{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T10:24:51Z","timestamp":1773051891341,"version":"3.50.1"},"reference-count":34,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2017,1,24]],"date-time":"2017-01-24T00:00:00Z","timestamp":1485216000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003196","name":"Ministero della Salute","doi-asserted-by":"publisher","award":["PE\u20102011\u201002348447"],"award-info":[{"award-number":["PE\u20102011\u201002348447"]}],"id":[{"id":"10.13039\/501100003196","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006628","name":"Mead Johnson Nutrition","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100006628","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pediatric Allergy Immunology"],"published-print":{"date-parts":[[2017,5]]},"abstract":"Abstract<\/jats:title>Background<\/jats:title>Extensively hydrolyzed casein formula (EHCF<\/jats:styled-content>) has been proposed for the prevention and is commonly used for the treatment of cow's milk allergy (CMA<\/jats:styled-content>). The addition of the probiotic Lactobacillus rhamnosus <\/jats:italic>GG<\/jats:styled-content> (LGG<\/jats:styled-content>) to EHCF<\/jats:styled-content> may induce faster acquisition of tolerance to cow's milk. The mechanisms underlying this effect are largely unexplored. We investigated the effects of EHCF<\/jats:styled-content> alone or in combination with LGG<\/jats:styled-content> on \u03b2\u2010lactoglobulin (BLG<\/jats:styled-content>) sensitization in mice.<\/jats:p><\/jats:sec>Methods<\/jats:title>Three\u2010week\u2010old C3H\/HeOuJ mice were sensitized by oral administration of BLG<\/jats:styled-content> using cholera toxin as adjuvant at weekly intervals for 5 weeks (sensitization period). Two experimental phases were conducted: (i) EHCF<\/jats:styled-content> or EHCF<\/jats:styled-content>+LGG<\/jats:styled-content> given daily, starting 2 weeks before the sensitization period and then given daily for 5 weeks and (ii) EHCF<\/jats:styled-content> or EHCF<\/jats:styled-content>+LGG<\/jats:styled-content> given daily for 4 weeks, starting 1 week after the sensitization period. Diet free of cow's milk protein was used as control. Acute allergic skin response, anaphylactic symptom score, body temperature, intestinal permeability, anti\u2010BLG<\/jats:styled-content> serum IgE, and interleukin (IL<\/jats:styled-content>)\u20104, IL<\/jats:styled-content>\u20105, IL<\/jats:styled-content>\u201010, IL<\/jats:styled-content>\u201013, IFN<\/jats:styled-content>\u2010\u03b3 mRNA<\/jats:styled-content> expression were analyzed. Peptide fractions of EHCF<\/jats:styled-content> were characterized by reversed\u2010phase (RP<\/jats:styled-content>)\u2010HPLC<\/jats:styled-content>, MALDI<\/jats:styled-content>\u2010TOF<\/jats:styled-content> mass spectrometry, and nano\u2010HPLC<\/jats:styled-content>\/ESI<\/jats:styled-content>\u2010MS<\/jats:styled-content>\/MS<\/jats:styled-content>.<\/jats:p><\/jats:sec>Results<\/jats:title>Extensively hydrolyzed casein formula administration before or after BLG<\/jats:styled-content>\u2010induced sensitization significantly reduced acute allergic skin reaction, anaphylactic symptom score, body temperature decrease, intestinal permeability increase, IL<\/jats:styled-content>\u20104, IL<\/jats:styled-content>\u20105, IL<\/jats:styled-content>\u201013, and anti\u2010BLG<\/jats:styled-content> IgE production. EHCF<\/jats:styled-content> increased expression of IFN<\/jats:styled-content>\u2010\u03b3 and IL<\/jats:styled-content>\u201010. Many of these effects were significantly enhanced by LGG<\/jats:styled-content> supplementation. The peptide panels were similar between the two study formulas and contained sequences that could have immunoregulatory activities.<\/jats:p><\/jats:sec>Conclusions<\/jats:title>The data support dietary intervention with EHCF<\/jats:styled-content> for CMA<\/jats:styled-content> prevention and treatment through a favorable immunomodulatory action. The observed effects are significantly enhanced by LGG<\/jats:styled-content> supplementation.<\/jats:p><\/jats:sec>","DOI":"10.1111\/pai.12687","type":"journal-article","created":{"date-parts":[[2016,12,19]],"date-time":"2016-12-19T20:56:40Z","timestamp":1482181000000},"page":"230-237","source":"Crossref","is-referenced-by-count":37,"title":["Extensively hydrolyzed casein formula alone or with L. rhamnosus <\/i>GG<\/scp> reduces \u03b2\u2010lactoglobulin sensitization in mice"],"prefix":"10.1111","volume":"28","author":[{"given":"Rosita","family":"Aitoro","sequence":"first","affiliation":[{"name":"Department of Translational Medical Science University of Naples \u2018Federico II\u2019 Naples Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raffaele","family":"Simeoli","sequence":"additional","affiliation":[{"name":"Department of Pharmacy University of Naples \u2018Federico II\u2019 Naples 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