{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T07:53:36Z","timestamp":1761897216770,"version":"3.40.5"},"reference-count":27,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2020,1,28]],"date-time":"2020-01-28T00:00:00Z","timestamp":1580169600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pediatric Allergy Immunology"],"published-print":{"date-parts":[[2020,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Hydrolyzed cow's milk protein formulas are widely used for infants with a history or risk of cow's milk allergy. Based on the current theory that food allergen sensitization occurs via the skin, we investigated the epicutaneous immunogenicity of partially hydrolyzed whey proteins, which are ingredients in infant formulas.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>BALB\/c mice were exposed epicutaneously to whey protein concentrate (WPC) or partial whey protein hydrolysates (PWH1 or PWH2) on tape\u2010stripped skin. Sensitization was assessed by evaluating serum \u03b2\u2010lactoglobulin (\u03b2\u2010LG)\u2010specific antibodies, basophil activation, and cytokine production from \u03b2\u2010LG\u2010stimulated lymphoid cells. The anaphylaxis reaction was evaluated by measuring the rectal temperature and plasma level of mouse mast cell protease\u20101 after oral \u03b2\u2010LG challenge. Immune cell accumulation in the skin was also analyzed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Substantive sensitization and \u03b2\u2010LG\u2010induced anaphylaxis reaction were observed in WPC\u2010exposed mice, whereas no significant changes were observed in PWH1\u2010 or PWH2\u2010exposed mice. The basophil and eosinophil counts increased in WPC\u2010exposed murine skin, not but in PWH1\u2010 or PWH2\u2010exposed mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The epicutaneous immunogenicity of PWH1 and PWH2 is markedly decreased, which may reduce the risk of allergen sensitization. Further studies are required to investigate the clinical value of these partial\u00a0hydrolysates for high\u2010risk infants.<\/jats:p><\/jats:sec>","DOI":"10.1111\/pai.13211","type":"journal-article","created":{"date-parts":[[2020,1,14]],"date-time":"2020-01-14T07:19:11Z","timestamp":1578986351000},"page":"388-395","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Epicutaneous immunogenicity of partially hydrolyzed whey protein evaluated using tape\u2010stripped mouse model"],"prefix":"10.1111","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3238-2192","authenticated-orcid":false,"given":"Hiroshi","family":"Iwamoto","sequence":"first","affiliation":[{"name":"Wellness &amp; Nutrition Science Institute Morinaga Milk Industry Co., Ltd. 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