{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T14:26:16Z","timestamp":1789655176113,"version":"4.0.1"},"reference-count":30,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2013,4,3]],"date-time":"2013-04-03T00:00:00Z","timestamp":1364947200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pediatric Allergy Immunology"],"published-print":{"date-parts":[[2013,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Case reports of allergy to lupin, due to primary sensitization or cross\u2010reactions with other legumes, are increasing as a consequence of the augmented use of lupin flour in bakery, pasta formulations and other food items. The main allergens that have been associated with the sensitization to lupin are \u03b1\u2010 and \u03b2\u2010conglutins and, to a lesser extent, \u03b3\u2010 and \u03b4\u2010conglutin, but no conclusive data are available so far. The aim of this study was to characterize the sensitization pattern to lupin in a group of 12 Italian children allergic to peanut and identify the specific lupin proteins involved in the cross\u2010reactivity with peanut.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The immunochemical cross\u2010reactivity among peanut and lupin was evaluated by both <jats:italic>in\u00a0vitro<\/jats:italic> immunoblotting and <jats:italic>in\u00a0vivo<\/jats:italic> fresh food skin prick test (<jats:styled-content style=\"fixed-case\">FFSPT<\/jats:styled-content>).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The results showed that \u03b2\u2010conglutin was recognized by cutaneous <jats:styled-content style=\"fixed-case\">I<\/jats:styled-content>g<jats:styled-content style=\"fixed-case\">E<\/jats:styled-content>s from 7\/12 peanut\u2010allergic children in <jats:styled-content style=\"fixed-case\">FFSPT<\/jats:styled-content> and serum <jats:styled-content style=\"fixed-case\">I<\/jats:styled-content>g<jats:styled-content style=\"fixed-case\">E<\/jats:styled-content>s from 5\/12 in immunoblotting, while 4\/12 and 8\/12 patients tested positive to \u03b3\u2010conglutin in <jats:styled-content style=\"fixed-case\">FFSPT<\/jats:styled-content> and immunoblotting, respectively. No significant immunoreactive responses were observed to \u03b1\u2010 and \u03b4\u2010conglutins under non\u2010reducing conditions, but they were bound in <jats:styled-content style=\"fixed-case\">FFSPT<\/jats:styled-content> by the sera of 5\/12 and 3\/12 patients, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>In this group of allergic children, \u03b2\u2010conglutin has been identified as the major lupin allergen involved both <jats:italic>in\u00a0vitro<\/jats:italic> and <jats:italic>in\u00a0vivo<\/jats:italic> cross\u2010reactivity with peanut proteins. The role of \u03b3\u2010conglutin in the cross\u2010reactivity between lupin and peanut proteins was also relevant and clear, despite the observed unspecificity of the immunoblotting responses.<\/jats:p><\/jats:sec>","DOI":"10.1111\/pai.12054","type":"journal-article","created":{"date-parts":[[2013,4,3]],"date-time":"2013-04-03T06:16:44Z","timestamp":1364969804000},"page":"270-275","source":"Crossref","is-referenced-by-count":26,"title":["Characterization of the sensitization profile to lupin in peanut\u2010allergic children and assessment of cross\u2010reactivity risk"],"prefix":"10.1111","volume":"24","author":[{"given":"Cinzia","family":"Ballabio","sequence":"first","affiliation":[{"name":"Dipartimento di Scienze Farmacologiche e Biomolecolari Universit\u00e0 degli Studi di Milano Milan Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena","family":"Pe\u00f1as","sequence":"additional","affiliation":[{"name":"Dipartimento di Scienze Farmacologiche e 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