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Outbreaks of <jats:styled-content style=\"fixed-case\">CSF<\/jats:styled-content> in Europe, Asia, Africa and South America had significant adverse impacts on animal health, food security and the pig industry. The disease is generally contained by prevention of exposure through import restrictions (<jats:italic>e.g<\/jats:italic>. banning import of live pigs and pork products), localized vaccination programmes and culling of infected or at\u2010risk animals, often at very high cost. Current <jats:styled-content style=\"fixed-case\">CSFV<\/jats:styled-content>\u2010modified live virus vaccines are protective, but do not allow differentiation of infected from vaccinated animals (<jats:styled-content style=\"fixed-case\">DIVA<\/jats:styled-content>), a critical aspect of disease surveillance programmes. Alternatively, first\u2010generation subunit vaccines using the viral protein E2 allow for use of <jats:styled-content style=\"fixed-case\">DIVA<\/jats:styled-content> diagnostic tests, but are slow to induce a protective response, provide limited prevention of vertical transmission and may fail to block viral shedding. <jats:styled-content style=\"fixed-case\">CSFV<\/jats:styled-content> E2 subunit vaccines from a baculovirus\/insect cell system have been developed for several vaccination campaigns in Europe and Asia. However, this expression system is considered expensive for a veterinary vaccine and is not ideal for wide\u2010spread deployment. To address the issues of scalability, cost of production and immunogenicity, we have employed an <jats:italic>Agrobacterium<\/jats:italic>\u2010mediated transient expression platform in <jats:italic>Nicotiana benthamiana<\/jats:italic> and formulated the purified antigen in novel oil\u2010in\u2010water emulsion adjuvants. We report the manufacturing of adjuvanted, plant\u2010made <jats:styled-content style=\"fixed-case\">CSFV<\/jats:styled-content> E2 subunit vaccine. The vaccine provided complete protection in challenged pigs, even after single\u2010dose vaccination, which was accompanied by strong virus neutralization antibody responses.<\/jats:p>","DOI":"10.1111\/pbi.12986","type":"journal-article","created":{"date-parts":[[2018,7,11]],"date-time":"2018-07-11T11:00:46Z","timestamp":1531306846000},"page":"410-420","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["Plant\u2010made E2 glycoprotein single\u2010dose vaccine protects pigs against classical swine fever"],"prefix":"10.1111","volume":"17","author":[{"given":"Richard C.","family":"Laughlin","sequence":"first","affiliation":[{"name":"Department of Biological and Health Sciences Texas A&amp;M University Kingsville Kingsville TX USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rachel","family":"Madera","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Physiology Kansas State University Manhattan KS USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yair","family":"Peres","sequence":"additional","affiliation":[{"name":"iBio CDMO Bryan TX USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brian R.","family":"Berquist","sequence":"additional","affiliation":[{"name":"iBio CDMO Bryan TX USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lihua","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Physiology Kansas State University Manhattan KS USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sterling","family":"Buist","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Physiology Kansas State University Manhattan KS USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yulia","family":"Burakova","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Physiology Kansas State University Manhattan KS USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sreenath","family":"Palle","sequence":"additional","affiliation":[{"name":"iBio CDMO Bryan TX USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chungwon J.","family":"Chung","sequence":"additional","affiliation":[{"name":"U.S. Department of Homeland Security Science and Technology Directorate Plum Island Animal Disease Center Greenport New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Max V.","family":"Rasmussen","sequence":"additional","affiliation":[{"name":"U.S. Department of Homeland Security Science and Technology Directorate Plum Island Animal Disease Center Greenport New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erica","family":"Martel","sequence":"additional","affiliation":[{"name":"Oak Ridge Institute for Science and Education Plum Island Animal Disease Center Research Participation Program Oak Ridge TN USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David A.","family":"Brake","sequence":"additional","affiliation":[{"name":"BioQuest Associates LLC Plum Island Animal Disease Center Greenport New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John G.","family":"Neilan","sequence":"additional","affiliation":[{"name":"U.S. Department of Homeland Security Science and Technology Directorate Plum Island Animal Disease Center Greenport New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara D.","family":"Lawhon","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pathobiology Texas A&amp;M University College Station TX USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L. 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