{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T22:01:29Z","timestamp":1790632889045,"version":"4.1.0"},"reference-count":24,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2019,3,5]],"date-time":"2019-03-05T00:00:00Z","timestamp":1551744000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Ministry of Higher Education and Scientific Research of Algeria"},{"name":"Human Nutrition division: Financial support ,"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pediatric Allergy Immunology"],"published-print":{"date-parts":[[2019,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Partially hydrolyzed milk formulas have been proposed for primary prevention in at\u2010risk infants, but evidence of their efficiency and elucidation of the underlying mechanisms are still lacking. Thanks to a Th2\u2010biased mouse model mimicking at\u2010risk patients, we aimed to assess the potency of a partially hydrolyzed whey formula (pHWF) to induce oral tolerance thus preventing further cow's milk (CM) allergy.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>BALB\/c mice were gavaged with pHWF, standard milk formula (SF), or vehicle only (PBS+). All mice were then orally sensitized to CM using cholera toxin and further chronically exposed to CM. Humoral (IgE, IgG1, IgG2a) and cellular (Th2\/Th1\/Th17 cytokine secretion; frequency of CD4+GATA3+ and CD4+CD25+Foxp3+ T cells in the spleen) responses against \u03b2\u2010lactoglobulin (BLG) and whole caseins (CAS) were assessed, as well as a marker of elicitation of allergic reaction (mMCP\u20101) released after an oral challenge with CM.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>All markers of sensitization and of allergic reaction were evidenced in the PBS+ mice and were significantly enhanced upon chronic exposure. Gavage with SF totally and durably prevented sensitization and elicitation of the allergic reaction. Conversely, pre\u2010treatment with pHWF only reduced BLG\u2010specific sensitization (IgE, Th2 cytokines), with no significant effect on sensitization to caseins. However, pHWF pre\u2010treatment significantly reduced mMCP\u20101 concentration in plasma after CM challenges. CD4+CD25+Foxp3+ Treg cell frequency could not be correlated with tolerance efficiency.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Partially hydrolyzed whey formula only partially prevents the further development of CM allergy in this Th2\u2010biased model. A hydrolysate from both whey and casein fractions may be more efficient.<\/jats:p><\/jats:sec>","DOI":"10.1111\/pai.13017","type":"journal-article","created":{"date-parts":[[2019,1,24]],"date-time":"2019-01-24T17:00:26Z","timestamp":1548349226000},"page":"370-377","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Evaluation of the efficiency of hydrolyzed whey formula to prevent cow\u2019s milk allergy in the BALB\/c mouse model"],"prefix":"10.1111","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3186-9142","authenticated-orcid":false,"given":"Amina","family":"Chikhi","sequence":"first","affiliation":[{"name":"Laboratoire d\u2019Immuno\u2010Allergie Alimentaire, UMR Service de Pharmacologie et d'Immunoanalyse, CEA, INRA Universit\u00e9 Paris\u2010Saclay Gif\u2010sur\u2010Yvette France"},{"name":"Laboratoire de Physiologie de la Nutrition et de S\u00e9curit\u00e9 Alimentaire Universit\u00e9 Oran 1 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