{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T18:55:37Z","timestamp":1786992937174,"version":"build-2736575974"},"reference-count":37,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2025,12,10]],"date-time":"2025-12-10T00:00:00Z","timestamp":1765324800000},"content-version":"vor","delay-in-days":9,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T00:00:00Z","timestamp":1764547200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pediatric Allergy Immunology"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>The relationship between the severity of peanut allergy and component\u2010specific immunoglobulin E (IgE) remains partially analyzed. We aimed to explore this relationship using a proteomic analysis of pediatric patients with peanut allergy.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Immunoblotting and mass spectrometry were used to identify candidate peanut allergen components, which were confirmed via enzyme\u2010linked immunosorbent assay using sera from pediatric patients with peanut allergy confirmed through a positive oral food challenge test (OFC). The association between each protein\u2010specific IgE level and the severity of peanut allergy was compared. The severity of peanut allergy was quantified as TS\/Pro, which is the total score (TS) of Anaphylaxis Scoring Aichi divided by the cumulative protein dose of peanuts at the OFC (Pro).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      This study comprised 52 patients with peanut allergy. In addition to the known peanut allergen components, we discovered seven allergens in more than five participants. Among the participants, 24 (46.2%) had Annexin Gh1\u2010specific IgE. Ara h 2 (\n                      <jats:italic>r<\/jats:italic>\n                      s\u2009=\u20090.67,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u2009.001) and Ara h 6 (\n                      <jats:italic>r<\/jats:italic>\n                      s\u2009=\u20090.66,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u2009.001) specific IgEs and the sum of the absorbance of all seven candidates (\n                      <jats:italic>r<\/jats:italic>\n                      s\u2009=\u20090.64,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u2009.001) were strongly correlated with TS\/Pro. Ara h 1 (\n                      <jats:italic>r<\/jats:italic>\n                      s\u2009=\u20090.30,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u2009.05), Ara h 3 (rs\u2009=\u20090.37,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u2009.01), Ara h 7 (\n                      <jats:italic>r<\/jats:italic>\n                      s\u2009=\u20090.34,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u2009.05), and the seed biotin\u2010containing protein SBP65 (\n                      <jats:italic>r<\/jats:italic>\n                      s\u2009=\u20090.35,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u2009.05) specific IgEs were correlated with TS\/Pro.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Ara h 2\u2010 and 6\u2010specific IgEs and sensitization diversity were the most significant factors that correlated with peanut allergy severity. We identified SBP65 (Ara h 20) as a potential novel allergen component related to peanut allergy severity.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/pai.70254","type":"journal-article","created":{"date-parts":[[2025,12,10]],"date-time":"2025-12-10T08:45:01Z","timestamp":1765356301000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Exploration of severity\u2010associated peanut allergens via proteomic analysis"],"prefix":"10.1111","volume":"36","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3660-1823","authenticated-orcid":false,"given":"Teruaki","family":"Matsui","sequence":"first","affiliation":[{"name":"Department of Allergy, Allergy and Immunology Center Aichi Children's Health and Medical Center  Obu Japan"},{"name":"Collaborative Department of Advanced Allergy and Immunology Fujita Health University School of Medicine  Nagoya Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5366-6899","authenticated-orcid":false,"given":"Naoshi","family":"Shimojo","sequence":"additional","affiliation":[{"name":"Collaborative Department of Advanced Allergy and Immunology Fujita Health University School of Medicine  Nagoya Japan"},{"name":"General Research and Development Institute Hoyu Co., Ltd.  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