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The recently licensed Sanofi Pasteur Dengvaxia vaccine does not protect children under the age of nine, and additional vaccine strategies are thus needed to halt this expanding global epidemic. Here, we employed a molecular engineering approach and plant expression to produce a humanized and highly immunogenic poly\u2010immunoglobulin G scaffold (<jats:styled-content style=\"fixed-case\">PIGS<\/jats:styled-content>) fused to the consensus dengue envelope protein III domain (c<jats:styled-content style=\"fixed-case\">EDIII<\/jats:styled-content>). The immunogenicity of this IgG Fc receptor\u2010targeted vaccine candidate was demonstrated in transgenic mice expressing human Fc\u03b3RI\/CD64, by induction of neutralizing antibodies and evidence of cell\u2010mediated immunity. Furthermore, these molecules were able to prime immune cells from human adenoid\/tonsillar tissue <jats:italic>ex\u00a0vivo<\/jats:italic> as evidenced by antigen\u2010specific CD4<jats:sup>+<\/jats:sup> and CD8<jats:sup>+<\/jats:sup> T\u2010cell proliferation, IFN\u2010\u03b3 and antibody production. The purified polymeric fraction of dengue PIGS (D\u2010<jats:styled-content style=\"fixed-case\">PIGS<\/jats:styled-content>) induced stronger immune activation than the monomeric form, suggesting a more efficient interaction with the low\u2010affinity Fc\u03b3 receptors on antigen\u2010presenting cells. These results show that the plant\u2010expressed D\u2010<jats:styled-content style=\"fixed-case\">PIGS<\/jats:styled-content> have the potential for translation towards a safe and easily scalable single antigen\u2010based tetravalent dengue vaccine.<\/jats:p>","DOI":"10.1111\/pbi.12869","type":"journal-article","created":{"date-parts":[[2017,12,10]],"date-time":"2017-12-10T00:42:40Z","timestamp":1512866560000},"page":"1283-1294","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["Plant\u2010expressed Fc\u2010fusion protein tetravalent dengue vaccine with inherent adjuvant properties"],"prefix":"10.1111","volume":"16","author":[{"given":"Mi Young","family":"Kim","sequence":"first","affiliation":[{"name":"Institute for Infection and Immunity St George's University of London London UK"},{"name":"Department of Molecular Biology and the Institute for Molecular Biology and Genetics Chonbuk National University Jeonju 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