{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T06:24:10Z","timestamp":1790749450259,"version":"4.1.0"},"reference-count":30,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2014,11,21]],"date-time":"2014-11-21T00:00:00Z","timestamp":1416528000000},"content-version":"vor","delay-in-days":20,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000097","name":"National Center for Research Resources","doi-asserted-by":"publisher","award":["UL1TR000067"],"award-info":[{"award-number":["UL1TR000067"]}],"id":[{"id":"10.13039\/100000097","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100001419","name":"Food Allergy Initiative","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100001419","id-type":"DOI","asserted-by":"crossref"}]},{"name":"The Louis and Rachel Rudin Foundation Inc"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pediatric Allergy Immunology"],"published-print":{"date-parts":[[2014,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Food protein\u2010induced enterocolitis syndrome (<jats:styled-content style=\"fixed-case\">FPIES<\/jats:styled-content>) is a gastrointestinal hypersensitivity disorder with a poorly understood pathophysiology and no biomarkers to aid in diagnosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>To investigate humoral and cellular responses to casein in children with milk\u2010<jats:styled-content style=\"fixed-case\">FPIES<\/jats:styled-content>, including the role of casein\u2010specific (cs) IgA and T\u2010cell mediated <jats:styled-content style=\"fixed-case\">TGF<\/jats:styled-content>\u2010\u03b2 responses.<\/jats:p><\/jats:sec><jats:sec><jats:title>Patients and methods<\/jats:title><jats:p>Thirty\u2010one children previously diagnosed with milk\u2010<jats:styled-content style=\"fixed-case\">FPIES<\/jats:styled-content> were challenged with milk. Twelve age\u2010matched children with <jats:styled-content style=\"fixed-case\">FPIES<\/jats:styled-content> to other foods and 6 milk\u2010tolerant children without a history of <jats:styled-content style=\"fixed-case\">FPIES<\/jats:styled-content> were used as controls. Casein\u2010specific IgE, IgG, IgG4, and IgA were measured in serum and <jats:styled-content style=\"fixed-case\">TGF<\/jats:styled-content>\u2010\u03b2 levels in supernatants of casein\u2010stimulated <jats:styled-content style=\"fixed-case\">PBMC<\/jats:styled-content>s.<\/jats:p><\/jats:sec><jats:sec><jats:title>Result<\/jats:title><jats:p>Twenty\u2010six children with milk\u2010<jats:styled-content style=\"fixed-case\">FPIES<\/jats:styled-content> reacted (active milk\u2010<jats:styled-content style=\"fixed-case\">FPIES<\/jats:styled-content>) and five tolerated milk (milk\u2010<jats:styled-content style=\"fixed-case\">FPIES<\/jats:styled-content> resolved) during food challenge. All of them had significantly lower levels of csIgG, csIgG4, and csIgA than control children (p\u2010value&lt;0.001). There were no <jats:styled-content style=\"fixed-case\">TGF<\/jats:styled-content>\u2010\u03b2 responses in supernatants of active milk\u2010<jats:styled-content style=\"fixed-case\">FPIES<\/jats:styled-content> children.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Children with milk\u2010<jats:styled-content style=\"fixed-case\">FPIES<\/jats:styled-content> have low levels of csIgG, csIgG4, and csIgA. In particular, children with active <jats:styled-content style=\"fixed-case\">FPIES<\/jats:styled-content> to cow's milk have deficient T\u2010cell mediated <jats:styled-content style=\"fixed-case\">TGF<\/jats:styled-content>\u2010\u03b2 responses to casein, rendering <jats:styled-content style=\"fixed-case\">TGF<\/jats:styled-content>\u2010\u03b2 a promising biomarker in identifying children who are likely to experience <jats:styled-content style=\"fixed-case\">FPIES<\/jats:styled-content> reactions to this allergen. Prospective studies are needed to validate these findings, elucidate their role in <jats:styled-content style=\"fixed-case\">FPIES<\/jats:styled-content> pathophysiology, and establish the diagnostic utility of <jats:styled-content style=\"fixed-case\">TGF<\/jats:styled-content>\u2010\u03b2 in milk\u2010induced <jats:styled-content style=\"fixed-case\">FPIES<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/pai.12288","type":"journal-article","created":{"date-parts":[[2014,10,6]],"date-time":"2014-10-06T06:57:57Z","timestamp":1412578677000},"page":"651-656","source":"Crossref","is-referenced-by-count":56,"title":["The role of casein\u2010specific IgA and <scp>TGF<\/scp>\u2010\u03b2 in children with food protein\u2010induced enterocolitis syndrome to milk"],"prefix":"10.1111","volume":"25","author":[{"given":"George N.","family":"Konstantinou","sequence":"first","affiliation":[{"name":"Division of Allergy &amp; Immunology and The Jaffe Food Allergy Research Institute Icahn School of Medicine at Mount Sinai New York NY USA"},{"name":"Department of Allergy and Clinical Immunology 424 General Military Training Hospital Thessaloniki Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ramon","family":"Bencharitiwong","sequence":"additional","affiliation":[{"name":"Division of Allergy &amp; Immunology and The Jaffe Food Allergy Research Institute Icahn