{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T11:54:04Z","timestamp":1790682844752,"version":"4.1.0"},"reference-count":66,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2017,11,9]],"date-time":"2017-11-09T00:00:00Z","timestamp":1510185600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31371658"],"award-info":[{"award-number":["31371658"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Plant Biotechnology Journal"],"published-print":{"date-parts":[[2018,4]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">WRKY<\/jats:styled-content> transcription factors are known to participate in the defence responses of higher plants. However, little is known about the roles of such proteins, especially regarding their functions in the resistance of oilseed rape (<jats:italic>Brassica napus<\/jats:italic>) to <jats:italic>Sclerotinia sclerotiorum<\/jats:italic>, a necrotrophic fungal pathogen that causes stem rot. In this study, we identified <jats:italic>Bn<jats:styled-content style=\"fixed-case\">WRKY<\/jats:styled-content>33<\/jats:italic> as a <jats:italic>S.\u00a0sclerotiorum<\/jats:italic>\u2010responsive gene that positively regulates resistance to this pathogen by enhancing the expression of genes involved in camalexin synthesis and genes regulated by salicylic acid (<jats:styled-content style=\"fixed-case\">SA<\/jats:styled-content>) and jasmonic acid (<jats:styled-content style=\"fixed-case\">JA<\/jats:styled-content>). We also identified a <jats:italic>S.\u00a0sclerotiorum<\/jats:italic>\u2010responsive region in the promoter of <jats:italic>Bn<jats:styled-content style=\"fixed-case\">WRKY<\/jats:styled-content>33<\/jats:italic>, which we revealed to be a relatively conserved W\u2010box region in the promoters of homologous genes in different species. Using this <jats:italic>S.\u00a0sclerotiorum<\/jats:italic>\u2010responsive region as bait in a yeast one\u2010hybrid assay, we identified another <jats:styled-content style=\"fixed-case\">WRKY<\/jats:styled-content> transcription factor, <jats:italic>Bn<jats:styled-content style=\"fixed-case\">WRKY<\/jats:styled-content>15<\/jats:italic>, and observed that both Bn<jats:styled-content style=\"fixed-case\">WRKY<\/jats:styled-content>15 and Bn<jats:styled-content style=\"fixed-case\">WRKY<\/jats:styled-content>33 could bind to this region. In addition, <jats:italic>Bn<jats:styled-content style=\"fixed-case\">WRKY<\/jats:styled-content>15<\/jats:italic> overexpression simultaneously increased the susceptibility of <jats:italic>B<\/jats:italic>.\u00a0<jats:italic>napus<\/jats:italic> to <jats:italic>S.\u00a0sclerotiorum<\/jats:italic> and down\u2010regulated <jats:italic>Bn<jats:styled-content style=\"fixed-case\">WRKY<\/jats:styled-content>33<\/jats:italic> after different durations of infection. Furthermore, Bn<jats:styled-content style=\"fixed-case\">WRKY<\/jats:styled-content>15, which contains a transcriptional repression domain, exhibited reduced transactivation ability and could reduce the transactivation ability of Bn<jats:styled-content style=\"fixed-case\">WRKY<\/jats:styled-content>33 in <jats:italic>Arabidopsis<\/jats:italic> protoplast assays. Therefore, we suggest that the increased susceptibility of <jats:italic>Bn<jats:styled-content style=\"fixed-case\">WRKY<\/jats:styled-content>15<\/jats:italic>\u2010overexpressing plants results from reduced <jats:italic>Bn<jats:styled-content style=\"fixed-case\">WRKY<\/jats:styled-content>33<\/jats:italic> expression, which is due to the inhibition of Bn<jats:styled-content style=\"fixed-case\">WRKY<\/jats:styled-content>33 transcriptional activation by Bn<jats:styled-content style=\"fixed-case\">WRKY<\/jats:styled-content>15.<\/jats:p>","DOI":"10.1111\/pbi.12838","type":"journal-article","created":{"date-parts":[[2017,9,20]],"date-time":"2017-09-20T07:23:02Z","timestamp":1505892182000},"page":"911-925","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":85,"title":["Interactions of <i><scp>WRKY<\/scp>15<\/i> and <i><scp>WRKY<\/scp>33<\/i> transcription factors and their roles in the resistance of oilseed rape to <i>Sclerotinia<\/i> infection"],"prefix":"10.1111","volume":"16","author":[{"given":"Fei","family":"Liu","sequence":"first","affiliation":[{"name":"National Key Lab of Crop Genetic Improvement, National Center of Rapeseed Improvement in Wuhan College of Plant Science and Technology Huazhong Agricultural University Wuhan Hubei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoxia","family":"Li","sequence":"additional","affiliation":[{"name":"National Key Lab of Crop Genetic Improvement, National Center of Rapeseed Improvement in Wuhan College of Plant Science and Technology Huazhong Agricultural University Wuhan Hubei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meirong","family":"Wang","sequence":"additional","affiliation":[{"name":"National Key Lab of Crop Genetic Improvement, National Center of Rapeseed Improvement in Wuhan College of Plant Science and Technology Huazhong Agricultural University Wuhan Hubei 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