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Vaccination remains the single most effective weapon in combatting <jats:styled-content style=\"fixed-case\">AHS<\/jats:styled-content>, as there is no treatment for the disease apart from good animal husbandry. However, the only commercially available vaccine is a live\u2010attenuated version of the virus (<jats:styled-content style=\"fixed-case\">LAV<\/jats:styled-content>). The threat of outbreaks of the disease outside its endemic region and the fact that the <jats:styled-content style=\"fixed-case\">LAV<\/jats:styled-content> is not licensed for use elsewhere in the world, have spurred attempts to develop an alternative safer, yet cost\u2010effective recombinant vaccine. Here, we report the plant\u2010based production of a virus\u2010like particle (<jats:styled-content style=\"fixed-case\">VLP<\/jats:styled-content>) <jats:styled-content style=\"fixed-case\">AHSV<\/jats:styled-content> serotype five candidate vaccine by <jats:italic>Agrobacterium tumefaciens<\/jats:italic>\u2010mediated transient expression of all four capsid proteins in <jats:italic>Nicotiana benthamiana<\/jats:italic> using the cowpea mosaic virus\u2010based <jats:italic>HyperTrans<\/jats:italic> (<jats:styled-content style=\"fixed-case\">CPMV<\/jats:styled-content>\u2010<jats:italic><jats:styled-content style=\"fixed-case\">HT<\/jats:styled-content><\/jats:italic>) and associated <jats:styled-content style=\"fixed-case\">pEAQ<\/jats:styled-content> plant expression vector system. The production process is fast and simple, scalable, economically viable, and most importantly, guinea pig antiserum raised against the vaccine was shown to neutralize live virus in cell\u2010based assays. To our knowledge, this is the first report of <jats:styled-content style=\"fixed-case\">AHSV VLP<\/jats:styled-content>s produced in plants, which has important implications for the containment of, and fight against the spread of, this deadly disease.<\/jats:p>","DOI":"10.1111\/pbi.12783","type":"journal-article","created":{"date-parts":[[2017,6,26]],"date-time":"2017-06-26T10:24:40Z","timestamp":1498472680000},"page":"442-450","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Immunogenicity of plant\u2010produced African horse sickness virus\u2010like particles: implications for a novel vaccine"],"prefix":"10.1111","volume":"16","author":[{"given":"Susan J.","family":"Dennis","sequence":"first","affiliation":[{"name":"Biopharming Research Unit Department of Molecular and Cell Biology University of Cape Town Cape Town South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ann E.","family":"Meyers","sequence":"additional","affiliation":[{"name":"Biopharming Research Unit Department of Molecular and Cell Biology University of Cape Town Cape Town South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alan J.","family":"Guthrie","sequence":"additional","affiliation":[{"name":"Equine Research Centre University of Pretoria Onderstepoort South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Inga I.","family":"Hitzeroth","sequence":"additional","affiliation":[{"name":"Biopharming Research Unit Department of Molecular and Cell Biology University of Cape Town Cape Town South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edward P.","family":"Rybicki","sequence":"additional","affiliation":[{"name":"Biopharming Research Unit Department of Molecular and Cell Biology University of Cape Town Cape Town South Africa"},{"name":"Institute of Infectious Disease and Molecular Medicine Faculty of Health Sciences University of Cape Town Cape Town South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,8]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2014.04.036"},{"key":"e_1_2_6_3_1","first-page":"375","article-title":"The immunization of horses and mules against Horse Sickness by means of the neurotropic virus of mice and guinea\u2010pigs","volume":"2","author":"Alexander R.A.","year":"1934","journal-title":"Onderstepoort. 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