{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T14:20:47Z","timestamp":1784730047169,"version":"3.55.0"},"reference-count":48,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2013,12,27]],"date-time":"2013-12-27T00:00:00Z","timestamp":1388102400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Plant Biotechnology Journal"],"published-print":{"date-parts":[[2014,5]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Wheat yellow mosaic virus (<jats:styled-content style=\"fixed-case\">WYMV<\/jats:styled-content>) has spread rapidly and causes serious yield losses in the major wheat\u2010growing areas in China. Because it is vectored by the fungus\u2010like organism <jats:italic><jats:styled-content style=\"fixed-case\">P<\/jats:styled-content>olymyxa graminis<\/jats:italic> that survives for long periods in soil, it is difficult to eliminate by conventional crop management or fungicides. There is also only limited resistance in commercial cultivars. In this research, fourteen independent transgenic events were obtained by co\u2010transformation with the antisense <jats:italic><jats:styled-content style=\"fixed-case\">NI<\/jats:styled-content>b8<\/jats:italic> gene (the <jats:italic><jats:styled-content style=\"fixed-case\">NI<\/jats:styled-content>b replicase<\/jats:italic> of <jats:styled-content style=\"fixed-case\">WYMV<\/jats:styled-content>) and a selectable gene <jats:italic>bar<\/jats:italic>. Four original transgenic lines (N12, N13, N14 and N15) and an offspring line (N12\u20101) showed high and durable resistance to <jats:styled-content style=\"fixed-case\">WYMV<\/jats:styled-content> in the field. Four resistant lines were shown to have segregated and only contain <jats:italic><jats:styled-content style=\"fixed-case\">NI<\/jats:styled-content>b8<\/jats:italic> (without <jats:italic>bar<\/jats:italic>) by <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> and herbicide resistance testing in the later generations. Line N12\u20101 showed broad\u2010spectrum resistance to <jats:styled-content style=\"fixed-case\">WYMV<\/jats:styled-content> isolates from different sites in China. After growing in the infested soil, <jats:styled-content style=\"fixed-case\">WYMV<\/jats:styled-content> could not be detected by tissue printing and Western blot assays of transgenic wheat. The grain yield of transgenic wheat was about 10% greater than the wild\u2010type susceptible control. Northern blot and small <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> deep sequencing analyses showed that there was no accumulation of small interfering <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s targeting the <jats:italic><jats:styled-content style=\"fixed-case\">NI<\/jats:styled-content>b8<\/jats:italic> gene in transgenic wheat plants, suggesting that transgene <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> silencing, a common mechanism of virus\u2010derived disease resistance, is not involved in the process of <jats:styled-content style=\"fixed-case\">WYMV<\/jats:styled-content> resistance. This durable and broad\u2010spectrum resistance to <jats:styled-content style=\"fixed-case\">WYMV<\/jats:styled-content> in transgenic wheat will be useful for alleviating the damage caused by <jats:styled-content style=\"fixed-case\">WYMV<\/jats:styled-content>.<\/jats:p>","DOI":"10.1111\/pbi.12151","type":"journal-article","created":{"date-parts":[[2013,12,28]],"date-time":"2013-12-28T05:04:48Z","timestamp":1388207088000},"page":"447-456","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":39,"title":["Durable field resistance to wheat yellow mosaic virus in transgenic wheat containing the antisense virus polymerase gene"],"prefix":"10.1111","volume":"12","author":[{"given":"Ming","family":"Chen","sequence":"first","affiliation":[{"name":"Institute of Crop Sciences Chinese Academy of Agricultural Sciences\/National Key Facility for Crop Gene Resources and Genetic Improvement Key Laboratory of Biology and Genetic Improvement of Triticeae Crops Ministry of Agriculture Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liying","family":"Sun","sequence":"additional","affiliation":[{"name":"State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control MoA Key Laboratory for Plant Protection and Biotechnology Zhejiang Provincial Key Laboratory of Plant Virology Institute of Virology and Biotechnology Zhejiang Academy of Agricultural Sciences Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongya","family":"Wu","sequence":"additional","affiliation":[{"name":"Institute of Agricultural Sciences of Lixiahe Districts Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiong","family":"Chen","sequence":"additional","affiliation":[{"name":"State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control MoA Key Laboratory for Plant Protection and Biotechnology Zhejiang Provincial Key Laboratory of Plant Virology Institute of Virology and Biotechnology Zhejiang Academy of Agricultural Sciences Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Youzhi","family":"Ma","sequence":"additional","affiliation":[{"name":"Institute of Crop Sciences Chinese Academy of Agricultural Sciences\/National Key Facility for Crop Gene Resources and Genetic Improvement Key Laboratory of Biology and Genetic Improvement of Triticeae Crops Ministry of Agriculture Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoxiang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Institute of Agricultural Sciences of Lixiahe Districts Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lipu","family":"Du","sequence":"additional","affiliation":[{"name":"Institute of Crop Sciences Chinese Academy of Agricultural Sciences\/National Key Facility for Crop Gene Resources and Genetic Improvement Key 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