{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T03:22:36Z","timestamp":1790738556721,"version":"4.1.0"},"reference-count":57,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2015,4,28]],"date-time":"2015-04-28T00:00:00Z","timestamp":1430179200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Changjiang Scholars and Innovative Research Team in University of China","award":["IRT13053"],"award-info":[{"award-number":["IRT13053"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Plant Biotechnology Journal"],"published-print":{"date-parts":[[2016,1]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    In plant cells, the vacuolar\u2010type H\n                    <jats:sup>+<\/jats:sup>\n                    \u2010\n                    <jats:styled-content style=\"fixed-case\">ATP<\/jats:styled-content>\n                    ases (V\u2010\n                    <jats:styled-content style=\"fixed-case\">ATP<\/jats:styled-content>\n                    ase) are localized in the tonoplast, Golgi,\n                    <jats:italic>trans<\/jats:italic>\n                    \u2010Golgi network and endosome. However, little is known about how V\u2010\n                    <jats:styled-content style=\"fixed-case\">ATP<\/jats:styled-content>\n                    ase influences plant growth, particularly with regard to the V\u2010\n                    <jats:styled-content style=\"fixed-case\">ATP<\/jats:styled-content>\n                    ase c subunit (\n                    <jats:styled-content style=\"fixed-case\">VHA<\/jats:styled-content>\n                    \u2010c). Here, we characterized the function of a\n                    <jats:italic>\n                      <jats:styled-content style=\"fixed-case\">VHA<\/jats:styled-content>\n                      \u2010c\n                    <\/jats:italic>\n                    gene from\n                    <jats:italic>Puccinellia tenuiflora<\/jats:italic>\n                    (Put\n                    <jats:styled-content style=\"fixed-case\">VHA<\/jats:styled-content>\n                    \u2010c) in plant growth. Compared to the wild\u2010type, transgenic plants overexpressing\n                    <jats:italic>\n                      Put\n                      <jats:styled-content style=\"fixed-case\">VHA<\/jats:styled-content>\n                      \u2010c\n                    <\/jats:italic>\n                    in\n                    <jats:italic>Arabidopsis thaliana<\/jats:italic>\n                    exhibit better growth phenotypes in root length, fresh weight, plant height and silique number under the normal and salt stress conditions due to noticeably higher V\u2010\n                    <jats:styled-content style=\"fixed-case\">ATP<\/jats:styled-content>\n                    ase activity. Consistently, the\n                    <jats:italic>Arabidopsis atvha\u2010c5<\/jats:italic>\n                    mutant shows reduced V\u2010\n                    <jats:styled-content style=\"fixed-case\">ATP<\/jats:styled-content>\n                    ase activity and retarded plant growth. Furthermore, confocal and immunogold electron microscopy assays demonstrate that Put\n                    <jats:styled-content style=\"fixed-case\">VHA<\/jats:styled-content>\n                    \u2010c is mainly localized to endosomal compartments. The treatment of concanamycin A (ConcA), a specific inhibitor of V\u2010\n                    <jats:styled-content style=\"fixed-case\">ATP<\/jats:styled-content>\n                    ases, leads to obvious aggregation of the endosomal compartments labelled with Put\n                    <jats:styled-content style=\"fixed-case\">VHA<\/jats:styled-content>\n                    \u2010c\u2010\n                    <jats:styled-content style=\"fixed-case\">GFP<\/jats:styled-content>\n                    . Moreover, ConcA treatment results in the abnormal localization of two plasma membrane (\n                    <jats:styled-content style=\"fixed-case\">PM<\/jats:styled-content>\n                    ) marker proteins Pinformed 1 (At\n                    <jats:styled-content style=\"fixed-case\">PIN<\/jats:styled-content>\n                    1) and regulator of G protein signalling\u20101 (At\n                    <jats:styled-content style=\"fixed-case\">RGS<\/jats:styled-content>\n                    1). These findings suggest that the decrease in V\u2010\n                    <jats:styled-content style=\"fixed-case\">ATP<\/jats:styled-content>\n                    ase activity blocks endosomal trafficking. Taken together, our results strongly suggest that the Put\n                    <jats:styled-content style=\"fixed-case\">VHA<\/jats:styled-content>\n                    \u2010c plays an important role in plant growth by influencing V\u2010\n                    <jats:styled-content style=\"fixed-case\">ATP<\/jats:styled-content>\n                    ase\u2010dependent endosomal trafficking.\n                  <\/jats:p>","DOI":"10.1111\/pbi.12381","type":"journal-article","created":{"date-parts":[[2015,4,28]],"date-time":"2015-04-28T02:41:26Z","timestamp":1430188886000},"page":"271-283","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":52,"title":["Conserved V\u2010\n                    <scp>ATP<\/scp>\n                    ase c subunit plays a role in plant growth by influencing V\u2010\n                    <scp>ATP<\/scp>\n                    ase\u2010dependent endosomal trafficking"],"prefix":"10.1111","volume":"14","author":[{"given":"Aimin","family":"Zhou","sequence":"first","affiliation":[{"name":"Key Laboratory of Saline\u2010alkali Vegetation Ecology Restoration in Oil Field (SAVER) Ministry of Education Alkali Soil Natural Environmental Science Center (ASNESC) Northeast Forestry University Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuanyuan","family":"Bu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Saline\u2010alkali Vegetation Ecology Restoration in Oil Field (SAVER) Ministry of Education Alkali Soil Natural Environmental Science Center (ASNESC) Northeast Forestry University Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tetsuo","family":"Takano","sequence":"additional","affiliation":[{"name":"Asian Natural Environmental Science Center The University of Tokyo Nishitokyo\u2010shi Tokyo 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