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Our aim was to investigate the suitability of a single recombinant hybrid molecule, consisting of the four major timothy grass pollen allergens (Phl p 1, Phl p 2, Phl p 5, and Phl p 6) for in vivo diagnosis of genuine grass pollen allergy in children suffering from pollinosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Sixty\u2010four children aged from 6 to 17\u00a0years with a positive skin reaction and\/or specific IgE to grass pollen extract and respiratory symptoms of pollinosis as well as 9 control children with allergy to other allergen sources were studied. SPT was performed with the recombinant hybrid, the four recombinant timothy grass pollen allergens, and grass pollen extract. Specific IgE reactivity to 176 micro\u2010arrayed allergen molecules was determined using ImmunoCAP ISAC technology. IgE reactivity to the hybrid was detected by non\u2010denaturing RAST\u2010based dot blot assay.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Genuine grass pollen sensitization was confirmed in 94% of the children with positive SPT to grass pollen extract by SPT and IgE reactivity to the hybrid. The four hybrid\u2010negative children showed IgE reactivity to cross\u2010reactive allergens such as Phl p 4, Phl p 11, and Phl p 12 and had also sensitizations to pollen allergens from unrelated plants.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The recombinant hybrid molecule represents a useful tool for in vivo diagnosis of genuine grass pollen sensitization.<\/jats:p><\/jats:sec>","DOI":"10.1111\/pai.12991","type":"journal-article","created":{"date-parts":[[2018,10,14]],"date-time":"2018-10-14T09:18:20Z","timestamp":1539508700000},"page":"59-65","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Detection of genuine grass pollen sensitization in children by skin testing with a recombinant grass pollen hybrid"],"prefix":"10.1111","volume":"30","author":[{"given":"Nikolaos","family":"Douladiris","sequence":"first","affiliation":[{"name":"Allergy Department, 2nd Pediatric Clinic University of Athens Athens Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victoria","family":"Garib","sequence":"additional","affiliation":[{"name":"Division of Immunopathology, Department of Pathophysiology and Allergy Research, Center of Pathophysiology, Infectiology and Immunology Medical University of Vienna Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Margit","family":"Focke\u2010Tejkl","sequence":"additional","affiliation":[{"name":"Division of Immunopathology, Department of Pathophysiology and Allergy Research, Center of Pathophysiology, Infectiology and Immunology Medical University of Vienna Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rudolf","family":"Valenta","sequence":"additional","affiliation":[{"name":"Division of Immunopathology, Department of Pathophysiology and Allergy Research, Center of Pathophysiology, Infectiology and Immunology Medical University of Vienna Vienna Austria"},{"name":"NRC Institute of Immunology FMBA of Russia Moscow Russia"},{"name":"Laboratory for Immunopathology, Department of Clinical Immunology and Allergy Sechenov First Moscow State Medical University  Moscow Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4448-3468","authenticated-orcid":false,"given":"Nikolaos G.","family":"Papadopoulos","sequence":"additional","affiliation":[{"name":"Allergy Department, 2nd Pediatric Clinic University of Athens Athens Greece"},{"name":"Division of Infection, Immunity &amp; Respiratory Medicine University of Manchester  Manchester UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6857-3049","authenticated-orcid":false,"given":"Birgit","family":"Linhart","sequence":"additional","affiliation":[{"name":"Division of Immunopathology, Department of Pathophysiology and Allergy Research, Center of Pathophysiology, Infectiology and Immunology 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