{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T22:30:09Z","timestamp":1769639409008,"version":"3.49.0"},"reference-count":44,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2012,11,2]],"date-time":"2012-11-02T00:00:00Z","timestamp":1351814400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Plant Biotechnology Journal"],"published-print":{"date-parts":[[2013,1]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Plants are subject to attack by a wide range of phytopathogens. Current pathogen detection methods and technologies are largely constrained to those occurring post\u2010symptomatically. Recent efforts were made to generate plant sentinels (phytosensors) that can be used for sensing and reporting pathogen contamination in crops. Engineered phytosensors indicating the presence of plant pathogens as early\u2010warning sentinels potentially have tremendous utility as wide\u2010area detectors. We previously showed that synthetic promoters containing pathogen and\/or defence signalling inducible <jats:italic>cis<\/jats:italic>\u2010acting regulatory elements (<jats:styled-content style=\"fixed-case\">RE<\/jats:styled-content>) fused to a fluorescent protein (<jats:styled-content style=\"fixed-case\">FP<\/jats:styled-content>) reporter could detect phytopathogenic bacteria in a transient phytosensing system. Here, we further advanced this phytosensing system by developing stable transgenic tobacco and <jats:italic><jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>rabidopsis<\/jats:italic> plants containing candidate constructs. The inducibility of each synthetic promoter was examined in response to biotic (bacterial pathogens) or chemical (plant signal molecules salicylic acid, ethylene and methyl jasmonate) treatments using stably transgenic plants. The treated plants were visualized using epifluorescence microscopy and quantified using spectrofluorometry for <jats:styled-content style=\"fixed-case\">FP<\/jats:styled-content> synthesis upon induction. Time\u2010course analyses of <jats:styled-content style=\"fixed-case\">FP<\/jats:styled-content> synthesis showed that both transgenic tobacco and <jats:italic><jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>rabidopsis<\/jats:italic> plants were capable to respond in predictable ways to pathogen and chemical treatments. These results provide insights into the potential applications of transgenic plants as phytosensors and the implementation of emerging technologies for monitoring plant disease outbreaks in agricultural fields.<\/jats:p>","DOI":"10.1111\/pbi.12005","type":"journal-article","created":{"date-parts":[[2012,11,2]],"date-time":"2012-11-02T14:02:00Z","timestamp":1351864920000},"page":"43-52","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Bacterial pathogen phytosensing in transgenic tobacco and <i><scp>A<\/scp>rabidopsis<\/i> plants"],"prefix":"10.1111","volume":"11","author":[{"given":"Wusheng","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Plant Sciences The University of Tennessee Knoxville TN USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mitra","family":"Mazarei","sequence":"additional","affiliation":[{"name":"Department of Plant Sciences The University of Tennessee Knoxville TN USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mary R.","family":"Rudis","sequence":"additional","affiliation":[{"name":"Department of Plant Sciences The University of Tennessee Knoxville TN USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael H.","family":"Fethe","sequence":"additional","affiliation":[{"name":"Department of Plant Sciences The University of Tennessee Knoxville TN USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanhui","family":"Peng","sequence":"additional","affiliation":[{"name":"Department of Plant Sciences The University of Tennessee Knoxville TN USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Reginald J.","family":"Millwood","sequence":"additional","affiliation":[{"name":"Department of Plant Sciences The University of Tennessee Knoxville TN USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gisele","family":"Schoene","sequence":"additional","affiliation":[{"name":"Department of Plant Sciences The University of Tennessee Knoxville TN USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jason N.","family":"Burris","sequence":"additional","affiliation":[{"name":"Department of Plant Sciences The University of Tennessee Knoxville TN USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"Jr","given":"C. 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