{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T18:33:44Z","timestamp":1793385224642,"version":"4.5.0"},"reference-count":37,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T00:00:00Z","timestamp":1786665600000},"content-version":"vor","delay-in-days":13,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82570045"],"award-info":[{"award-number":["82570045"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pediatric Allergy Immunology"],"published-print":{"date-parts":[[2026,8]]},"abstract":"Abstract<\/jats:title>\n \n Background<\/jats:title>\n Prenatal exposure to ambient air pollution has been associated with early\u2010life allergic outcomes; however, underlying biological mechanisms remain incompletely understood.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n A total of 101 mother\u2010infant pairs were ultimately included in this prospective birth cohort study. Maternal exposure to ambient air pollutants was assigned to geocoded residential addresses using spatiotemporal models. Allergic symptoms were defined as cumulative caregiver\u2010reported symptoms occurring from birth to 6\u2009months and assessed at the 6\u2010month follow\u2010up. DNA methylation in na\u00efve CD4\n +<\/jats:sup>\n T cells was analyzed in a subset of 30 cord blood samples using targeted bisulfite sequencing. Associations were evaluated using regression models, and statistical mediation analysis was performed under a counterfactual\u2010based approach.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n Higher first\u2010trimester exposure to PM\n 10<\/jats:sub>\n (adjusted odds ratio [AOR], 2.27; 95% confidence interval [CI], 1.09\u20134.98) and NO\n 2<\/jats:sub>\n (AOR, 2.11; 95% CI, 1.02\u20134.53) was associated with an increased likelihood of allergic symptoms within the first 6\u2009months of life. In addition, third\u2010trimester exposure to PM\n 2.5<\/jats:sub>\n components, including sulfate (SO\n 4<\/jats:sub>\n 2\u2212<\/jats:sup>\n ), nitrate (NO\n 3<\/jats:sub>\n \u2212<\/jats:sup>\n ), and ammonium (NH\n 4<\/jats:sub>\n +<\/jats:sup>\n ), was positively associated with these outcomes. DNA methylation at a CpG site within the\n PRKACB<\/jats:italic>\n promoter showed statistical evidence consistent with a potential mediating role in the association between third\u2010trimester sulfate exposure and allergic symptoms. Functional assays supported the regulatory relevance of this locus.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n \n Prenatal ambient air pollution exposure was associated with early\u2010life allergic symptoms, and cord blood DNA methylation at the\n PRKACB<\/jats:italic>\n locus may represent a potential molecular mechanism underlying these associations. These findings are exploratory and require confirmation in larger studies.\n <\/jats:p>\n \n \n \n image<\/jats:alt-text>\n <\/jats:graphic>\n <\/jats:boxed-text>\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/pai.70424","type":"journal-article","created":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T08:36:01Z","timestamp":1786696561000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Cord blood DNA methylation as a potential mediator in the association between prenatal ambient air pollution and early infant allergic symptoms in a Shanghai birth cohort"],"prefix":"10.1111","volume":"37","author":[{"given":"Xiaoqin","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Otolaryngology Shanghai Children's Medical Center, School of Medicine, Shanghai Jiao Tong University Shanghai China"},{"name":"College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics Fujian Medical University 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