Center  Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cong","family":"Tian","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics Lanzhou University Second Hospital  Lanzhou China"},{"name":"Gansu Province Intelligent Orthopedics Industry Technology Center  Lanzhou China"},{"name":"Gansu Province Orthopaedic Clinical Medicine Research Center  Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7849-2672","authenticated-orcid":false,"given":"Sheng\u2010hong","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics Lanzhou University Second Hospital  Lanzhou China"},{"name":"Gansu Province Intelligent Orthopedics Industry Technology Center  Lanzhou China"},{"name":"Gansu Province Orthopaedic Clinical Medicine Research Center  Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo","family":"Peng","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics Lanzhou University Second Hospital  Lanzhou China"},{"name":"Gansu Province Intelligent Orthopedics Industry Technology Center  Lanzhou China"},{"name":"Gansu Province Orthopaedic Clinical Medicine Research Center  Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fei","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics Lanzhou University Second Hospital  Lanzhou China"},{"name":"Department of Orthopaedics Nanchong Central Hospital, The Second Clinical Institute of North Sichuan Medical College  Nanchong China"},{"name":"Gansu Province Intelligent Orthopedics Industry Technology Center  Lanzhou China"},{"name":"Gansu Province Orthopaedic Clinical Medicine Research Center  Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bin","family":"Geng","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics Lanzhou University Second Hospital  Lanzhou China"},{"name":"Gansu Province Intelligent Orthopedics Industry Technology Center  Lanzhou China"},{"name":"Gansu Province Orthopaedic Clinical Medicine Research Center  Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming\u2010gang","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics Nanchong Central Hospital, The Second Clinical Institute of North Sichuan Medical College  Nanchong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao\u2010yun","family":"Sheng","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics Lanzhou University Second Hospital  Lanzhou China"},{"name":"Gansu Province Intelligent Orthopedics Industry Technology Center  Lanzhou China"},{"name":"Gansu Province Orthopaedic Clinical Medicine Research Center  Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ya\u2010yi","family":"Xia","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics Lanzhou University Second Hospital  Lanzhou China"},{"name":"Gansu Province Intelligent Orthopedics Industry Technology Center  Lanzhou China"},{"name":"Gansu Province Orthopaedic Clinical Medicine Research Center  Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,4,24]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-76681-2_1"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00391-019-01571-x"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2019.104334"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMcp1513724"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40273-014-0231-1"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1155\/2019\/1730978"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.5582\/bst.2018.01046"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.26233"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio.1432"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.262766.115"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature12730"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature14234"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkx141"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2016.01.012"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/cr.2014.151"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2012.10.015"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2013.10.026"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2015.05.014"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/cr.2017.10"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms12626"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2017.12.006"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb2902"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2014.09.019"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2017.09.003"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-06898-4"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tig.2012.11.003"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gpb.2018.04.002"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/acel.13298"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.18632\/aging.203377"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00774-021-01297-0"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/1873-3468.14145"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21010199"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1905489116"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20030551"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-021-04312-4"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1111\/cpr.13178"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellsig.2024.111038"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bts034"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.285.6.785"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41588-021-00913-z"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0148804"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_2_11_44_1","doi-asserted-by":"publisher","DOI":"10.3892\/etm.2021.10358"},{"key":"e_1_2_11_45_1","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2019.00911"},{"key":"e_1_2_11_46_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M112.377945"},{"key":"e_1_2_11_47_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0012290"},{"key":"e_1_2_11_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.humimm.2013.04.013"},{"key":"e_1_2_11_49_1","doi-asserted-by":"publisher","DOI":"10.1186\/ar3870"},{"key":"e_1_2_11_50_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.1769"},{"key":"e_1_2_11_51_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41556-019-0318-1"},{"key":"e_1_2_11_52_1","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl-2020-322566"},{"key":"e_1_2_11_53_1","doi-asserted-by":"publisher","DOI":"10.1038\/cr.2017.117"},{"key":"e_1_2_11_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2020.04.009"}],"container-title":["Orthopaedic Surgery"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/os.14064","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,3]],"date-time":"2024-06-03T01:55:59Z","timestamp":1717379759000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/os.14064"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,4,24]]},"references-count":53,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2024,6]]}},"alternative-id":["10.1111\/os.14064"],"URL":"https:\/\/doi.org\/10.1111\/os.14064","archive":["Portico"],"relation":{},"ISSN":["1757-7853","1757-7861"],"issn-type":[{"value":"1757-7853","type":"print"},{"value":"1757-7861","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,4,24]]},"assertion":[{"value":"2024-01-03","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-03-26","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-04-24","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}