{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T13:14:57Z","timestamp":1761743697603},"reference-count":30,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2012,10,29]],"date-time":"2012-10-29T00:00:00Z","timestamp":1351468800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pediatric Allergy Immunology"],"published-print":{"date-parts":[[2012,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Several cases of pine nut allergies and anaphylaxis have been reported in the literature, but only few pine nut allergens have been characterized. The aim of this research is to identify through immunoelectrophoretic techniques the major pine nut allergens in a group of children monosensitized to pine nuts.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We studied five children with pine nut allergies and no other sensitization to food except to pine nuts, confirmed by <jats:italic>in vivo<\/jats:italic> (prick test, prick\u2010to\u2010prick) and <jats:italic>in vitro<\/jats:italic> tests (specific <jats:styled-content style=\"fixed-case\">I<\/jats:styled-content>g<jats:styled-content style=\"fixed-case\">E<\/jats:styled-content> determinations [<jats:styled-content style=\"fixed-case\">CAP<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">FEIA<\/jats:styled-content>]). The protein profile of pine nuts was analyzed by Sodium Dodecyl sulfate Polyacrylamide Gel Electrophoresis (<jats:styled-content style=\"fixed-case\">SDS<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PAGE<\/jats:styled-content>). Immunoblotting was performed after incubation of membranes with the sera from the children included in the present study.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Immunoblotting (<jats:styled-content style=\"fixed-case\">SDS<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PAGE<\/jats:styled-content>) demonstrated five similar bands between 6 and 47\u00a0kDa in all the subjects studied.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>These bands should be considered the potential allergens for pine nut allergic children.<\/jats:p><\/jats:sec>","DOI":"10.1111\/pai.12012","type":"journal-article","created":{"date-parts":[[2012,10,29]],"date-time":"2012-10-29T14:45:05Z","timestamp":1351521905000},"page":"761-764","source":"Crossref","is-referenced-by-count":6,"title":["Children monosensitized to pine nuts have similar patterns of sensitization"],"prefix":"10.1111","volume":"23","author":[{"given":"Elio","family":"Novembre","sequence":"first","affiliation":[{"name":"Department of Pediatric Allergy Unit A. 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