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To identify novel microbe\u2010associated molecular pattern (<jats:styled-content style=\"fixed-case\">MAMP<\/jats:styled-content>)\u2010responsive<jats:italic>cis<\/jats:italic>\u2010regulatory sequences for synthetic promoter design, a combination of bioinformatics and experimental approaches was employed. One<jats:italic>cis<\/jats:italic>\u2010sequence was identified which confers strong<jats:styled-content style=\"fixed-case\">MAMP<\/jats:styled-content>\u2010responsive reporter gene activity with low background activity. The 35\u2010bp\u2010long<jats:italic>cis<\/jats:italic>\u2010sequence was identified in the promoter of the<jats:italic><jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>rabidopsis thaliana<jats:styled-content style=\"fixed-case\">DJ<\/jats:styled-content>1E<\/jats:italic>gene, a homologue of the human oncogene<jats:italic><jats:styled-content style=\"fixed-case\">DJ<\/jats:styled-content>1<\/jats:italic>. In this study, this<jats:italic>cis<\/jats:italic>\u2010sequence is shown to be a tripartite<jats:italic>cis<\/jats:italic>\u2010regulatory module (<jats:styled-content style=\"fixed-case\">CRM<\/jats:styled-content>). A synthetic promoter with four copies of the<jats:styled-content style=\"fixed-case\">CRM<\/jats:styled-content>linked to a minimal promoter increases<jats:styled-content style=\"fixed-case\">MAMP<\/jats:styled-content>\u2010responsive reporter gene expression compared to the wild\u2010type<jats:italic><jats:styled-content style=\"fixed-case\">DJ<\/jats:styled-content>1E<\/jats:italic>promoter. The<jats:styled-content style=\"fixed-case\">CRM<\/jats:styled-content>consists of two<jats:styled-content style=\"fixed-case\">WT<\/jats:styled-content>\u2010boxes (<jats:styled-content style=\"fixed-case\">GGACTTTT<\/jats:styled-content>and<jats:styled-content style=\"fixed-case\">GGACTTTG<\/jats:styled-content>) and a variant of the<jats:styled-content style=\"fixed-case\">GCC<\/jats:styled-content>\u2010box (<jats:styled-content style=\"fixed-case\">GCCACC<\/jats:styled-content>), all required for<jats:styled-content style=\"fixed-case\">MAMP<\/jats:styled-content>and salicylic acid (<jats:styled-content style=\"fixed-case\">SA<\/jats:styled-content>) responsivity. Yeast one\u2010hybrid screenings using a transcription factor (<jats:styled-content style=\"fixed-case\">TF<\/jats:styled-content>)\u2010only prey library identified two<jats:styled-content style=\"fixed-case\">AP<\/jats:styled-content>2\/<jats:styled-content style=\"fixed-case\">ERF<\/jats:styled-content>s,<jats:styled-content style=\"fixed-case\">ORA<\/jats:styled-content>59 and<jats:styled-content style=\"fixed-case\">ERF<\/jats:styled-content>10, interacting antagonistically with the<jats:styled-content style=\"fixed-case\">CRM<\/jats:styled-content>.<jats:styled-content style=\"fixed-case\">ORA<\/jats:styled-content>59 activates reporter gene activity and requires the consensus core sequence<jats:styled-content style=\"fixed-case\">GCCNCC<\/jats:styled-content>for gene expression activation.<jats:styled-content style=\"fixed-case\">ERF<\/jats:styled-content>10 down\u2010regulates<jats:styled-content style=\"fixed-case\">MAMP<\/jats:styled-content>\u2010responsive gene expression. No<jats:styled-content style=\"fixed-case\">TF<\/jats:styled-content>s interacting with the<jats:styled-content style=\"fixed-case\">WT<\/jats:styled-content>\u2010boxes<jats:styled-content style=\"fixed-case\">GGACTTTT<\/jats:styled-content>and<jats:styled-content style=\"fixed-case\">GGACTTTG<\/jats:styled-content>were selected in yeast one\u2010hybrid screenings with the<jats:styled-content style=\"fixed-case\">TF<\/jats:styled-content>\u2010only prey library. In transgenic<jats:italic>Arabidopsis<\/jats:italic>, the synthetic promoter confers strong and specific reporter gene activity in response to biotrophs and necrotrophs as well as<jats:styled-content style=\"fixed-case\">SA<\/jats:styled-content>.<\/jats:p>","DOI":"10.1111\/pbi.12357","type":"journal-article","created":{"date-parts":[[2015,3,27]],"date-time":"2015-03-27T22:24:05Z","timestamp":1427495045000},"page":"61-71","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Functional dissection of a strong and specific microbe\u2010associated molecular pattern\u2010responsive synthetic promoter"],"prefix":"10.1111","volume":"14","author":[{"given":"Mona","family":"Lehmeyer","sequence":"first","affiliation":[{"name":"Institut f\u00fcr Genetik Technische Universit\u00e4t Braunschweig Braunschweig Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Konstantin","family":"Kanofsky","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Genetik Technische Universit\u00e4t Braunschweig Braunschweig Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erik K. 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