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The small <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> libraries were derived from root hairs inoculated with <jats:italic><jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>radyrhizobium japonicum<\/jats:italic> (In_<jats:styled-content style=\"fixed-case\">RH<\/jats:styled-content>) or mock\u2010inoculated with water (Un_<jats:styled-content style=\"fixed-case\">RH<\/jats:styled-content>), as well as from the comparable inoculated stripped root samples (i.e. inoculated roots with the root hairs removed). Sequencing of these libraries identified a total of 114 mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s, including 22 novel mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s. A comparison of mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> abundance among the 114 mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s identified 66 mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s that were differentially expressed between root hairs and stripped roots, and 48 mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s that were differentially regulated in infected root hairs in response to <jats:italic><jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>.\u00a0japonicum<\/jats:italic> when compared to uninfected root hairs (<jats:italic>P<\/jats:italic>\u00a0\u2264\u00a00.05). A parallel analysis of <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> ends (<jats:styled-content style=\"fixed-case\">PARE<\/jats:styled-content>) library was constructed and sequenced to reveal a total of 405 soybean mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> targets, with most predicted to encode transcription factors or proteins involved in protein modification, protein degradation and hormone pathways. The roles of gma\u2010miR4416 and gma\u2010miR2606b during nodulation were further analysed. Ectopic expression of these two mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s in soybean roots resulted in significant changes in nodule numbers. mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> target information suggested that gma\u2010miR2606b regulates a <jats:italic>Mannosyl\u2010oligosaccharide 1, 2\u2010alpha\u2010mannosidase<\/jats:italic> gene, while gma\u2010miR4416 regulates the expression of a <jats:italic>rhizobium\u2010induced peroxidase 1<\/jats:italic> (<jats:italic><jats:styled-content style=\"fixed-case\">RIP<\/jats:styled-content>1<\/jats:italic>)\u2010like peroxidase gene, <jats:italic>Gm<jats:styled-content style=\"fixed-case\">RIP<\/jats:styled-content>1<\/jats:italic>, during nodulation.<\/jats:p>","DOI":"10.1111\/pbi.12387","type":"journal-article","created":{"date-parts":[[2015,5,14]],"date-time":"2015-05-14T11:58:05Z","timestamp":1431604685000},"page":"332-341","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":49,"title":["Identification and functional characterization of soybean root hair micro<scp>RNA<\/scp>s expressed in response to <i><scp>B<\/scp>radyrhizobium japonicum<\/i> infection"],"prefix":"10.1111","volume":"14","author":[{"given":"Zhe","family":"Yan","sequence":"first","affiliation":[{"name":"Divisions of Plant Science and Biochemistry National Center for Soybean Biotechnology Christopher S. Bond Life Sciences Center University of Missouri Columbia MO USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Md Shakhawat","family":"Hossain","sequence":"additional","affiliation":[{"name":"Divisions of Plant Science and Biochemistry National Center for Soybean Biotechnology Christopher S. Bond Life Sciences Center University of Missouri Columbia MO USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oswaldo","family":"Vald\u00e9s\u2010L\u00f3pez","sequence":"additional","affiliation":[{"name":"Divisions of Plant Science and Biochemistry National Center for Soybean Biotechnology Christopher S. Bond Life Sciences Center University of Missouri Columbia MO USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nhung T.","family":"Hoang","sequence":"additional","affiliation":[{"name":"Divisions of Plant Science and Biochemistry National Center for Soybean Biotechnology Christopher S. Bond Life Sciences Center University of Missouri Columbia MO USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jixian","family":"Zhai","sequence":"additional","affiliation":[{"name":"Department of Plant and Soil Sciences and Delaware Biotechnology Institute University of Delaware Newark DE USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Wang","sequence":"additional","affiliation":[{"name":"Divisions of Plant Science and Biochemistry National Center for Soybean Biotechnology Christopher S. Bond Life Sciences Center University of Missouri Columbia MO USA"},{"name":"National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI) Institute of Crop Science Chinese Academy of Agricultural Sciences Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marc","family":"Libault","sequence":"additional","affiliation":[{"name":"Divisions of Plant Science and Biochemistry National Center for Soybean Biotechnology Christopher S. Bond Life Sciences Center University of Missouri Columbia MO USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurent","family":"Brechenmacher","sequence":"additional","affiliation":[{"name":"Divisions of Plant Science and Biochemistry National Center for Soybean Biotechnology Christopher S. 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