{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T18:36:05Z","timestamp":1791916565699,"version":"4.3.4"},"reference-count":40,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2017,4,21]],"date-time":"2017-04-21T00:00:00Z","timestamp":1492732800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"UK Biotechnology and Biosciences Research Council","award":["Designing Seeds"],"award-info":[{"award-number":["Designing Seeds"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Plant Biotechnology Journal"],"published-print":{"date-parts":[[2017,11]]},"abstract":"Summary<\/jats:title>Arabinoxylan (AX<\/jats:styled-content>) is the major component of the cell walls of wheat grain (70% in starchy endosperm), is an important determinant of end\u2010use qualities affecting food processing, use for animal feed and distilling and is a major source of dietary fibre in the human diet. AX<\/jats:styled-content> is a heterogeneous polysaccharide composed of fractions which can be sequentially extracted by water (WE<\/jats:styled-content>\u2010AX<\/jats:styled-content>), then xylanase action (XE<\/jats:styled-content>\u2010AX<\/jats:styled-content>) leaving an unextractable (XU<\/jats:styled-content>\u2010AX<\/jats:styled-content>) fraction. We determined arabinosylation and feruloylation of AX<\/jats:styled-content> in these fractions in both wild\u2010type wheat and RNA<\/jats:styled-content>i lines with decreased AX<\/jats:styled-content> content (TaGT<\/jats:styled-content>43_2 RNA<\/jats:styled-content>i, TaGT<\/jats:styled-content>47_2 RNA<\/jats:styled-content>i) or decreased arabinose 3\u2010linked to mono\u2010substituted xylose (TaXAT<\/jats:styled-content>1 RNA<\/jats:styled-content>i). We show that these fractions are characterized by the degree of feruloylation of AX<\/jats:styled-content>, <5, 5\u20137 and 13\u201319\u00a0mg bound ferulate (g\u22121<\/jats:sup> AX<\/jats:styled-content>), and their content of diferulates (diFA<\/jats:styled-content>), <0.3, 1\u20131.7 and 4\u20135\u00a0mg (g\u22121<\/jats:sup> AX<\/jats:styled-content>), for the WE<\/jats:styled-content>, XE<\/jats:styled-content> and XU<\/jats:styled-content> fractions, respectively, in all RNA<\/jats:styled-content>i lines and their control lines. The amount of AX<\/jats:styled-content> and its degree of arabinosylation and feruloylation were less affected by RNA<\/jats:styled-content>i transgenes in the XE<\/jats:styled-content>\u2010AX<\/jats:styled-content> fraction than in the WE<\/jats:styled-content>\u2010AX<\/jats:styled-content> fraction and largely unaffected in the XU<\/jats:styled-content>\u2010AX<\/jats:styled-content> fraction. As the majority of diFA<\/jats:styled-content> is associated with the XU<\/jats:styled-content>\u2010AX<\/jats:styled-content> fraction, there was only a small effect (TaGT<\/jats:styled-content>43_2 RNA<\/jats:styled-content>i, TaGT<\/jats:styled-content>47_2 RNA<\/jats:styled-content>i) or no effect (TaXAT<\/jats:styled-content>1 RNA<\/jats:styled-content>i) on total diFA<\/jats:styled-content> content. Our results are compatible with a model where, to maintain cell wall function, diFA<\/jats:styled-content> is maintained at stable levels when other AX<\/jats:styled-content> properties are altered.<\/jats:p>","DOI":"10.1111\/pbi.12727","type":"journal-article","created":{"date-parts":[[2017,3,19]],"date-time":"2017-03-19T05:52:12Z","timestamp":1489902732000},"page":"1429-1438","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":42,"title":["Feruloylation and structure of arabinoxylan in wheat endosperm cell walls from RNA<\/scp>i lines with suppression of genes responsible for backbone synthesis and decoration"],"prefix":"10.1111","volume":"15","author":[{"given":"Jackie","family":"Freeman","sequence":"first","affiliation":[{"name":"Plant Biology and Crop Science Rothamsted Research Harpenden Hertfordshire UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jane 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Harpenden Hertfordshire UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1412-8828","authenticated-orcid":false,"given":"Rowan A.C.","family":"Mitchell","sequence":"additional","affiliation":[{"name":"Plant Biology and Crop Science Rothamsted Research Harpenden Hertfordshire 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