{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T02:45:24Z","timestamp":1788403524951,"version":"build-2803163510"},"reference-count":26,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,4,12]],"date-time":"2026-04-12T00:00:00Z","timestamp":1775952000000},"content-version":"vor","delay-in-days":11,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pediatric Allergy Immunology"],"published-print":{"date-parts":[[2026,4]]},"abstract":"Abstract<\/jats:title>\n \n Background<\/jats:title>\n Thunderstorm\u2010related asthma (TA) is a distinct environmental health emergency marked by abrupt surges in asthma exacerbations following thunderstorm events. Although peripheral eosinophilia has been implicated as a risk factor, the phenotypic and functional characteristics of eosinophils contributing to TA remain insufficiently defined. This study aimed to delineate the phenotypic characteristics of circulating eosinophil subsets in pediatric patients experiencing thunderstorm\u2010related asthma exacerbations.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n Children presenting with asthma exacerbations within 24\u2009h of a documented thunderstorm event were enrolled as the TA group (\n n<\/jats:italic>\n \u2009=\u200916), whereas age\u2010 and sex\u2010matched children with well\u2010controlled asthma served as controls (WA,\n n<\/jats:italic>\n \u2009=\u200926). Circulating eosinophil subsets were characterized by flow cytometry, and type\u20102 cytokines and eosinophil\u2010associated chemokines in serum were quantified by ELISA. In\u00a0vitro, the blood eosinophils were isolated and stimulated with cytokines (IL\u20105, IL\u20103, and GM\u2010CSF) to assess phenotypic changes and mediator secretion.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n TA patients exhibited markedly higher levels of circulating CD62L\n low<\/jats:sup>\n inflammatory eosinophils (iEOS) compared with WA patients, which was closely correlated with clinical indicators of type\u20102 inflammation. Eosinophils from TA patients demonstrated broadly elevated expression of activation and cytokine receptor markers, including CD69, HLA\u2010DR, CCR3, CD123, and CD125. Upon cytokine stimulation, the enriched eosinophils from TA patients exhibited a pronounced expansion of the CD62L\n low<\/jats:sup>\n subset with a reciprocal reduction in CD62L\n bright<\/jats:sup>\n cells, accompanied by elevated production of Eotaxin\u20101, Eotaxin\u20102, Eotaxin\u20103, and IL\u201013 in the culture supernatant.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n \n Thunderstorm\u2010related asthma is associated with a marked expansion of circulating CD62L\n low<\/jats:sup>\n iEOS that display heightened activation and cytokine responsiveness, indicating a primed type\u20102 inflammatory phenotype in susceptible children. Assessment of CD62L\n low<\/jats:sup>\n iEOS may be beneficial for early identification of children at high risk of thunderstorm\u2010triggered exacerbations and help inform proactive preventive strategies.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/pai.70349","type":"journal-article","created":{"date-parts":[[2026,4,13]],"date-time":"2026-04-13T03:53:01Z","timestamp":1776052381000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Expanded peripheral\n \n CD62L\n low<\/sup>\n <\/scp>\n inflammatory eosinophils in\u00a0pediatric thunderstorm\u2010related asthma exacerbations: A single\u2010center analysis"],"prefix":"10.1111","volume":"37","author":[{"given":"Qing","family":"Miao","sequence":"first","affiliation":[{"name":"Department of Allergy, Beijing Children's Hospital Capital Medical University, National Center for Children's Health Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"XiaoLing","family":"Hou","sequence":"additional","affiliation":[{"name":"Department of Allergy, Beijing Children's Hospital Capital Medical University, National Center for Children's Health Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Xiang","sequence":"additional","affiliation":[{"name":"Department of Allergy, Beijing Children's Hospital Capital Medical University, National Center for Children's Health Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"TaiYuan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Meteorological Medicine and Health China Meteorological Administration Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chen","family":"Liu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Meteorological Medicine and Health China Meteorological 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