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There is a significant economic strain on the global swine industry due to <jats:styled-content style=\"fixed-case\">PCAD<\/jats:styled-content> and the production of commercial <jats:styled-content style=\"fixed-case\">PCV<\/jats:styled-content>\u20102 vaccines is expensive. Plant expression systems are increasingly regarded as a viable technology to produce recombinant proteins for use as pharmaceutical agents and vaccines. However, successful production and purification of <jats:styled-content style=\"fixed-case\">PCV<\/jats:styled-content>\u20102 capsid protein (<jats:styled-content style=\"fixed-case\">CP<\/jats:styled-content>) from plants is an essential first step towards the goal of a plant\u2010produced <jats:styled-content style=\"fixed-case\">PCV<\/jats:styled-content>\u20102 vaccine candidate. In this study, the <jats:styled-content style=\"fixed-case\">PCV<\/jats:styled-content>\u20102 <jats:styled-content style=\"fixed-case\">CP<\/jats:styled-content> was transiently expressed in <jats:italic>Nicotiana benthamiana<\/jats:italic> plants via agroinfiltration and <jats:styled-content style=\"fixed-case\">PCV<\/jats:styled-content>\u20102 <jats:styled-content style=\"fixed-case\">CP<\/jats:styled-content> was successfully purified using sucrose gradient ultracentrifugation. The <jats:styled-content style=\"fixed-case\">CP<\/jats:styled-content> self\u2010assembled into virus\u2010like particles (<jats:styled-content style=\"fixed-case\">VLP<\/jats:styled-content>s) resembling native virions and up to 6.5\u00a0mg of <jats:styled-content style=\"fixed-case\">VLP<\/jats:styled-content>s could be purified from 1\u00a0kg of leaf wet weight. Mice immunized with the plant\u2010produced <jats:styled-content style=\"fixed-case\">PCV<\/jats:styled-content>\u20102 <jats:styled-content style=\"fixed-case\">VLP<\/jats:styled-content>s elicited specific antibody responses to <jats:styled-content style=\"fixed-case\">PCV<\/jats:styled-content>\u20102 <jats:styled-content style=\"fixed-case\">CP<\/jats:styled-content>. This is the first report describing the expression of <jats:styled-content style=\"fixed-case\">PCV<\/jats:styled-content>\u20102 <jats:styled-content style=\"fixed-case\">CP<\/jats:styled-content> in plants, the confirmation of its assembly into <jats:styled-content style=\"fixed-case\">VLP<\/jats:styled-content>s and the demonstration of their use to elicit a strong immune response in a mammalian model.<\/jats:p>","DOI":"10.1111\/pbi.13097","type":"journal-article","created":{"date-parts":[[2019,2,21]],"date-time":"2019-02-21T19:02:50Z","timestamp":1550775770000},"page":"1751-1759","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Immunogenicity of plant\u2010produced porcine circovirus\u2010like particles in mice"],"prefix":"10.1111","volume":"17","author":[{"given":"Cornelius J.","family":"Gunter","sequence":"first","affiliation":[{"name":"Biopharming Research Unit Department of Molecular and Cell Biology University of Cape Town Cape Town South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guy L.","family":"Regnard","sequence":"additional","affiliation":[{"name":"Division of Medical Virology Department of Pathology and Institute of Infectious Diseases and Molecular Medicine Faculty of Health Sciences University of Cape Town Cape Town South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8024-9911","authenticated-orcid":false,"given":"Edward P.","family":"Rybicki","sequence":"additional","affiliation":[{"name":"Biopharming Research Unit Department of Molecular and Cell Biology University of Cape Town Cape Town South Africa"},{"name":"Institute of Infectious Disease and Molecular Medicine University of Cape Town Cape Town South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8515-9525","authenticated-orcid":false,"given":"Inga I.","family":"Hitzeroth","sequence":"additional","affiliation":[{"name":"Biopharming Research Unit Department of Molecular and Cell Biology University of Cape Town Cape Town South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,3,10]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2016.10.002"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1177\/104063870001200102"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2009.07.061"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2012.06.031"},{"key":"e_1_2_7_6_1","doi-asserted-by":"crossref","unstructured":"Chambers M.A. Graham S.P.andLa Ragione R.M.(2016)Challenges in Veterinary Vaccine Development and Immunization. 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