{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T04:52:19Z","timestamp":1784868739014,"version":"3.55.0"},"reference-count":27,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2013,3,27]],"date-time":"2013-03-27T00:00:00Z","timestamp":1364342400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Plant Biotechnology Journal"],"published-print":{"date-parts":[[2013,5]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Antibiotic and herbicide resistance genes are currently the most frequently used selectable marker genes for plant research and crop development. However, the use of antibiotics and herbicides must be carefully controlled because the degree of susceptibility to these compounds varies widely among plant species and because they can also affect plant regeneration. Therefore, new selectable marker systems that are effective for a broad range of plant species are still needed. Here, we report a simple and inexpensive system based on providing transgenic plant cells the capacity to convert a nonmetabolizable compound (phosphite, <jats:styled-content style=\"fixed-case\">P<\/jats:styled-content>hi) into an essential nutrient for cell growth (phosphate) trough the expression of a bacterial gene encoding a phosphite oxidoreductase (<jats:styled-content style=\"fixed-case\">PTXD<\/jats:styled-content>). This system is effective for the selection of <jats:italic>Arabidopsis<\/jats:italic> transgenic plants by germinating <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>0 seeds directly on media supplemented with <jats:styled-content style=\"fixed-case\">P<\/jats:styled-content>hi and to select transgenic tobacco shoots from cocultivated leaf disc explants using nutrient media supplemented with <jats:styled-content style=\"fixed-case\">P<\/jats:styled-content>hi as both a source of phosphorus and selective agent. Because the <jats:italic>ptx<jats:styled-content style=\"fixed-case\">D<\/jats:styled-content><\/jats:italic>\/<jats:styled-content style=\"fixed-case\">P<\/jats:styled-content>hi system also allows the establishment of large\u2010scale screening systems under greenhouse conditions completely eliminating false transformation events, it should facilitate the development of novel plant transformation methods.<\/jats:p>","DOI":"10.1111\/pbi.12063","type":"journal-article","created":{"date-parts":[[2013,3,27]],"date-time":"2013-03-27T10:24:37Z","timestamp":1364379877000},"page":"516-525","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["A novel dominant selectable system for the selection of transgenic plants under <i>in vitro<\/i> and greenhouse conditions based on phosphite metabolism"],"prefix":"10.1111","volume":"11","author":[{"given":"Damar L.","family":"L\u00f3pez\u2010Arredondo","sequence":"first","affiliation":[{"name":"Laboratorio Nacional de Gen\u00f3mica para la Biodiversidad Centro de Investigaci\u00f3n y de Estudios Avanzados del Instituto Polit\u00e9cnico Nacional Irapuato Guanajuato M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luis","family":"Herrera\u2010Estrella","sequence":"additional","affiliation":[{"name":"Laboratorio Nacional de Gen\u00f3mica para la Biodiversidad Centro de Investigaci\u00f3n y de Estudios Avanzados del Instituto Polit\u00e9cnico Nacional Irapuato Guanajuato M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,3,27]]},"reference":[{"key":"e_1_2_6_2_1","first-page":"646","article-title":"Phosphite supply affects phosphorus nutrition and biochemical responses in maize plants","volume":"5","author":"\u00c1vila F.W.","year":"2011","journal-title":"Aust J Crop 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