{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T15:44:19Z","timestamp":1781711059380,"version":"3.54.5"},"reference-count":53,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2013,8,23]],"date-time":"2013-08-23T00:00:00Z","timestamp":1377216000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Plant Biotechnology Journal"],"published-print":{"date-parts":[[2013,12]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Salicylic acid plays a critical role in activating plant defence responses after pathogen attack. Salicylic acid methyltransferase (SAMT) modulates the level of salicylic acid by converting salicylic acid to methyl salicylate. Here, we report that a <jats:italic>SAMT<\/jats:italic> gene from soybean (<jats:italic>GmSAMT1<\/jats:italic>) plays a role in soybean defence against soybean cyst nematode (<jats:italic>Heterodera glycines<\/jats:italic> Ichinohe, SCN). <jats:italic>GmSAMT1<\/jats:italic> was identified as a candidate SCN defence\u2010related gene in our previous analysis of soybean defence against SCN using GeneChip microarray experiments. The current study started with the isolation of the full\u2010length <jats:styled-content style=\"fixed-case\">cDNA<\/jats:styled-content>s of <jats:italic>GmSAMT1<\/jats:italic> from a SCN\u2010resistant soybean line and from a SCN\u2010susceptible soybean line. The two <jats:styled-content style=\"fixed-case\">cDNA<\/jats:styled-content>s encode proteins of identical sequences. The <jats:italic>GmSAMT1 <\/jats:italic><jats:styled-content style=\"fixed-case\">cDNA<\/jats:styled-content> was expressed in <jats:italic>Escherichia coli<\/jats:italic>. Using <jats:italic>in vitro<\/jats:italic> enzyme assays, <jats:italic>E.\u00a0coli<\/jats:italic>\u2010expressed GmSAMT1 was confirmed to function as salicylic acid methyltransferase. The apparent Km value of GmSAMT1 for salicylic acid was approximately 46\u00a0\u03bc<jats:sc>m<\/jats:sc>. To determine the role of <jats:italic>GmSAMT1<\/jats:italic> in soybean defence against SCN, transgenic hairy roots overexpressing <jats:italic>GmSAMT1<\/jats:italic> were produced and tested for SCN resistance. Overexpression of <jats:italic>GmSAMT1<\/jats:italic> in SCN\u2010susceptible backgrounds significantly reduced the development of SCN, indicating that overexpression of <jats:italic>GmSAMT1<\/jats:italic> in the transgenic hairy root system could confer resistance to SCN. Overexpression of <jats:italic>GmSAMT1<\/jats:italic> in transgenic hairy roots was also found to affect the expression of selected genes involved in salicylic acid biosynthesis and salicylic acid signal transduction.<\/jats:p>","DOI":"10.1111\/pbi.12108","type":"journal-article","created":{"date-parts":[[2013,8,23]],"date-time":"2013-08-23T05:00:57Z","timestamp":1377234057000},"page":"1135-1145","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":84,"title":["Overexpression of a soybean salicylic acid methyltransferase gene confers resistance to soybean cyst nematode"],"prefix":"10.1111","volume":"11","author":[{"given":"Jingyu","family":"Lin","sequence":"first","affiliation":[{"name":"Department of Plant Sciences University of Tennessee Knoxville TN USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mitra","family":"Mazarei","sequence":"additional","affiliation":[{"name":"Department of Plant Sciences University of Tennessee Knoxville TN USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nan","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Plant Sciences University of Tennessee Knoxville TN USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junwei J.","family":"Zhu","sequence":"additional","affiliation":[{"name":"Agroecosystem Management Research Unit USDA\u2010ARS Lincoln NE USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaofeng","family":"Zhuang","sequence":"additional","affiliation":[{"name":"Department of Plant Sciences University of Tennessee Knoxville TN USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wusheng","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Plant Sciences University of Tennessee Knoxville TN USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vincent R.","family":"Pantalone","sequence":"additional","affiliation":[{"name":"Department of Plant Sciences University of Tennessee Knoxville TN USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Prakash R.","family":"Arelli","sequence":"additional","affiliation":[{"name":"Crop Genetics Research Unit USDA\u2010ARS Jackson TN USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"Jr","given":"Charles N.","family":"Stewart","sequence":"additional","affiliation":[{"name":"Department of Plant Sciences University of Tennessee Knoxville TN USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Plant Sciences University of Tennessee Knoxville TN USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,8,23]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0002680"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00425-006-0270-8"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.2135\/cropsci2000.403824x"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"e_1_2_6_6_1","first-page":"142","article-title":"An improved technique for clearing and staining plant tissue for detection of nematodes","volume":"15","author":"Byrd D.","year":"1983","journal-title":"J. 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