{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T21:35:50Z","timestamp":1791408950004,"version":"4.3.3"},"reference-count":21,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2017,11,24]],"date-time":"2017-11-24T00:00:00Z","timestamp":1511481600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003329","name":"Ministerio de Econom\u00eda y Competitividad","doi-asserted-by":"publisher","award":["AGL2013\u201048946\u2010C3\u20101\u2010R"],"award-info":[{"award-number":["AGL2013\u201048946\u2010C3\u20101\u2010R"]}],"id":[{"id":"10.13039\/501100003329","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003329","name":"Ministerio de Econom\u00eda y Competitividad","doi-asserted-by":"publisher","award":["AGL2016\u201080566\u2010P"],"award-info":[{"award-number":["AGL2016\u201080566\u2010P"]}],"id":[{"id":"10.13039\/501100003329","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008530","name":"European Regional Development Fund","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Plant Biotechnology Journal"],"published-print":{"date-parts":[[2018,4]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>Coeliac disease is an autoimmune disorder triggered in genetically predisposed individuals by the ingestion of gluten proteins from wheat, barley and rye. The \u03b1\u2010gliadin gene family of wheat contains four highly stimulatory peptides, of which the 33\u2010mer is the main immunodominant peptide in patients with coeliac. We designed two sgRNAs to target a conserved region adjacent to the coding sequence for the 33\u2010mer in the \u03b1\u2010gliadin genes. Twenty\u2010one mutant lines were generated, all showing strong reduction in \u03b1\u2010gliadins. Up to 35 different genes were mutated in one of the lines of the 45 different genes identified in the wild type, while immunoreactivity was reduced by 85%. Transgene\u2010free lines were identified, and no off\u2010target mutations have been detected in any of the potential targets. The low\u2010gluten, transgene\u2010free wheat lines described here could be used to produce low\u2010gluten foodstuff and serve as source material to introgress this trait into elite wheat varieties.<\/jats:p>","DOI":"10.1111\/pbi.12837","type":"journal-article","created":{"date-parts":[[2017,9,18]],"date-time":"2017-09-18T10:37:59Z","timestamp":1505731079000},"page":"902-910","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":557,"title":["Low\u2010gluten, nontransgenic wheat engineered with CRISPR\/Cas9"],"prefix":"10.1111","volume":"16","author":[{"given":"Susana","family":"S\u00e1nchez\u2010Le\u00f3n","sequence":"first","affiliation":[{"name":"Departamento de Mejora Gen\u00e9tica Vegetal Instituto de Agricultura Sostenible (IAS\u2010CSIC) C\u00f3rdoba Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Javier","family":"Gil\u2010Humanes","sequence":"additional","affiliation":[{"name":"Department of Genetics Cell Biology, and Development Center for Genome Engineering University of Minnesota Minneapolis MN USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carmen V.","family":"Ozuna","sequence":"additional","affiliation":[{"name":"Departamento de Mejora Gen\u00e9tica Vegetal Instituto de Agricultura Sostenible (IAS\u2010CSIC) C\u00f3rdoba Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda J.","family":"Gim\u00e9nez","sequence":"additional","affiliation":[{"name":"Departamento de Mejora Gen\u00e9tica Vegetal Instituto de Agricultura Sostenible (IAS\u2010CSIC) C\u00f3rdoba Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolina","family":"Sousa","sequence":"additional","affiliation":[{"name":"Departamento de Microbiolog\u00eda y Parasitolog\u00eda Facultad de Farmacia Universidad de Sevilla Sevilla Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel F.","family":"Voytas","sequence":"additional","affiliation":[{"name":"Department of Genetics Cell Biology, and Development Center for Genome Engineering University of Minnesota Minneapolis MN USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francisco","family":"Barro","sequence":"additional","affiliation":[{"name":"Departamento de Mejora Gen\u00e9tica Vegetal Instituto de Agricultura Sostenible (IAS\u2010CSIC) C\u00f3rdoba Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,11,24]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/pbi.12455"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btq461"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btv401"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcs.2008.03.005"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1007773107"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/err119"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcs.2012.02.009"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0090898"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0091931"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkf436"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1467-7652.2011.00658.x"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcn\/87.2.405"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.3109\/07853890.2010.505931"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/tpj.12851"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11032-009-9263-1"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0024754"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0009490"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1186\/1741-7015-9-23"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.3001012"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.meg.0000059119.41030.df"},{"key":"e_1_2_7_22_1","unstructured":"Williams P. Jaby el\u2010Haramein F. Nakkoul H.andRihawi S.(1986)Crop quality evaluation methods and guidelines. International Center for Agricultural Research in the Dry Areas"}],"container-title":["Plant Biotechnology Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fpbi.12837","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/pbi.12837","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,24]],"date-time":"2023-09-24T11:34:37Z","timestamp":1695555277000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/pbi.12837"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,11,24]]},"references-count":21,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2018,4]]}},"alternative-id":["10.1111\/pbi.12837"],"URL":"https:\/\/doi.org\/10.1111\/pbi.12837","archive":["Portico"],"relation":{},"ISSN":["1467-7644","1467-7652"],"issn-type":[{"value":"1467-7644","type":"print"},{"value":"1467-7652","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,11,24]]},"assertion":[{"value":"2017-05-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-08-26","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-11-24","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}