{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T21:43:49Z","timestamp":1784324629988,"version":"3.55.0"},"reference-count":27,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2023,8,21]],"date-time":"2023-08-21T00:00:00Z","timestamp":1692576000000},"content-version":"vor","delay-in-days":20,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pediatric Allergy Immunology"],"published-print":{"date-parts":[[2023,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>The gold standard for diagnosing egg allergy in children is the oral food challenge (OFC). However, OFCs are time\u2010consuming and risky procedures. Our study aimed to evaluate the utility of the basophil activation test (BAT) and component\u2010resolved diagnostic in the diagnostic workup of children with egg allergy.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Overall, 86 children aged 6\u2009months to 17\u2009years, suspected of egg allergy, underwent OFC with boiled egg according to international standardized protocols. BAT and specific immunoglobulin E (sIgE) testing to component egg proteins (Gal d 1\u20104) were also performed.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Of the 22 children who reacted to boiled egg, only one experienced anaphylaxis during the challenge. BAT was performed in samples obtained by 75 of the 86 patients of our cohort. Egg white and yolk protein extracts induced CD63 upregulation in the egg\u2010allergic (EA) children compared with sensitized children that tolerated boiled egg (we registered an overall mean of CD63 expression in the EA population of 44.4% [SD 34.1] for egg white and 34.7% [SD 31.3] for egg yolk vs. 12.5% [SD 19.1] and 10.0% [SD 16.0] in sensitized children). BAT could discriminate between true egg allergy and egg sensitization in our population. As a second\u2010line diagnostic step, the positivity of BAT for egg white or Gal d 1\u2010sIgE resulted in a 40.9% OFC reduction, especially for those with a positive outcome.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The BAT may be implemented in the diagnostic workup of egg allergy in children and, in a stepwise approach, separately or combined with Gal d 1\u2010sIgE, may predict the allergic status and reduce the number of positive OFCs in children with egg allergy at low risk for severe reactions.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/pai.14012","type":"journal-article","created":{"date-parts":[[2023,8,22]],"date-time":"2023-08-22T02:45:59Z","timestamp":1692672359000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["The role of basophil activation test and component\u2010resolved diagnostics in the workup of egg allergy in children at low risk for severe allergic reactions: A real\u2010life study"],"prefix":"10.1111","volume":"34","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1773-6482","authenticated-orcid":false,"given":"Amelia","family":"Licari","sequence":"first","affiliation":[{"name":"Department of Clinical, Surgical, Diagnostic and Pediatric Sciences, Pediatric Unit University of Pavia  Pavia Italy"},{"name":"Pediatric Clinic Fondazione IRCCS Policlinico San Matteo  Pavia Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2750-5810","authenticated-orcid":false,"given":"Enza","family":"D'Auria","sequence":"additional","affiliation":[{"name":"Allergy Unit Vittore Buzzi Children's Hospital, University of Milan  Milan Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mara","family":"De\u00a0Amici","sequence":"additional","affiliation":[{"name":"Immuno\u2010Allergology Laboratory, Clinical Chemistry Unit Fondazione IRCCS Policlinico San Matteo  Pavia Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Riccardo","family":"Castagnoli","sequence":"additional","affiliation":[{"name":"Department of Clinical, Surgical, Diagnostic and Pediatric Sciences, Pediatric Unit University of Pavia  Pavia Italy"},{"name":"Pediatric Clinic Fondazione IRCCS Policlinico San Matteo  Pavia Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucia","family":"Sacchi","sequence":"additional","affiliation":[{"name":"Department of Electrical, Computer and Biomedical Engineering, Laboratory for Biomedical Informatics \"Mario Stefanelli\" University of Pavia  Pavia Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giorgia","family":"Testa","sequence":"additional","affiliation":[{"name":"Pediatric Clinic Fondazione IRCCS Policlinico San Matteo  Pavia Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3662-0159","authenticated-orcid":false,"given":"Gian Luigi","family":"Marseglia","sequence":"additional","affiliation":[{"name":"Department of Clinical, Surgical, Diagnostic and Pediatric Sciences, Pediatric Unit University of Pavia  Pavia Italy"},{"name":"Pediatric Clinic Fondazione IRCCS Policlinico San Matteo  Pavia 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