{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T18:30:10Z","timestamp":1777573810021,"version":"3.51.4"},"reference-count":25,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2020,6,26]],"date-time":"2020-06-26T00:00:00Z","timestamp":1593129600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pediatric Allergy Immunology"],"published-print":{"date-parts":[[2020,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Grass carp is the most commonly consumed fish species in Hong Kong. The allergenicity of grass carp and its allergen content are yet to be reported. This study characterized the major allergen in grass carp and investigated its allergenicity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Sixty\u2010nine subjects with history of IgE\u2010mediated allergic reaction to grass carp were recruited. The protein content in steamed grass carp extract was resolved by SDS\u2010PAGE, and the major allergen was identified by immunoblotting with serum from subjects allergic to grass carp. The identity of allergen was elucidated by mass spectrometry and amino acid sequence obtained by amplifying the specific gene from cDNA library of grass carp. The cross\u2010reactivity between parvalbumins from grass carp and other phylogenetically close (common carp) or commercially important (cod and salmon) species was investigated by competitive inhibition ELISA.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A major IgE\u2010binding protein was found at approximately 9\u00a0kDa and identified as parvalbumin by immunoblotting and mass spectrometry. Grass carp parvalbumin was more allergenic than common carp, salmon, and cod parvalbumins despite sharing high sequence homology. This newly identified major allergenic parvalbumin isoform from grass carp was registered as <jats:italic>Cten i 1<\/jats:italic> in the World Health Organization and International Union of Immunological Societies allergen database.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Grass carp parvalbumin is identified as the major fish allergen in Hong Kong. The strong allergenicity of <jats:italic>Cten i 1<\/jats:italic> contributes to the high IgE reactivity of grass carp. Grass carp, among other fish species, should be considered when managing fish\u2010allergic patients.<\/jats:p><\/jats:sec>","DOI":"10.1111\/pai.13259","type":"journal-article","created":{"date-parts":[[2020,4,23]],"date-time":"2020-04-23T05:23:40Z","timestamp":1587619420000},"page":"792-804","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Molecular and immunological characterization of grass carp (<i>Ctenopharyngodon idella<\/i>) parvalbumin <i>Cten i 1<\/i>: A major fish allergen in Hong Kong"],"prefix":"10.1111","volume":"31","author":[{"given":"Nicki Yat Hin","family":"Leung","sequence":"first","affiliation":[{"name":"Department of Paediatrics The Chinese University of Hong Kong Prince of Wales Hospital Shatin Hong Kong"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Agnes Sze Yin","family":"Leung","sequence":"additional","affiliation":[{"name":"Department of Paediatrics The Chinese University 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