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However, intravenous administration includes discomfort or pain and might cause local and systemic infections that may lead to low patient compliance. An orally administered drug has the potential to alleviate these problems. In this study, we describe the potential use of plant cells as a vehicle for the oral delivery of recombinant human <jats:styled-content style=\"fixed-case\">GCD<\/jats:styled-content> (pr<jats:styled-content style=\"fixed-case\">GCD<\/jats:styled-content>) expressed in carrot cells. The <jats:italic>in vitro<\/jats:italic> results demonstrate that the plant cells protect the recombinant protein in the gastric fluids and may enable absorption into the blood. Feeding experiments, with rat and pig as model animals, using carrot cells containing pr<jats:styled-content style=\"fixed-case\">GCD<\/jats:styled-content>, show that active recombinant pr<jats:styled-content style=\"fixed-case\">GCD<\/jats:styled-content> was found in the digestive tract and blood system and reached both, liver and spleen, the target organs in <jats:styled-content style=\"fixed-case\">GD<\/jats:styled-content>. These results demonstrate that the oral administration of proteins encapsulated in plant cells is feasible. Specifically, carrot cells containing recombinant human pr<jats:styled-content style=\"fixed-case\">GCD<\/jats:styled-content> can be used as an oral delivery system and are a feasible alternative to intravenous administration of <jats:styled-content style=\"fixed-case\">ERT<\/jats:styled-content> for <jats:styled-content style=\"fixed-case\">GD<\/jats:styled-content>.<\/jats:p>","DOI":"10.1111\/pbi.12366","type":"journal-article","created":{"date-parts":[[2015,4,2]],"date-time":"2015-04-02T20:57:55Z","timestamp":1428008275000},"page":"1033-1040","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":66,"title":["Plant\u2010based oral delivery of \u03b2\u2010glucocerebrosidase as an enzyme replacement therapy for Gaucher's disease"],"prefix":"10.1111","volume":"13","author":[{"given":"Yoseph","family":"Shaaltiel","sequence":"first","affiliation":[{"name":"Protalix Biotherapeutics Carmiel 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