School of Medicine at Mount Sinai New York NY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander","family":"Grishin","sequence":"additional","affiliation":[{"name":"Division of Allergy &amp; Immunology and The Jaffe Food Allergy Research Institute Icahn School of Medicine at Mount Sinai New York NY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean\u2010Christoph","family":"Caubet","sequence":"additional","affiliation":[{"name":"Division of Allergy &amp; Immunology and The Jaffe Food Allergy Research Institute Icahn School of Medicine at Mount Sinai New York NY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luda","family":"Bardina","sequence":"additional","affiliation":[{"name":"Division of Allergy &amp; Immunology and The Jaffe Food Allergy Research Institute Icahn School of Medicine at Mount Sinai New York NY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Scott H.","family":"Sicherer","sequence":"additional","affiliation":[{"name":"Division of Allergy &amp; Immunology and The Jaffe Food Allergy Research Institute Icahn School of Medicine at Mount Sinai New York NY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hugh A.","family":"Sampson","sequence":"additional","affiliation":[{"name":"Division of Allergy &amp; Immunology and The Jaffe Food Allergy Research Institute Icahn School of Medicine at Mount Sinai New York NY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Nowak\u2010W\u0119grzyn","sequence":"additional","affiliation":[{"name":"Division of Allergy &amp; Immunology and The Jaffe Food Allergy Research Institute Icahn School of Medicine at Mount Sinai New York NY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,11,21]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2010.10.008"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/MOP.0b013e3283599ca1"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1542\/peds.2008-2029"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1586\/eci.11.13"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2009.03.042"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2010.10.017"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1097\/00005176-200001001-00009"},{"key":"e_1_2_8_9_1","doi-asserted-by":"crossref","first-page":"3773","DOI":"10.4049\/jimmunol.145.11.3773","article-title":"Transforming growth factor beta 1 increases IgA isotype switching at the clonal level","volume":"145","author":"Kim PH","year":"1990","journal-title":"J Immunol"},{"key":"e_1_2_8_10_1","doi-asserted-by":"crossref","first-page":"3411","DOI":"10.4049\/jimmunol.144.9.3411","article-title":"Transforming growth factor\u2010beta 1 is a costimulator for IgA production","volume":"144","author":"Kim PH","year":"1990","journal-title":"J Immunol"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/9781444300062.ch16"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/pai.12143"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2121-7-15"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2005.00705.x"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1203\/00006450-198408000-00016"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2008.01705.x"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2008.09.007"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2011.04.036"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2008.05.043"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.172.5.3252"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1440-1711.1999.00801.x"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2008.01.072"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2222.2003.01699.x"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2011.01.034"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm.158.2.9801014"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.00229"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(00)00044-3"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/cea.12014"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1067\/mai.2002.120562"},{"key":"e_1_2_8_30_1","doi-asserted-by":"crossref","first-page":"5730","DOI":"10.4049\/jimmunol.153.12.5730","article-title":"Regulation of intestinal epithelial barrier function by TGF\u2010beta 1. Evidence for its role in abrogating the effect of a T cell cytokine","volume":"153","author":"Planchon SM","year":"1994","journal-title":"J Immunol"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/ibd.21211"}],"container-title":["Pediatric Allergy and Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fpai.12288","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/pai.12288","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,23]],"date-time":"2023-09-23T17:49:19Z","timestamp":1695491359000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/pai.12288"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,11]]},"references-count":30,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2014,11]]}},"alternative-id":["10.1111\/pai.12288"],"URL":"https:\/\/doi.org\/10.1111\/pai.12288","archive":["Portico"],"relation":{},"ISSN":["0905-6157","1399-3038"],"issn-type":[{"value":"0905-6157","type":"print"},{"value":"1399-3038","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,11]]}}}