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However, one of the main constraints for the cultivation of microalgae is the potential contamination with biological pollutants, such as bacteria, fungi, zooplankton or other undesirable microalgae. In closed bioreactors, the control of contamination requires the sterilization of the media, containers and all materials, which increases the cost of production, whereas open pond systems severely limits the number of species that can be cultivated under extreme environmental conditions to prevent contaminations. Here, we report the metabolic engineering of <jats:italic>Chlamydomonas reinhardtii<\/jats:italic> to use phosphite as its sole phosphorus source by expressing the <jats:italic>ptxD<\/jats:italic> gene from <jats:italic>Pseudomonas stutzeri<\/jats:italic> <jats:styled-content style=\"fixed-case\">WM<\/jats:styled-content>88, which encodes a phosphite oxidoreductase able to oxidize phosphite into phosphate using <jats:styled-content style=\"fixed-case\">NAD<\/jats:styled-content> as a cofactor. Engineered <jats:italic>C.\u00a0reinhardtii<\/jats:italic> lines are capable of becoming the dominant species in a mixed culture when fertilized with phosphite as a sole phosphorus source. Our results represent a new platform for the production of microalgae, potentially useful for both closed photobioreactors and open pond systems without the need for using sterile conditions nor antibiotics or herbicides to prevent contamination with biological pollutants.<\/jats:p>","DOI":"10.1111\/pbi.12564","type":"journal-article","created":{"date-parts":[[2016,3,23]],"date-time":"2016-03-23T13:56:37Z","timestamp":1458741397000},"page":"2066-2076","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":75,"title":["A novel genetic engineering platform for the effective management of biological contaminants for the production of microalgae"],"prefix":"10.1111","volume":"14","author":[{"given":"Maribel M.","family":"Loera\u2010Quezada","sequence":"first","affiliation":[{"name":"Laboratorio Nacional de Gen\u00f3mica para la Biodiversidad Unidad de Gen\u00f3mica Avanzada del Centro de Investigaci\u00f3n y de Estudios Avanzados del Instituto Polit\u00e9cnico Nacional Guanajuato M\u00e9xico"},{"name":"StelaGenomics M\u00e9xico S de RL de CV Guanajuato M\u00e9xico"},{"name":"Instituto de Ecolog\u00eda A.C. Xalapa, Veracruz Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marco Antonio","family":"Leyva\u2010Gonz\u00e1lez","sequence":"additional","affiliation":[{"name":"StelaGenomics M\u00e9xico S de RL de CV Guanajuato M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gilberto","family":"Vel\u00e1zquez\u2010Ju\u00e1rez","sequence":"additional","affiliation":[{"name":"StelaGenomics M\u00e9xico S de RL de CV Guanajuato M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lenin","family":"Sanchez\u2010Calder\u00f3n","sequence":"additional","affiliation":[{"name":"StelaGenomics M\u00e9xico S de RL de CV Guanajuato M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mauro","family":"Do Nascimento","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones en Biodiversidad y Biotecnolog\u00eda Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Damar","family":"L\u00f3pez\u2010Arredondo","sequence":"additional","affiliation":[{"name":"StelaGenomics M\u00e9xico S de RL de CV Guanajuato M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luis","family":"Herrera\u2010Estrella","sequence":"additional","affiliation":[{"name":"Laboratorio Nacional de Gen\u00f3mica para la Biodiversidad Unidad de Gen\u00f3mica Avanzada del Centro de Investigaci\u00f3n y de Estudios Avanzados del Instituto Polit\u00e9cnico Nacional Guanajuato M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,5,28]]},"reference":[{"key":"e_1_2_6_2_1","first-page":"713","article-title":"Effect of phosphite supply in nutrient solution on yield, phosphorus nutrition and enzymatic behavior in common bean (Phaseolus vulgaris L.) plants","volume":"7","author":"\u00c1vila F.W.","year":"2013","journal-title":"Aust. 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