{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T19:47:19Z","timestamp":1789156039251,"version":"build-2803163510"},"reference-count":72,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2013,9,14]],"date-time":"2013-09-14T00:00:00Z","timestamp":1379116800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Plant Biotechnology Journal"],"published-print":{"date-parts":[[2014,1]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Isoflavones, a group of flavonoids, restricted almost exclusively to family Leguminosae are known to exhibit anticancerous and anti\u2010osteoporotic activities in animal systems and have been a target for metabolic engineering in commonly consumed food crops. Earlier efforts based on the expression of legume isoflavone synthase (<jats:styled-content style=\"fixed-case\">IFS<\/jats:styled-content>) genes in nonlegume plant species led to the limited success in terms of isoflavone content in transgenic tissue due to the limitation of substrate for <jats:styled-content style=\"fixed-case\">IFS<\/jats:styled-content> enzyme. In this work to overcome this limitation, the activation of multiple genes of flavonoid pathway using <jats:italic>Arabidopsis<\/jats:italic> transcription factor <jats:italic>At<jats:styled-content style=\"fixed-case\">MYB<\/jats:styled-content>12<\/jats:italic> has been carried out. We developed transgenic tobacco lines constitutively co\u2010expressing <jats:italic>At<jats:styled-content style=\"fixed-case\">MYB<\/jats:styled-content>12<\/jats:italic> and <jats:italic>Gm<jats:styled-content style=\"fixed-case\">IFS<\/jats:styled-content>1<\/jats:italic> (soybean <jats:italic><jats:styled-content style=\"fixed-case\">IFS<\/jats:styled-content><\/jats:italic>) genes or independently and carried out their phytochemical and molecular analyses. The leaves of co\u2010expressing transgenic lines were found to have elevated flavonol content along with the accumulation of substantial amount of genistein glycoconjugates being at the highest levels that could be engineered in tobacco leaves till date. Oestrogen\u2010deficient (ovariectomized, Ovx) mice fed with leaf extract from transgenic plant co\u2010expressing <jats:italic>At<jats:styled-content style=\"fixed-case\">MYB<\/jats:styled-content>12<\/jats:italic> and <jats:italic>Gm<jats:styled-content style=\"fixed-case\">IFS<\/jats:styled-content>1<\/jats:italic> but not wild\u2010type extract exhibited significant conservation of trabecular microarchitecture, reduced osteoclast number and expression of osteoclastogenic genes, higher total serum antioxidant levels and increased uterine oestrogenicity compared with Ovx mice treated with vehicle (control). The skeletal effect of the transgenic extract was comparable to oestrogen\u2010treated Ovx mice. Together, our results establish an efficient strategy for successful pathway engineering of isoflavones and other flavonoids in crop plants and provide a direct evidence of improved osteoprotective effect of transgenic plant extract.<\/jats:p>","DOI":"10.1111\/pbi.12118","type":"journal-article","created":{"date-parts":[[2013,10,8]],"date-time":"2013-10-08T14:01:05Z","timestamp":1381240865000},"page":"69-80","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":102,"title":["Co\u2010expression of <i>Arabidopsis<\/i> transcription factor, <i>At<scp>MYB<\/scp>12<\/i>, and soybean isoflavone synthase, <i>Gm<scp>IFS<\/scp>1<\/i>, genes in tobacco leads to enhanced biosynthesis of isoflavones and flavonols resulting in osteoprotective activity"],"prefix":"10.1111","volume":"12","author":[{"given":"Ashutosh","family":"Pandey","sequence":"first","affiliation":[{"name":"Council of Scientific and Industrial Research\u2010National Botanical Research Institute (CSIR\u2010NBRI) Lucknow India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Prashant","family":"Misra","sequence":"additional","affiliation":[{"name":"Council of Scientific and Industrial Research\u2010National Botanical Research Institute (CSIR\u2010NBRI) Lucknow India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohd P.","family":"Khan","sequence":"additional","affiliation":[{"name":"CSIR\u2010Central Drug Research Institute (CSIR\u2010CDRI) Endocrinology Division and Center for Research in Anabolic Skeletal Targets in Health and Illness (ASTHI) Jankipuram Extension Lucknow India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gaurav","family":"Swarnkar","sequence":"additional","affiliation":[{"name":"CSIR\u2010Central Drug Research Institute (CSIR\u2010CDRI) Endocrinology Division and Center for Research in Anabolic Skeletal Targets in Health and Illness (ASTHI) Jankipuram Extension Lucknow India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mahesh C.","family":"Tewari","sequence":"additional","affiliation":[{"name":"CSIR\u2010Central Drug Research Institute (CSIR\u2010CDRI) Endocrinology Division and Center for Research in Anabolic Skeletal Targets in Health and Illness (ASTHI) Jankipuram Extension Lucknow India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sweta","family":"Bhambhani","sequence":"additional","affiliation":[{"name":"Council of Scientific and Industrial Research\u2010National Botanical Research Institute (CSIR\u2010NBRI) Lucknow India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ritu","family":"Trivedi","sequence":"additional","affiliation":[{"name":"CSIR\u2010Central Drug Research Institute (CSIR\u2010CDRI) Endocrinology Division and Center for Research in Anabolic Skeletal Targets in Health and Illness (ASTHI) Jankipuram Extension Lucknow India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naibedya","family":"Chattopadhyay","sequence":"additional","affiliation":[{"name":"CSIR\u2010Central Drug Research Institute (CSIR\u2010CDRI) Endocrinology Division and Center for Research in Anabolic Skeletal Targets in Health and Illness (ASTHI) Jankipuram Extension Lucknow India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Prabodh K.","family":"Trivedi","sequence":"additional","affiliation":[{"name":"Council of Scientific and Industrial Research\u2010National Botanical Research Institute (CSIR\u2010NBRI) Lucknow