{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T09:28:40Z","timestamp":1790242120787,"version":"4.1.0"},"reference-count":23,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2015,9,22]],"date-time":"2015-09-22T00:00:00Z","timestamp":1442880000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pediatric Allergy Immunology"],"published-print":{"date-parts":[[2015,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>A specific allergen sensitization can be demonstrated in approximately half of the vernal keratoconjunctivitis (<jats:styled-content style=\"fixed-case\">VKC<\/jats:styled-content>) patients by conventional allergic tests. The measurement of specific IgE in tears using a multiplex allergen microarray may offer advantages to identify local sensitization to a specific allergen.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>In spring\u2013summer 2011, serum and tears samples were collected from 10 active <jats:styled-content style=\"fixed-case\">VKC<\/jats:styled-content> patients (three females, seven males) and 10 age\u2010matched normal subjects. Skin prick test, symptoms score and full ophthalmological examination were performed. Specific serum and tear IgE were assayed using Immuno<jats:styled-content style=\"fixed-case\">CAP ISAC<\/jats:styled-content>, a microarray containing 103 components derived from 47 allergens.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Normal subjects resulted negative for the presence of specific IgE both in serum and in tears. Of the 10 <jats:styled-content style=\"fixed-case\">VKC<\/jats:styled-content> patients, six resulted positive to specific IgE in serum and\/or tears. In three of these six patients, specific IgE was found positive only in tears. Cross\u2010reactivity between specific markers was found in three patients. Grass, tree, mites, animal but also food allergen\u2010specific IgE were found in tears. Conjunctival provocation test performed out of season confirmed the specific local conjunctival reactivity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Multiple specific IgE measurements with single protein allergens using a microarray technique in tear samples are a useful, simple and non\u2010invasive diagnostic tool. Immuno<jats:styled-content style=\"fixed-case\">CAP ISAC<\/jats:styled-content> detects allergen sensitization at component level and adds important information by defining both cross\u2010 and co\u2010sensitization to a large variety of allergen molecules. The presence of specific IgE only in tears of <jats:styled-content style=\"fixed-case\">VKC<\/jats:styled-content> patients reinforces the concept of possible local sensitization.<\/jats:p><\/jats:sec>","DOI":"10.1111\/pai.12450","type":"journal-article","created":{"date-parts":[[2015,8,1]],"date-time":"2015-08-01T07:36:53Z","timestamp":1438414613000},"page":"641-645","source":"Crossref","is-referenced-by-count":35,"title":["Microarray\u2010based IgE detection in tears of patients with vernal keratoconjunctivitis"],"prefix":"10.1111","volume":"26","author":[{"given":"Andrea","family":"Leonardi","sequence":"first","affiliation":[{"name":"Ophthalmology Unit Department of Neuroscience University of Padova Padova Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Franco","family":"Borghesan","sequence":"additional","affiliation":[{"name":"Private Practice Allergy and Clinical Immunology Padova Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diego","family":"Faggian","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences \u2013 DSB University of Padova Padova taly"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mario","family":"Plebani","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences \u2013 DSB University of Padova Padova taly"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,9,22]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/pai.12197"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1136\/bjo.2007.117812"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0420.2005.00622.x"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0161-6420(00)00092-0"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/all.12009"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1097\/ACI.0b013e3282ef8674"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1159\/000233640"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/1939-4551-6-17"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ophtha.2009.11.043"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1186\/2045-7022-3-3"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1001\/archopht.1990.01070030090035"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/eye.1993.93"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/eye.1998.68"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.2332\/allergolint.09-OA-0101"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.3109\/02713683.2012.689069"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/eye.1990.109"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.1996.tb00059.x"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1097\/ICO.0b013e3181f23932"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/eye.2001.65"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/eye.2008.372"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2012.02.032"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2013.10.034"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2013.07.019"}],"container-title":["Pediatric Allergy and Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fpai.12450","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/pai.12450","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,2]],"date-time":"2023-10-02T09:37:25Z","timestamp":1696239445000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/pai.12450"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,9,22]]},"references-count":23,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2015,11]]}},"alternative-id":["10.1111\/pai.12450"],"URL":"https:\/\/doi.org\/10.1111\/pai.12450","archive":["Portico"],"relation":{},"ISSN":["0905-6157","1399-3038"],"issn-type":[{"value":"0905-6157","type":"print"},{"value":"1399-3038","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,9,22]]}}}