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Fe\u2010fortification of food is challenging because soluble Fe is unstable and insoluble Fe is nonbioavailable. Genetic engineering is an alternative approach for Fe\u2010biofortification, but so far strategies to increase Fe content have only encompassed a few genes with limited success. In this study, we demonstrate that the ethyl methanesulfonate (EMS) mutant, <jats:italic>iron deficiency tolerant1<\/jats:italic> (<jats:italic>idt1<\/jats:italic>), can accumulate 4\u20137 times higher amounts of Fe than the wild type in roots, shoots and seeds, and exhibits the metal tolerance and iron accumulation (Metina) phenotype in Arabidopsis. Fe\u2010regulated protein stability and nuclear localisation of the upstream transcriptional regulator bHLH34 were uncovered. The C to T transition mutation resulting in substitution of alanine to valine at amino acid position 320 of bHLH34 (designated as IDT1<jats:sub>A320V<\/jats:sub>) in a conserved motif among mono\u2010 and dicots was found to be responsible for a dominant phenotype that possesses constitutive activation of the Fe regulatory pathway. Overexpression of <jats:italic>IDT1<jats:sub>A320V<\/jats:sub><\/jats:italic> in Arabidopsis and tobacco led to the Metina phenotype; a phenotype that has escalated specificity towards optimising Fe homeostasis and may be useful in Fe\u2010biofortification. Knowledge of the high tolerance and accumulation of heavy metals of this mutant can aid the development of tools for phytoremediation of contaminants.<\/jats:p>","DOI":"10.1111\/pbi.13285","type":"journal-article","created":{"date-parts":[[2019,10,31]],"date-time":"2019-10-31T20:47:23Z","timestamp":1572554843000},"page":"1200-1210","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["The dual benefit of a dominant mutation in Arabidopsis <i>IRON DEFICIENCY TOLERANT1<\/i> for iron biofortification and heavy metal phytoremediation"],"prefix":"10.1111","volume":"18","author":[{"given":"Reena","family":"Sharma","sequence":"first","affiliation":[{"name":"Agricultural Biotechnology Research Center Academia Sinica Taipei Taiwan"},{"name":"Molecular and Biological Agricultural Sciences Program Taiwan International Graduate Program Academia Sinica and National Chung Hsing University Taipei Taiwan"},{"name":"Graduate Institute of Biotechnology National Chung Hsing University Taichung Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3791-3423","authenticated-orcid":false,"given":"Kuo\u2010Chen","family":"Yeh","sequence":"additional","affiliation":[{"name":"Agricultural Biotechnology Research Center Academia Sinica Taipei Taiwan"},{"name":"Molecular and Biological Agricultural Sciences Program Taiwan International Graduate Program Academia Sinica and National Chung Hsing University Taipei Taiwan"},{"name":"Biotechnology Center National Chung Hsing University Taichung Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,11,20]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12298-013-0211-5"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(07)61690-0"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.4161\/fly.19695"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-313x.1998.00343.x"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.104.024315"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.001263"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/aob\/mcn207"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btr330"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3040.2005.01273.x"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2018.02.009"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2019.00006"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnut.2018.00012"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M113.514828"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41477-018-0266-y"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/tpj.13850"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.109.136374"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2019.e01914"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/cpmb.27"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.16.01916"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp352"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.15.01827"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.112.096636"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.110.074096"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1186\/1939-8433-6-40"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1021\/cr900112r"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-11171-7"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-018-0002-1"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1007\/4735_100"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/17800"},{"key":"e_1_2_8_31_1","volume-title":"Arabidopsis Protocols. 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