{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T23:12:54Z","timestamp":1790637174473,"version":"4.1.0"},"reference-count":42,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2015,8,24]],"date-time":"2015-08-24T00:00:00Z","timestamp":1440374400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002924","name":"Federaci\u00f3n Espa\u00f1ola de Enfermedades Raras","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002924","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Plant Biotechnology Journal"],"published-print":{"date-parts":[[2016,3]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Gluten proteins are responsible for the viscoelastic properties of wheat flour but also for triggering pathologies in susceptible individuals, of which coeliac disease (<jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>) and noncoeliac gluten sensitivity may affect up to 8% of the population. The only effective treatment for affected persons is a strict gluten\u2010free diet. Here, we report the effectiveness of seven plasmid combinations, encompassing <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>i fragments from \u03b1\u2010, \u03b3\u2010, \u03c9\u2010gliadins, and <jats:styled-content style=\"fixed-case\">LMW<\/jats:styled-content> glutenin subunits, for silencing the expression of different prolamin fractions. Silencing patterns of transgenic lines were analysed by gel electrophoresis, <jats:styled-content style=\"fixed-case\">RP<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">HPLC<\/jats:styled-content> and mass spectrometry (<jats:styled-content style=\"fixed-case\">LC<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content>\/<jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content>), whereas gluten immunogenicity was assayed by an anti\u2010gliadin 33\u2010mer monoclonal antibody (moAb). Plasmid combinations 1 and 2 downregulated only \u03b3\u2010 and \u03b1\u2010gliadins, respectively. Four plasmid combinations were highly effective in the silencing of \u03c9\u2010gliadins and \u03b3\u2010gliadins, and three of these also silenced \u03b1\u2010gliadins. <jats:styled-content style=\"fixed-case\">HMW<\/jats:styled-content> glutenins were upregulated in all but one plasmid combination, while <jats:styled-content style=\"fixed-case\">LMW<\/jats:styled-content> glutenins were downregulated in three plasmid combinations. Total protein and starch contents were unaffected regardless of the plasmid combination used. Six plasmid combinations provided strong reduction in the gluten content as measured by moAb and for two combinations, this reduction was higher than 90% in comparison with the wild type. <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content> epitope analysis in peptides identified in <jats:styled-content style=\"fixed-case\">LC<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content>\/<jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content> showed that lines from three plasmid combinations were totally devoid of <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content> epitopes from the highly immunogenic \u03b1\u2010 and \u03c9\u2010gliadins. Our findings raise the prospect of breeding wheat species with low levels of harmful gluten, and of achieving the important goal of developing nontoxic wheat cultivars.<\/jats:p>","DOI":"10.1111\/pbi.12455","type":"journal-article","created":{"date-parts":[[2015,8,24]],"date-time":"2015-08-24T15:05:56Z","timestamp":1440428756000},"page":"986-996","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":78,"title":["Targeting of prolamins by <scp>RNA<\/scp>i in bread wheat: effectiveness of seven silencing\u2010fragment combinations for obtaining lines devoid of coeliac disease epitopes from highly immunogenic gliadins"],"prefix":"10.1111","volume":"14","author":[{"given":"Francisco","family":"Barro","sequence":"first","affiliation":[{"name":"Departamento de Mejora Gen\u00e9tica Instituto de Agricultura Sostenible (IAS) Consejo Superior de Investigaciones Cient\u00edficas (CSIC) C\u00f3rdoba Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julio C. M.","family":"Iehisa","sequence":"additional","affiliation":[{"name":"Departamento de Mejora Gen\u00e9tica Instituto de Agricultura Sostenible (IAS) Consejo Superior de Investigaciones Cient\u00edficas (CSIC) C\u00f3rdoba Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda J.","family":"Gim\u00e9nez","sequence":"additional","affiliation":[{"name":"Departamento de Mejora Gen\u00e9tica Instituto de Agricultura Sostenible (IAS) Consejo Superior de Investigaciones Cient\u00edficas (CSIC) C\u00f3rdoba Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda D.","family":"Garc\u00eda\u2010Molina","sequence":"additional","affiliation":[{"name":"Departamento de Mejora Gen\u00e9tica Instituto de Agricultura Sostenible (IAS) Consejo Superior de Investigaciones Cient\u00edficas (CSIC) C\u00f3rdoba Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carmen V.","family":"Ozuna","sequence":"additional","affiliation":[{"name":"Departamento de Mejora Gen\u00e9tica Instituto de Agricultura Sostenible (IAS) Consejo Superior de Investigaciones Cient\u00edficas (CSIC) C\u00f3rdoba Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabel","family":"Comino","sequence":"additional","affiliation":[{"name":"Departamento de Microbiolog\u00eda y Parasitolog\u00eda Facultad de Farmacia Universidad de Sevilla Sevilla Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolina","family":"Sousa","sequence":"additional","affiliation":[{"name":"Departamento de Microbiolog\u00eda y Parasitolog\u00eda Facultad de Farmacia Universidad de Sevilla Sevilla Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Javier","family":"Gil\u2010Humanes","sequence":"additional","affiliation":[{"name":"Departamento de Mejora Gen\u00e9tica Instituto de Agricultura Sostenible (IAS) Consejo Superior de Investigaciones Cient\u00edficas (CSIC) C\u00f3rdoba Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,8,24]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-immunol-040210-092915"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.4161\/gmcr.2.1.15884"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10142-013-0321-8"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1053\/gast.2002.35381"},{"key":"e_1_2_6_6_1","first-page":"23","article-title":"Protein composition and techno\u2010functional properties of transgenic wheat with reduced \u03b1\u2010gliadin content obtained by RNA interference","volume":"85","author":"Becker D.","year":"2012","journal-title":"J. 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