{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T20:53:47Z","timestamp":1771016027483,"version":"3.50.1"},"reference-count":53,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2012,10,16]],"date-time":"2012-10-16T00:00:00Z","timestamp":1350345600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Plant Biotechnology Journal"],"published-print":{"date-parts":[[2013,1]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Transgenic rice accumulating the modified major <jats:styled-content style=\"fixed-case\">J<\/jats:styled-content>apanese cedar pollen allergens, <jats:italic><jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>ryptomeria japonica 1<\/jats:italic> (<jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>ry j 1) and <jats:italic><jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>ryptomeria japonica 2<\/jats:italic> (<jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>ry j 2), which were deconstructed by fragmentation and shuffling, respectively, in the edible part of the seed was generated by transformation of a good\u2010tasting rice variety, \u2018<jats:styled-content style=\"fixed-case\">K<\/jats:styled-content>oshihikari\u2019. These modified cedar pollen antigens were deposited in <jats:styled-content style=\"fixed-case\">ER<\/jats:styled-content>\u2010derived protein bodies (<jats:styled-content style=\"fixed-case\">PB<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">I<\/jats:styled-content>), which are suitable for delivery to the mucosal immune system in gut\u2010associated lymphoid tissue when orally administered because antigens bioencapsulated in <jats:styled-content style=\"fixed-case\">PB<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">I<\/jats:styled-content> are resistant against hydrolysis by intestinal enzymes and harsh environments. Mice fed transgenic seeds daily for three weeks and then challenged with crude cedar pollen allergen showed marked suppression of allergen\u2010specific <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>4<jats:sup>+<\/jats:sup> <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>\u2010cell proliferation, <jats:styled-content style=\"fixed-case\">I<\/jats:styled-content>g<jats:styled-content style=\"fixed-case\">E<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">I<\/jats:styled-content>g<jats:styled-content style=\"fixed-case\">G<\/jats:styled-content> levels compared with mice fed nontransgenic rice seeds. As clinical symptoms of pollinosis, sneezing frequency and infiltration of inflammatory cells such as eosinophils and neutrophils were also significantly reduced in the nasal tissue. These results imply that oral administration of transgenic rice seeds containing the structurally disrupted <jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>ry j 1 and <jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>ry j 2 antigens, serving as universal antigens, is a promising approach for specific immunoprophylaxis against <jats:styled-content style=\"fixed-case\">J<\/jats:styled-content>apanese cedar pollinosis.<\/jats:p>","DOI":"10.1111\/pbi.12007","type":"journal-article","created":{"date-parts":[[2012,10,16]],"date-time":"2012-10-16T09:56:22Z","timestamp":1350381382000},"page":"66-76","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":63,"title":["Oral immunotherapy with transgenic rice seed containing destructed <scp>J<\/scp>apanese cedar pollen allergens, <scp>C<\/scp>ry j 1 and <scp>C<\/scp>ry j 2, against <scp>J<\/scp>apanese cedar pollinosis"],"prefix":"10.1111","volume":"11","author":[{"given":"Yuhya","family":"Wakasa","sequence":"first","affiliation":[{"name":"Functional Crop Research and Development Unit National Institute of Agrobiological Sciences Tsukuba Ibaraki Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hidenori","family":"Takagi","sequence":"additional","affiliation":[{"name":"Functional Crop Research and Development Unit National Institute of Agrobiological Sciences Tsukuba Ibaraki Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sakiko","family":"Hirose","sequence":"additional","affiliation":[{"name":"Rice Biotechnology Research Team National Institute of Crop Sciences Tsukuba Ibaraki Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lijun","family":"Yang","sequence":"additional","affiliation":[{"name":"Functional Crop Research and Development Unit National Institute of Agrobiological Sciences Tsukuba Ibaraki Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mayumi","family":"Saeki","sequence":"additional","affiliation":[{"name":"Allergy and Immunology Project Tokyo Metropolitan Institute of Medical Science Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomoe","family":"Nishimura","sequence":"additional","affiliation":[{"name":"Allergy and Immunology Project Tokyo Metropolitan Institute of Medical Science Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Osamu","family":"Kaminuma","sequence":"additional","affiliation":[{"name":"Allergy and Immunology Project Tokyo Metropolitan Institute of Medical Science Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takachika","family":"Hiroi","sequence":"additional","affiliation":[{"name":"Allergy and Immunology Project Tokyo Metropolitan Institute of Medical Science Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fumio","family":"Takaiwa","sequence":"additional","affiliation":[{"name":"Functional Crop Research and Development Unit National Institute of Agrobiological Sciences Tsukuba