{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T18:59:50Z","timestamp":1789757990588,"version":"4.0.1"},"reference-count":58,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2015,3,4]],"date-time":"2015-03-04T00:00:00Z","timestamp":1425427200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"TUBITAK","award":["109-O-661"],"award-info":[{"award-number":["109-O-661"]}]},{"name":"TUBITAK","award":["11-O-036"],"award-info":[{"award-number":["11-O-036"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Plant Biotechnology Journal"],"published-print":{"date-parts":[[2015,4]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    Opium poppy (\n                    <jats:italic>\n                      <jats:styled-content style=\"fixed-case\">P<\/jats:styled-content>\n                      apaver somniferum\n                    <\/jats:italic>\n                    ) is an important medicinal plant producing benzylisoquinoline alkaloids (\n                    <jats:styled-content style=\"fixed-case\">BIA<\/jats:styled-content>\n                    ). Micro\n                    <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>\n                    s (mi\n                    <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>\n                    s) are endogenous small\n                    <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>\n                    s (\n                    <jats:styled-content style=\"fixed-case\">sRNA<\/jats:styled-content>\n                    s) of approximately 21 nucleotides. They are noncoding, but regulate gene expression in eukaryotes. Although many studies have been conducted on the identification and functions of plant mi\n                    <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>\n                    , scarce researches on mi\n                    <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>\n                    regulation of alkaloid biosynthesis have been reported. In this study, a total of 316 conserved and 11 novel mi\n                    <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>\n                    s were identified in opium poppy using second\u2010generation sequencing and direct cloning. Tissue\u2010specific regulation of mi\n                    <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>\n                    expression was comparatively analysed by mi\n                    <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>\n                    microarray assays. A total of 232 mi\n                    <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>\n                    s were found to be differentially expressed among four tissues. Likewise, 1469 target transcripts were detected using\n                    <jats:italic>in silico<\/jats:italic>\n                    and experimental approaches. The Kyoto Encyclopedia of Genes and Genomes pathway analyses indicated that mi\n                    <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>\n                    putatively regulates carbohydrate metabolism and genetic\u2010information processing. Additionally, mi\n                    <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>\n                    target transcripts were mostly involved in response to stress against various factors and secondary\u2010metabolite biosynthesis processes. Target transcript identification analyses revealed that some of the mi\n                    <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>\n                    s might be involved in\n                    <jats:styled-content style=\"fixed-case\">BIA<\/jats:styled-content>\n                    biosynthesis, such as pso\u2010miR13, pso\u2010miR2161 and pso\u2010miR408. Additionally, three putatively mature mi\n                    <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>\n                    sequences were predicted to be targeting\n                    <jats:styled-content style=\"fixed-case\">BIA<\/jats:styled-content>\n                    \u2010biosynthesis genes.\n                  <\/jats:p>","DOI":"10.1111\/pbi.12346","type":"journal-article","created":{"date-parts":[[2015,3,4]],"date-time":"2015-03-04T05:07:18Z","timestamp":1425445638000},"page":"409-420","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":125,"title":["Regulation of the alkaloid biosynthesis by mi\n                    <scp>RNA<\/scp>\n                    in opium poppy"],"prefix":"10.1111","volume":"13","author":[{"given":"Hatice","family":"Boke","sequence":"first","affiliation":[{"name":"Department of Biology Faculty of Science Cankiri Karatekin University Cankiri Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Esma","family":"Ozhuner","sequence":"additional","affiliation":[{"name":"Department of Biology Faculty of Science Cankiri Karatekin University Cankiri Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mine","family":"Turktas","sequence":"additional","affiliation":[{"name":"Department of Biology Faculty of Science Cankiri Karatekin University Cankiri Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iskender","family":"Parmaksiz","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Genetics Faculty of Science Gaziosmanpasa University Tokat Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sebahattin","family":"Ozcan","sequence":"additional","affiliation":[{"name":"Department of Field Crops Faculty of Agriculture Ankara University Ankara Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Turgay","family":"Unver","sequence":"additional","affiliation":[{"name":"Department of Biology Faculty of Science Cankiri Karatekin University Cankiri Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,3,4]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.01206"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2005.04.004"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11103-009-9480-3"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.016238"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-313X.2007.03197.x"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.123547.111"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1088060"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkr319"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-313X.2012.05084.x"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11103-012-9913-2"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2229-10-252"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0050298"},{"key":"e_1_2_6_14_1","first-page":"1","article-title":"Plant microRNAs: new players in functional genomics","volume":"37","author":"Eldem V.","year":"2013","journal-title":"Turk. 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