India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,9,14]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1271\/bbb.64.2276"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.104.056747"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0163-7258(01)00137-1"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2004-1677"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bone.2006.04.017"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.1506"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phytochem.2004.03.005"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1359\/jbmr.071031"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/ern046"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-08-091283-7.00030-8"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0031-9422(02)00116-4"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.arplant.55.031903.141729"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.107.108431"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.copbio.2008.02.002"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.2217\/nnm.12.171"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/erq442"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1359\/jbmr.2000.15.11.2251"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.227.4691.1229"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2007.13.9964"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pbi.2005.03.014"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/72671"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(97)01061-3"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/mnfr.201200200"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/cddis.2013.294"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200900666"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejpb.2012.08.001"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI200318859"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.arplant.57.032905.105252"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.212522099"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymben.2006.08.003"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-313X.2008.03597.x"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.2478\/v10102-009-0021-3"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-8741(02)00135-6"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00299-010-0861-5"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.109.150979"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1080\/10826076.2011.578324"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.2037"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1111\/pbi.12064"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.5650110802"},{"key":"e_1_2_6_41_1","doi-asserted-by":"crossref","first-page":"602","DOI":"10.1097\/gme.0b013e3181d0f7f0","article-title":"Total extract and standardized fraction from the stem bark of Buteamonosperma have osteoprotective action: evidence for the nonestrogenic osteogenic effect of the standardized fraction","volume":"17","author":"Pandey R.","year":"2010","journal-title":"Menopause"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00299-012-1300-6"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1080\/10826076.2011.636854"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(91)91423-A"},{"key":"e_1_2_6_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jnutbio.2005.06.002"},{"key":"e_1_2_6_46_1","doi-asserted-by":"publisher","DOI":"10.3109\/07388559609146599"},{"key":"e_1_2_6_47_1","first-page":"118","article-title":"Soy isoflavones modulate immune function in healthy postmenopausal women","volume":"83","author":"Ryan\u2010Borchers T.A.","year":"2006","journal-title":"Am. J. Clin. Nutr."},{"key":"e_1_2_6_48_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.107.096503"},{"key":"e_1_2_6_49_1","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.2546"},{"key":"e_1_2_6_50_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.434"},{"key":"e_1_2_6_51_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf800423u"},{"key":"e_1_2_6_52_1","doi-asserted-by":"publisher","DOI":"10.1093\/pcp\/pcm125"},{"key":"e_1_2_6_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2010.03.024"},{"key":"e_1_2_6_54_1","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/519387"},{"key":"e_1_2_6_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2012.12.021"},{"key":"e_1_2_6_56_1","doi-asserted-by":"publisher","DOI":"10.1006\/abbi.1999.1238"},{"key":"e_1_2_6_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phytochem.2007.05.017"},{"key":"e_1_2_6_58_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tplants.2007.06.006"},{"key":"e_1_2_6_59_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.2011.01637.x"},{"key":"e_1_2_6_60_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00425-006-0233-0"},{"key":"e_1_2_6_61_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2008.02.027"},{"key":"e_1_2_6_62_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2009.01.011"},{"key":"e_1_2_6_63_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2010.05.016"},{"key":"e_1_2_6_64_1","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.59.1.124-142.1995"},{"key":"e_1_2_6_65_1","doi-asserted-by":"publisher","DOI":"10.1039\/b511238a"},{"key":"e_1_2_6_66_1","doi-asserted-by":"publisher","DOI":"10.1002\/biot.200700084"},{"key":"e_1_2_6_67_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-2952(02)01445-4"},{"key":"e_1_2_6_68_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1211383110"},{"key":"e_1_2_6_69_1","doi-asserted-by":"publisher","DOI":"10.1016\/8756-3282(87)90012-3"},{"key":"e_1_2_6_70_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.arplant.55.031903.141714"},{"key":"e_1_2_6_71_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.126.2.485"},{"key":"e_1_2_6_72_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-313X.2010.04214.x"},{"key":"e_1_2_6_73_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.124.2.781"}],"container-title":["Plant Biotechnology Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fpbi.12118","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/pbi.12118","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,2]],"date-time":"2023-10-02T02:33:31Z","timestamp":1696214011000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/pbi.12118"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,9,14]]},"references-count":72,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2014,1]]}},"alternative-id":["10.1111\/pbi.12118"],"URL":"https:\/\/doi.org\/10.1111\/pbi.12118","archive":["Portico"],"relation":{},"ISSN":["1467-7644","1467-7652"],"issn-type":[{"value":"1467-7644","type":"print"},{"value":"1467-7652","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,9,14]]},"assertion":[{"value":"2013-05-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-08-09","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-09-14","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}