{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,5]],"date-time":"2026-11-05T09:57:01Z","timestamp":1793872621588,"version":"4.6.1"},"reference-count":30,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2019,12,11]],"date-time":"2019-12-11T00:00:00Z","timestamp":1576022400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004359","name":"Vetenskapsr\u00e5det","doi-asserted-by":"publisher","award":["2016\u201001698"],"award-info":[{"award-number":["2016\u201001698"]}],"id":[{"id":"10.13039\/501100004359","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pediatric Allergy Immunology"],"published-print":{"date-parts":[[2020,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Early colonization with a diverse microbiota seems to play a crucial role for appropriate immune maturation during childhood. Breastmilk microbiota is one important source of microbes for the infant, transferred together with maternal IgA antibodies. We previously observed that allergy development during childhood was associated with aberrant IgA responses to the gut microbiota already at 1\u00a0month of age, when the IgA antibodies are predominantly maternally derived in breastfed infants.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>To determine the microbial composition and IgA\u2010coated bacteria in breastmilk in relation to allergy development in children participating in an intervention trial with pre\u2010 and post\u2010natal <jats:italic>Lactobacillus reuteri<\/jats:italic> supplementation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A combination of flow cytometric cell sorting and 16S rRNA gene sequencing was used to characterize the bacterial recognition patterns by IgA in breastmilk samples collected one month <jats:italic>post\u2010partum<\/jats:italic> from 40 mothers whose children did or did not develop allergic and asthmatic symptoms during the first 7\u00a0years of age.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The milk fed to children developing allergic manifestations had significantly lower bacterial richness, when compared to the milk given to children that remained healthy. Probiotic treatment influenced the breastmilk microbiota composition. However, the proportions of IgA\u2010coated bacteria, the total bacterial load and the patterns of IgA\u2010coating were similar in breastmilk between mothers of healthy children and those developing allergies.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Consumption of breastmilk with a reduced microbial richness in the first month of life may play an important role in allergy development during childhood.<\/jats:p><\/jats:sec>","DOI":"10.1111\/pai.13176","type":"journal-article","created":{"date-parts":[[2019,11,18]],"date-time":"2019-11-18T07:05:10Z","timestamp":1574060710000},"page":"250-257","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":56,"title":["Allergy development is associated with consumption of breastmilk with a reduced microbial richness in the first month of life"],"prefix":"10.1111","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1131-4342","authenticated-orcid":false,"given":"Majda","family":"Dzidic","sequence":"first","affiliation":[{"name":"Department of Biotechnology Institute of Agrochemistry and Food Technology (IATA\u2010CSIC) Unit of Lactic Acid Bacteria and Probiotics Valencia Spain"},{"name":"Department of Health and Genomics Center for Advanced Research in Public Health FISABIO Valencia Spain"},{"name":"Department of Clinical and Experimental Medicine Division of Autoimmunity and Immune Regulation Link\u00f6ping University Link\u00f6ping Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alex","family":"Mira","sequence":"additional","affiliation":[{"name":"Department of Health and Genomics Center for Advanced Research in Public Health FISABIO Valencia Spain"},{"name":"CIBER\u2010ESP Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alejandro","family":"Artacho","sequence":"additional","affiliation":[{"name":"Department of Health and Genomics Center for Advanced Research in Public Health FISABIO Valencia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas R.","family":"Abrahamsson","sequence":"additional","affiliation":[{"name":"Department of Clinical and Experimental Medicine and Department of Pediatrics Link\u00f6ping University Link\u00f6ping Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2117-5366","authenticated-orcid":false,"given":"Maria C.","family":"Jenmalm","sequence":"additional","affiliation":[{"name":"Department of Clinical and Experimental Medicine Division of Autoimmunity and Immune Regulation Link\u00f6ping University Link\u00f6ping Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6204-4864","authenticated-orcid":false,"given":"Maria Carmen","family":"Collado","sequence":"additional","affiliation":[{"name":"Department of Biotechnology Institute of Agrochemistry and Food Technology (IATA\u2010CSIC) Unit of Lactic Acid Bacteria and Probiotics Valencia 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