{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T00:55:56Z","timestamp":1789692956060,"version":"4.0.1"},"reference-count":41,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2014,6,29]],"date-time":"2014-06-29T00:00:00Z","timestamp":1404000000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R21 AI101329"],"award-info":[{"award-number":["R21 AI101329"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["U01 AI075549"],"award-info":[{"award-number":["U01 AI075549"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Plant Biotechnology Journal"],"published-print":{"date-parts":[[2014,10]]},"abstract":"Summary<\/jats:title>Previously, our group engineered a plant\u2010derived monoclonal antibody (MA<\/jats:styled-content>b pE<\/jats:styled-content>16) that efficiently treated West Nile virus (WNV<\/jats:styled-content>) infection in mice. In this study, we developed a pE<\/jats:styled-content>16 variant consisting of a single\u2010chain variable fragment (scF<\/jats:styled-content>v) fused to the heavy chain constant domains (CH<\/jats:sub><\/jats:styled-content>) of human I<\/jats:styled-content>gG<\/jats:styled-content> (pE<\/jats:styled-content>16scF<\/jats:styled-content>v\u2010CH<\/jats:sub><\/jats:styled-content>). pE<\/jats:styled-content>16 and pE<\/jats:styled-content>16scF<\/jats:styled-content>v\u2010CH<\/jats:sub><\/jats:styled-content> were expressed and assembled efficiently in Nicotiana benthamiana<\/jats:italic> \u2206XF<\/jats:styled-content> plants, a glycosylation mutant lacking plant\u2010specific N\u2010glycan residues. Glycan analysis revealed that \u2206XF<\/jats:styled-content> plant\u2010derived pE<\/jats:styled-content>16scF<\/jats:styled-content>v\u2010CH<\/jats:sub><\/jats:styled-content> (\u2206XF<\/jats:styled-content>pE16scF<\/jats:styled-content>v\u2010CH<\/jats:sub><\/jats:styled-content>) and pE<\/jats:styled-content>16 (\u2206XF<\/jats:styled-content>pE<\/jats:styled-content>16) both displayed a mammalian glycosylation profile. \u2206XF<\/jats:styled-content>pE<\/jats:styled-content>16 and \u2206XF<\/jats:styled-content>pE<\/jats:styled-content>16scF<\/jats:styled-content>v\u2010CH<\/jats:sub><\/jats:styled-content> demonstrated equivalent antigen\u2010binding affinity and kinetics, and slightly enhanced neutralization of WNV <\/jats:styled-content>in vitro<\/jats:italic> compared with the parent mammalian cell\u2010produced E16 (mE<\/jats:styled-content>16). A single dose of \u2206XF<\/jats:styled-content>pE<\/jats:styled-content>16 or \u2206XF<\/jats:styled-content>pE<\/jats:styled-content>16scF<\/jats:styled-content>v\u2010CH<\/jats:sub><\/jats:styled-content> protected mice against WNV<\/jats:styled-content>\u2010induced mortality even 4\u00a0days after infection at equivalent rates as mE<\/jats:styled-content>16. This study provides a detailed tandem comparison of the expression, structure and function of a therapeutic MA<\/jats:styled-content>b and its single\u2010chain variant produced in glycoengineered plants. Moreover, it demonstrates the development of anti\u2010WNV MA<\/jats:styled-content>b therapeutic variants that are equivalent in efficacy to pE<\/jats:styled-content>16, simpler to produce, and likely safer to use as therapeutics due to their mammalian N\u2010glycosylation. This platform may lead to a more robust and cost\u2010effective production of antibody\u2010based therapeutics against WNV<\/jats:styled-content> infection and other infectious, inflammatory or neoplastic diseases.<\/jats:p>","DOI":"10.1111\/pbi.12217","type":"journal-article","created":{"date-parts":[[2014,6,30]],"date-time":"2014-06-30T03:37:49Z","timestamp":1404099469000},"page":"1098-1107","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":60,"title":["Structural and functional characterization of an anti\u2010West Nile virus monoclonal antibody and its single\u2010chain variant produced in glycoengineered plants"],"prefix":"10.1111","volume":"12","author":[{"given":"Huafang","family":"Lai","sequence":"first","affiliation":[{"name":"The Biodesign Institute Arizona State University Tempe AZ USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junyun","family":"He","sequence":"additional","affiliation":[{"name":"The Biodesign Institute Arizona State University Tempe AZ USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan","family":"Hurtado","sequence":"additional","affiliation":[{"name":"The Biodesign Institute Arizona State University Tempe AZ USA"},{"name":"School of Life Sciences Arizona State University Tempe AZ USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jake","family":"Stahnke","sequence":"additional","affiliation":[{"name":"The Biodesign Institute Arizona State University Tempe AZ USA"},{"name":"School of Life Sciences Arizona State University Tempe AZ USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anja","family":"Fuchs","sequence":"additional","affiliation":[{"name":"Department of Medicine Washington University School of Medicine St. Louis MO USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erin","family":"Mehlhop","sequence":"additional","affiliation":[{"name":"Department of Pathology & Immunology Washington University School of Medicine St. Louis MO USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergey","family":"Gorlatov","sequence":"additional","affiliation":[{"name":"MacroGenics, Inc Rockville MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas","family":"Loos","sequence":"additional","affiliation":[{"name":"Department of Applied Genetics and Cell Biology University of Natural Resources and Applied Life Sciences Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael S.","family":"Diamond","sequence":"additional","affiliation":[{"name":"Department of Medicine Washington University School of Medicine St. Louis MO USA"},{"name":"MacroGenics, Inc Rockville MD USA"},{"name":"Department of Molecular Microbiology Washington University School of Medicine St. Louis MO USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1498-7013","authenticated-orcid":false,"given":"Qiang","family":"Chen","sequence":"additional","affiliation":[{"name":"The Biodesign Institute Arizona State University Tempe AZ USA"},{"name":"School of Life Sciences Arizona State University Tempe AZ USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,6,29]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.21120"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.2174\/1381612811319310006"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/biot.201200032"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.13031\/2013.26854"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1201\/b10952-5"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-7091-1419-3_8"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.4161\/hv.7.3.14262"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/pat.1990.220010112"},{"key":"e_1_2_6_10_1","first-page":"1","volume":"2014","author":"Chen Q.","year":"2014","journal-title":"Plant\u2010made biologics. BioMed. Res. Int."},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1467-7652.2009.00494.x"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.77.24.12941-12949.2003"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2009.04.028"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0606631103"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1467-7652.2009.00497.x"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/106783"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0093541"},{"key":"e_1_2_6_18_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1155\/2014\/952865","article-title":"A plant\u2010produced antigen elicits potent immune responses against West Nile virus in mice","volume":"2014","author":"He J.","year":"2014","journal-title":"BioMed. Res. Int."},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M900540-MCP200"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.22299"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.22652"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00299-011-1196-6"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0914503107"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1467-7652.2011.00649.x"},{"key":"e_1_2_6_25_1","first-page":"e50521","article-title":"Efficient agroinfiltration of plants for high\u2010level transient expression of recombinant proteins","volume":"77","author":"Leuzinger K.","year":"2013","journal-title":"J. Vis. Exp."},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.21423"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.abb.2012.05.011"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.110.171330"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2009.09.003"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm1240"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00643-07"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2013.8042"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1467-7652.2011.00593.x"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2005.10.030"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2007.03.002"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.abb.2012.07.001"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2008.01.053"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200700968"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1467-7652.2008.00330.x"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109.014126"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/256135"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1108360108"}],"container-title":["Plant Biotechnology Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fpbi.12217","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/pbi.12217","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,29]],"date-time":"2023-09-29T18:52:30Z","timestamp":1696013550000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/pbi.12217"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,6,29]]},"references-count":41,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2014,10]]}},"alternative-id":["10.1111\/pbi.12217"],"URL":"https:\/\/doi.org\/10.1111\/pbi.12217","archive":["Portico"],"relation":{},"ISSN":["1467-7644","1467-7652"],"issn-type":[{"value":"1467-7644","type":"print"},{"value":"1467-7652","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,6,29]]},"assertion":[{"value":"2014-02-19","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-05-22","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-06-29","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}