Ibaraki Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,10,16]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2007.01.022"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1586\/14760584.4.6.881"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2007.06.013"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2006.01237.x"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2008.02.037"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1067\/mai.2003.129"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2011.01.047"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.0105-2896.2005.00280.x"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.2009.03443.x"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1067\/mai.2003.84"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1159\/000316349"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2004.00584.x"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.1995.tb00027.x"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0091-6749(98)70334-3"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s001220050397"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.175.6.3614"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2006.01175.x"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1159\/000312129"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2006.01309.x"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri1934"},{"key":"e_1_2_5_22_1","first-page":"645","article-title":"Molecular design of allergy vaccines","volume":"17","author":"Linhart B.","year":"2005","journal-title":"Curr. Opin. Immumnol."},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri1370"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2005.01.003"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2004.03.017"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1586\/14760584.5.1.103"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.2008.03042.x"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.2332\/allergolint.08-RAI-0074"},{"key":"e_1_2_5_29_1","doi-asserted-by":"publisher","DOI":"10.2332\/allergolint.08-RAI-0072"},{"key":"e_1_2_5_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1467-7652.2010.00502.x"},{"key":"e_1_2_5_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.2009.03242.x"},{"key":"e_1_2_5_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2008.03.013"},{"key":"e_1_2_5_33_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.1990.tb00501.x"},{"key":"e_1_2_5_34_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2567.1997.00255.x"},{"key":"e_1_2_5_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1467-7652.2011.00613.x"},{"key":"e_1_2_5_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiosc.2011.10.005"},{"key":"e_1_2_5_37_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1467-7652.2003.00038.x"},{"key":"e_1_2_5_38_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0503428102"},{"key":"e_1_2_5_39_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1467-7652.2005.00143.x"},{"key":"e_1_2_5_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.peptides.2010.04.032"},{"key":"e_1_2_5_41_1","first-page":"041","article-title":"Transgenic rice seed as a nutriceutical delivery system","volume":"2","author":"Takaiwa F.","year":"2007","journal-title":"CAB Rev. Perspect. Agri. Vet. Sci. Nutr. Nat. Resour."},{"key":"e_1_2_5_42_1","doi-asserted-by":"publisher","DOI":"10.4161\/hv.7.3.14302"},{"key":"e_1_2_5_43_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00425-009-0905-7"},{"key":"e_1_2_5_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2004.03.024"},{"key":"e_1_2_5_45_1","doi-asserted-by":"publisher","DOI":"10.2332\/allergolint.O-07-495"},{"key":"e_1_2_5_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0952-7915(02)00402-8"},{"key":"e_1_2_5_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2007.01.025"},{"key":"e_1_2_5_48_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-immunol-030409-101218"},{"key":"e_1_2_5_49_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0912181107"},{"key":"e_1_2_5_50_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1467-7652.2006.00199.x"},{"key":"e_1_2_5_51_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00299-007-0373-0"},{"key":"e_1_2_5_52_1","first-page":"101","volume-title":"Modification of Seed Composition to Promote Health and Nutrition","author":"Wakasa Y.","year":"2009"},{"key":"e_1_2_5_53_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1467-7652.2007.00287.x"},{"key":"e_1_2_5_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/0091-6749(83)90550-X"}],"container-title":["Plant Biotechnology Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fpbi.12007","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/pbi.12007","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,22]],"date-time":"2023-11-22T11:57:18Z","timestamp":1700654238000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/pbi.12007"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,10,16]]},"references-count":53,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2013,1]]}},"alternative-id":["10.1111\/pbi.12007"],"URL":"https:\/\/doi.org\/10.1111\/pbi.12007","archive":["Portico"],"relation":{},"ISSN":["1467-7644","1467-7652"],"issn-type":[{"value":"1467-7644","type":"print"},{"value":"1467-7652","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,10,16]]},"assertion":[{"value":"2012-07-29","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2012-09-07","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2012-10-16","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}