{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,9]],"date-time":"2026-05-09T02:50:28Z","timestamp":1778295028444,"version":"3.51.4"},"reference-count":41,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2016,5,23]],"date-time":"2016-05-23T00:00:00Z","timestamp":1463961600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Tennessee Soybean Promotion Board"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Plant Biotechnology Journal"],"published-print":{"date-parts":[[2016,11]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Soybean (<jats:italic>Glycine max<\/jats:italic> (L.) Merr.) salicylic acid methyl transferase (Gm<jats:styled-content style=\"fixed-case\">SAMT<\/jats:styled-content>1) catalyses the conversion of salicylic acid to methyl salicylate. Prior results showed that when <jats:italic>Gm<jats:styled-content style=\"fixed-case\">SAMT<\/jats:styled-content>1<\/jats:italic> was overexpressed in transgenic soybean hairy roots, resistance is conferred against soybean cyst nematode (<jats:styled-content style=\"fixed-case\">SCN<\/jats:styled-content>), <jats:italic>Heterodera glycines<\/jats:italic> Ichinohe. In this study, we produced transgenic soybean overexpressing <jats:italic>Gm<jats:styled-content style=\"fixed-case\">SAMT<\/jats:styled-content>1<\/jats:italic> and characterized their response to various <jats:styled-content style=\"fixed-case\">SCN<\/jats:styled-content> races. Transgenic plants conferred a significant reduction in the development of <jats:styled-content style=\"fixed-case\">SCN HG<\/jats:styled-content> type 1.2.5.7 (race 2), <jats:styled-content style=\"fixed-case\">HG<\/jats:styled-content> type 0 (race 3) and <jats:styled-content style=\"fixed-case\">HG<\/jats:styled-content> type 2.5.7 (race 5). Among transgenic lines, <jats:italic>Gm<jats:styled-content style=\"fixed-case\">SAMT<\/jats:styled-content>1<\/jats:italic> expression in roots was positively associated with <jats:styled-content style=\"fixed-case\">SCN<\/jats:styled-content> resistance. In some transgenic lines, there was a significant decrease in salicylic acid titer relative to control plants. No significant seed yield differences were observed between transgenics and control soybean plants grown in one greenhouse with 22\u00a0\u00b0C day\/night temperature, whereas transgenic soybean had higher yield than controls grown a warmer greenhouse (27\u00a0\u00b0C day\/23\u00a0\u00b0C night) temperature. In a 1\u2010year field experiment in Knoxville, TN, there was no significant difference in seed yield between the transgenic and nontransgenic soybean under conditions with negligible <jats:styled-content style=\"fixed-case\">SCN<\/jats:styled-content> infection. We hypothesize that <jats:italic>Gm<jats:styled-content style=\"fixed-case\">SAMT<\/jats:styled-content>1<\/jats:italic> expression affects salicylic acid biosynthesis, which, in turn, attenuates <jats:styled-content style=\"fixed-case\">SCN<\/jats:styled-content> development, without negative consequences to soybean yield or other morphological traits. Thus, we conclude that <jats:italic>Gm<jats:styled-content style=\"fixed-case\">SAMT<\/jats:styled-content>1<\/jats:italic> overexpression confers broad resistance to multiple <jats:styled-content style=\"fixed-case\">SCN<\/jats:styled-content> races, which would be potentially applicable to commercial production.<\/jats:p>","DOI":"10.1111\/pbi.12566","type":"journal-article","created":{"date-parts":[[2016,4,11]],"date-time":"2016-04-11T05:12:43Z","timestamp":1460351563000},"page":"2100-2109","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":36,"title":["Transgenic soybean overexpressing <i>Gm<scp>SAMT<\/scp>1<\/i> exhibits resistance to multiple\u2010<scp>HG<\/scp> types of soybean cyst nematode <i>Heterodera glycines<\/i>"],"prefix":"10.1111","volume":"14","author":[{"given":"Jingyu","family":"Lin","sequence":"first","affiliation":[{"name":"Department of Plant Sciences University of Tennessee Knoxville TN USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mitra","family":"Mazarei","sequence":"additional","affiliation":[{"name":"Department of Plant Sciences University of Tennessee Knoxville TN USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nan","family":"Zhao","sequence":"additional","affiliation":[{"name":"Biosciences Division Oak Ridge National Laboratory Oak Ridge TN USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Catherine N.","family":"Hatcher","sequence":"additional","affiliation":[{"name":"Department of Plant Sciences University of Tennessee Knoxville TN USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wegi A.","family":"Wuddineh","sequence":"additional","affiliation":[{"name":"Department of Plant Sciences University of Tennessee Knoxville TN USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mary","family":"Rudis","sequence":"additional","affiliation":[{"name":"Department of Plant Sciences University of Tennessee Knoxville TN USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Timothy J.","family":"Tschaplinski","sequence":"additional","affiliation":[{"name":"Biosciences Division Oak Ridge National Laboratory Oak Ridge TN USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vincent R.","family":"Pantalone","sequence":"additional","affiliation":[{"name":"Department of Plant Sciences University of Tennessee Knoxville TN USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Prakash R.","family":"Arelli","sequence":"additional","affiliation":[{"name":"Crop Genetics Research Unit USDA\u2010ARS  Jackson TN USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tarek","family":"Hewezi","sequence":"additional","affiliation":[{"name":"Department of Plant Sciences University of Tennessee Knoxville TN USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Feng","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Plant Sciences University of Tennessee Knoxville TN USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"Jr","given":"Charles Neal","family":"Stewart","sequence":"additional","affiliation":[{"name":"Department of Plant Sciences University of Tennessee Knoxville TN 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