{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T09:27:27Z","timestamp":1793179647557,"version":"4.5.0"},"reference-count":39,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2021,5,7]],"date-time":"2021-05-07T00:00:00Z","timestamp":1620345600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pediatric Allergy Immunology"],"published-print":{"date-parts":[[2021,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Anaphylaxis is the most severe manifestation of allergic disorders. The poor knowledge of its molecular mechanisms often leads to under\u2010diagnosis. MicroRNAs (miRNA) regulate physiologic and pathologic processes, and they have been postulated as promising diagnostic markers. The main objectives of this study were to characterize the human miRNA profile during anaphylaxis and to assess their capacity as diagnostic markers and determine their participation in the molecular mechanisms of this event.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>The miRNA serum profiles from the acute and baseline phase of 5 oral food\u2010challenged anaphylactic children (&lt;18\u00a0years old) were obtained by next\u2010generation sequencing (NGS). From the panel of statistically significant miRNAs obtained, several candidates were selected and analyzed in 19 anaphylactic children by qPCR. We performed system biology analysis (SBA) on their target genes to identify main functions and canonical pathways. A functional in vitro assay was carried out incubating endothelial cells (ECs) in anaphylactic conditions.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The NGS identified 389 miRNAs among which 41 were significantly different between acute and baseline samples. The high levels of miR\u201021\u20103p (fold change\u00a0=\u00a02.28,\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0=\u00a0.006) and miR\u2010487b\u20103p (fold change\u00a0=\u00a01.04,\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0=\u00a0.039) observed by NGS in acute serum samples were confirmed in a larger group of 19 patients. The SBA revealed molecular pathways related to the inflammation and immune system regulation. miR\u201021\u20103p increased intracellularly and in acute phase serum after EC stimulation.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>These findings provide, for the first time, some insights into the anaphylactic miRNA serum profile in children and point to miR\u201021\u20103p and miR\u2010487b\u20103p as candidate biomarkers. Furthermore, the SBA revealed a possible implication of these molecules in the underlying molecular mechanisms. Moreover, ECs increased miR\u201021\u20103p intracellularly and released it to the environment in response to anaphylaxis.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/pai.13518","type":"journal-article","created":{"date-parts":[[2021,4,20]],"date-time":"2021-04-20T01:26:06Z","timestamp":1618881966000},"page":"1296-1306","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Increased miR\u201021\u20103p and miR\u2010487b\u20103p serum levels during anaphylactic reaction in food allergic children"],"prefix":"10.1111","volume":"32","author":[{"given":"Emilio","family":"Nu\u00f1ez\u2010Borque","sequence":"first","affiliation":[{"name":"Department of Allergy and Immunology IIS\u2010Fundaci\u00f3n Jim\u00e9nez D\u00edaz UAM Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergio","family":"Fernandez\u2010Bravo","sequence":"additional","affiliation":[{"name":"Department of Allergy and Immunology IIS\u2010Fundaci\u00f3n Jim\u00e9nez D\u00edaz UAM Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0783-1988","authenticated-orcid":false,"given":"Pablo","family":"Rodriguez Del Rio","sequence":"additional","affiliation":[{"name":"Allergy Department Foundation for Biomedical Research Ni\u00f1o Jesus University Children\u2019s Hospital Madrid Spain"},{"name":"Instituto de Salud Carlos III ARADyAL Network Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ebrahim Mohammed","family":"Alwashali","sequence":"additional","affiliation":[{"name":"CAI Genomics and Proteomics Proteomic Unit Faculty of Pharmacy Complutense University of Madrid Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Lopez\u2010Dominguez","sequence":"additional","affiliation":[{"name":"Clinical Biostatistics Unit Instituto de Investigaci\u00f3n Puerta de Hierro (IDIPHIM) Puerta de Hierro Majadahonda University Hospital Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Dolores","family":"Gutierrez\u2010Blazquez","sequence":"additional","affiliation":[{"name":"CAI Genomics and Proteomics Proteomic Unit Faculty of Pharmacy Complutense University of Madrid Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5909-8979","authenticated-orcid":false,"given":"Jose Julio","family":"Laguna","sequence":"additional","affiliation":[{"name":"Instituto de Salud Carlos III ARADyAL Network Madrid Spain"},{"name":"Allergy Unit, Allergo\u2010Anaesthesia Unit Faculty of Medicine Hospital Central de la Cruz Roja Alfonso X El Sabio University Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4442-3649","authenticated-orcid":false,"given":"Jaime","family":"Tome\u2010Amat","sequence":"additional","affiliation":[{"name":"Instituto 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